Synthesis and application method of antigens for various heavy metals

A synthesis method and heavy metal technology, applied in the field of environmental pollutant detection, can solve the problems of few reports on antigen synthesis and identification research, difficulty in finding synthesis and identification methods, and different technical details of the methods, so as to ensure food safety and people's safety. Good health, good application prospects and significant effects

Active Publication Date: 2011-11-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] After searching the prior art, it was found that in recent years, the heavy metal Cd 2+ , Hg 2+ , Pb 2+ , U 6+ 、Cu 2+ , Zn 2+ There are certain research reports on antibodies such as ect., and the bifunctional chelating agents they use to synthesize antigens include derivatives of EDTA, DTPA, etc., but the general focus is on the preparation of antibodies, and there are few reports on the synthesis and identification of antigens. The technical details of the methods used are also different, and it is difficult to find a general method for the synthesis and identification of various heavy metal antigens

Method used

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  • Synthesis and application method of antigens for various heavy metals
  • Synthesis and application method of antigens for various heavy metals
  • Synthesis and application method of antigens for various heavy metals

Examples

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Embodiment 1

[0021] Preparation of heavy metal cadmium complete antigen

[0022] Such as figure 1 As shown, it is the steps of this example, wherein: the amino group on the benzene ring of the bifunctional chelating agent p-NH2-Bn-DTPA and the amino group on the carrier protein are connected through glutaraldehyde to chelate heavy metals to synthesize artificial antigens. The details are as follows:

[0023] Prepare p-NH2-Bn-DTPA 10mg dissolved in 3mL HEPES buffer (10mM, pH 7.4), carrier protein BSA15mg or KLH 30mg dissolved in 6mL HEPES buffer, glutaraldehyde (GA) 50% aqueous solution with HEPES buffer 100 times Dilute, slowly add the p-NH2-Bn-DTPA solution to the carrier protein solution at room temperature, stir while dropping, then slowly add 1 mL of diluted GA into the reaction solution, stir while dropping, and stir overnight at room temperature . The next day, the reaction solution was colorless or light yellow and transparent. Use a 15mL centrifugal ultrafiltration tube (molecul...

Embodiment 2

[0026] Preparation of heavy metal lead complete antigen

[0027] After dissolving 10mg p-NH2-Bn-DTPA, add dropwise to carrier protein BSA 15mg or KLH 30mg, then add dropwise 100 times diluted 50% glutaraldehyde aqueous solution 1mL, and stir at room temperature overnight. Use a 15mL centrifugal ultrafiltration tube for ultrafiltration washing 3 times, after dilution, add 2mL of 1mg / mL heavy metal lead solution dropwise, and stir at room temperature for 5 hours. The reacted solution was ultrafiltered and washed three times with a 15 mL centrifugal ultrafiltration tube to obtain the heavy metal lead complete immunogen Pb-DTPA-KLH and the coated detection original Pb-DTPA-BSA.

Embodiment 3

[0029] Preparation of Complete Antigen of Heavy Metal Mercury

[0030] After dissolving 10mg p-NH2-Bn-DTPA, add dropwise to carrier protein BSA 15mg or OVA 10mg, then add dropwise 100 times diluted 50% glutaraldehyde aqueous solution 1mL, and stir at room temperature overnight. Use a 15mL centrifugal ultrafiltration tube for ultrafiltration washing 3 times, after dilution, add 2mL of 1mg / mL heavy metal mercury solution dropwise, and stir at room temperature for 5 hours. The reacted solution was ultrafiltered and washed three times with a 15 mL centrifugal ultrafiltration tube to obtain the heavy metal mercury complete immunogen Hg-DTPA-BSA and the coated detection original Hg-DTPA-OVA.

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Abstract

The invention provides a synthesis and application method of an antigen for multiple heavy metals in the technical field of environment pollution detection. The molecular structure of the antigen is as follows: according to the invention, complete antigens of multiple heavy metals including cadmium, lead, mercury, zinc and the like can be synthesized; and the complete antigens are purified and represented to identify key parameters of the structures and coupling ratio so that a simple, rapid and effective heavy metal antigen synthesis method is established.

Description

technical field [0001] The invention relates to a method in the technical field of environmental pollutant detection, in particular to a method for synthesizing and applying antigens of various heavy metals. Background technique [0002] Heavy metal pollution in the environment is a serious problem threatening human health. Various toxic and harmful heavy metals, including cadmium, lead, mercury, zinc, copper, etc., after polluting water bodies and agricultural products, accumulate in the human body at the top of the food chain through bioaccumulation and produce toxic effects. For example, heavy metal cadmium poisoning can cause "Itai-itai disease", lead poisoning damages nerves and organs, etc. To control the possible harm of heavy metal pollution to humans from the source, the most important thing is to effectively monitor and detect heavy metal contaminated samples (including soil, water and agricultural products, etc.). Traditional heavy metal detection methods such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K14/77C07K14/795C07K16/44G01N33/53G01N33/577
Inventor 周培奚涛邢海波支月娥詹学佳
Owner SHANGHAI JIAO TONG UNIV
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