A New Multifunctional Hemostatic Dressing

[0022] 1. Preparation of chitosan with different degrees of chitosan; after chitin is treated with concentrated lye, the acetamide group in the molecule will be partially hydrolyzed and removed, and when more than half of the acetyl group in the molecule is removed, it will become chitosan. Because the acetamide group replaces the hydroxyl group on the C2 position of the chitin monomer, it is insoluble in dilute acid, dilute alkali and organic solvent, and its properties are similar to cellulose. When more than half of the acetyl groups are removed, it can be dissolved in acid. When chitosan is dissolved in an acidic solution, the free amine group (-NH2) on the monomer molecule is protonated and positively charged (-NH3+) becomes a cationic polymer, which can adsorb some negatively charged substances. In this experiment, further research on the coagulation and bacteriostasis of chitosan with different degrees of deacetylation in chitosan with a larger molecular weight (9.3 × 105 ~ 9.8 × 105), in order to construct a complex of collagen, chitosan and chrysophanol Provide theoretical basis for the multifunctional hemostatic dressing

[0023] 2. Preparation of modified and reconstructed collagen: control the chemical modification degree, molecular integrity, molecular weight and concentration of collagen; collagen type and content: type I collagen, non-collagen impurity content <1%. Melting temperature Tp: 50℃-80℃. The total amount of heavy metals: <10mg/kg. Sugar content: <1.0g/kg. Fat content: <0.1%. Ash: <1.0%. pH value: 7.0±0.4. Pyrogen: It should meet the qualification requirements of the Pyrogen Inspection Method in the Second Appendix of the Pharmacopoeia of the People's Republic of China, 2000 Edition. Hemolysis: Hemolysis rate ≤ 5%. Cytotoxicity: Cytotoxicity grade I-O.

[0024] 3. Purification of chrysophanol; obtain the effective substances in traditional Chinese medicine rhubarb for hemostasis, and quantitatively study it; first hydrolyze the total anthraquinone glycosides in rhubarb into aglycones with dilute sulfuric acid solution, then extract the aglycones with hot chloroform, and lye the chloroform solution with lye The rhein, emodin, and aloe-emodin were removed, and chrysophanol and emodin were separated by column chromatography, and recrystallized by ethanol for many times to obtain high-purity chrysophanol; high-performance liquid chromatography was used to determine the content of chrysophanol. content. The purity of chrysophanol is over 98%.

[0025] 4. Organically compound collagen, chitosan and chrysophanol to ensure the flexibility of the freeze-dried gasket composite sponge, and the outer membrane has tightness, adhesion and integrity: study the ratio of different collagen and chitosan The effect on hemostatic effect, and the anti-inflammatory, analgesic, and healing-promoting effects of compound chrysophanol after sustained release were studied through animal experiments.

[0026] The following examples are used to illustrate, but the application of the present invention is not limited to this.

[0027] Example 1. Preparation of a novel multifunctional hemostatic dressing. Corresponding chitosan, collagen and chrysophanol were prepared according to the above-mentioned steps (1) (2) (3), and compounded in different proportions according to the step (4). .

[0028] The obtained collagen, chitosan, chrysophanol composite material was cross-linked and freeze-dried, and then covered on the part of the mice with limb injury and bleeding. The hemostatic effect was observed, and it was found that the bleeding stopped after 1 minute. While simply using collagen as a hemostatic material to cover the bleeding site of the same injured mice, the bleeding stopped after 5 minutes. Simply use chitosan as a hemostatic material to cover the bleeding site of the same injured mice, and the bleeding stopped after 4 minutes. The bleeding site of the same injured mice was covered with a composite material of collagen and chitosan, and the bleeding stopped after 2 minutes.

[0029] Example 2. Preparation of a novel multifunctional hemostatic dressing. Corresponding chitosan, collagen and chrysophanol were prepared according to the above-mentioned steps (1) (2) (3), and compounded in different proportions according to step (4). .

[0030] After the obtained collagen, chitosan and chrysophanol composite materials were cross-linked and freeze-dried, the same culture conditions were used to culture staphylococcus and anaerobic bacteria. It was observed that the growth of bacteria was significantly inhibited after the composite material of collagen, chitosan and chrysophanol was cultured overnight, while the growth of bacteria on the pure collagen material was vigorous. The number of bacteria grown on the pure chitosan material was lower than that of the collagen group but higher than that of the composite material group of collagen, chitosan and chrysophanol. The number of bacterial growth on the composite material using collagen and chitosan is basically the same as that on the pure chitosan material.