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A kind of preparation method of universal donor liver

An all-purpose, hepatic technology, applied in the field of clinical medical research, to solve the rejection reaction and expand the source of donors

Inactive Publication Date: 2011-12-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the galactase in coffee beans is very little. The enzyme extracted from 50 pounds of coffee beans can only complete the transformation of 200 ml of blood type B into type O blood. 4,5

Method used

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  • A kind of preparation method of universal donor liver
  • A kind of preparation method of universal donor liver
  • A kind of preparation method of universal donor liver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Obtain cadaveric liver donors (hepatitis B positive or moderate-to-severe fatty liver are not clinically suitable for donor livers), and the blood group antigen type of the donor livers is detected as type A,

[0055] (2) Use 3000ml of kidney preservation solution at 15°C for washing and pretreatment, and then use preservation solution I for type A liver at 15°C to perfuse continuously for 5 hours through the hepatic artery, portal vein, and common bile duct, wherein: the preservation solution Ⅰ is made by adding 60mg α-N-acetylgalactosidase to 3000ml HTK solution,

[0056] (3) Liver tissue (100g) was taken to detect the changes of blood group antigen antibody in liver tissue, the general pathology under the microscope, and Annexin V flow cytometry to detect the apoptosis of liver cells. The test results are shown in Table 1 and figure 2 , Figure 4 shown.

Embodiment 2

[0062] (1) Obtain cadaveric liver donors (hepatitis B positive or moderate to severe fatty liver, etc. are not clinically suitable for donor livers), and the blood group antigen type of the donor livers is detected as type B,

[0063] (2) Use 3000ml of kidney preservation solution at 15°C for washing and pretreatment, and then use preservation solution II for type B liver at 15°C for 5 hours for continuous perfusion through the hepatic artery, portal vein, and common bile duct, wherein: the preservation solution II is made by adding 5mg α-galactosidase to 3000ml HTK solution,

[0064] (3) Liver tissue (100g) was taken to detect the changes of blood group antigen antibody in liver tissue, the general pathology under the microscope, and Annexin V flow cytometry to detect the apoptosis of liver cells. The test results are shown in Table 1 and figure 2 , Figure 4 shown.

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Abstract

The invention belongs to the field of clinical medicine researches, and particularly relates to a preparation method of a donor liver. The preparation method comprises the following steps: continuously perfusing type-A liver with a preservative fluid I at 15 DEG C for several hours through hepatic artery, portal vein and common bile duct; continuously perfusing type-B liver with a preservative fluid II at 15 DEG C for several hours through hepatic artery, portal vein and common bile duct; and continuously perfusing type-AB liver with the preservative fluid I and the preservative fluid II at 15 DEG C for several hours through hepatic artery, portal vein and common bile duct to obtain the liver with type-O blood group antigen, wherein the preservative fluid I is prepared by adding 60 mg alpha-N-acetyl galactosidase into 3,000 mL HTK (histidine-tryptophan-ketoglutarate) solution, and the preservative fluid II is prepared by adding 5 mg alpha-galactosidase into 3,000 mL HTK solution. The preparation method provided by the invention effectively solves problems such as transplantation of liver with incompatible ABO blood group.

Description

technical field [0001] The invention belongs to the field of clinical medical research, and in particular relates to a preparation method for converting a blood type antigen from a liver of type A, type B, or type AB into a type O universal donor liver. Background technique [0002] According to the report of the World Health Organization, there are currently about 350 million people in the world who are carriers of hepatitis B virus, and about 45 million people die of liver cirrhosis and 60 million people die of primary liver cancer every year. Among them, 30 million are chronic hepatitis, liver cirrhosis, and primary liver cancer, and as many as 300,000 people die of chronic liver disease every year. The prevailing wine culture and overloaded work, like a catalyst, promote the rapid progression of hepatitis to fulminant liver failure or liver cirrhosis, liver cancer. [0003] Liver transplantation has become recognized as the most effective treatment for end-stage liver d...

Claims

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Application Information

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IPC IPC(8): A61F2/02A61L27/36A01N1/02
Inventor 杨富春郑树森
Owner ZHEJIANG UNIV
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