Preparation method and application of silk fibroin compound hydroxyapatite material

A technology of compounding hydroxyapatite and hydroxyapatite, which is applied in the fields of medical science, prosthesis, tissue regeneration, etc., to achieve the effect of accelerating repair, treating continuous bone defects, and avoiding rejection

Inactive Publication Date: 2019-09-13
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the prior art, because the silk fibroin macromolecular chain is an amphipathic molecule, its solution has strong environmental sensitivity, temperature, pH, ultrasound, organic solvent and its own high concentration will cause the gelation of the silk fibroin aqueous solution. The greater the molecular weight of silk fibroin or the higher its concentration, the more likely it will cause gelation, and the concentration of the solution itself is one of the important factors affecting the mechanical properties of the material.

Method used

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  • Preparation method and application of silk fibroin compound hydroxyapatite material
  • Preparation method and application of silk fibroin compound hydroxyapatite material
  • Preparation method and application of silk fibroin compound hydroxyapatite material

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Embodiment 1

[0037] Preparation of silk fibroin aqueous solution (SF): silkworm cocoons were steamed with 0.005wt% sodium carbonate aqueous solution for 0.5h, and then degummed. The degummed silk was dissolved in 9.3mol / L lithium bromide solution, and the silk fibroin solution dissolved in lithium bromide was dialyzed with distilled water After 3 days, concentrate and process to obtain silk fibroin aqueous solutions with different concentrations, the concentrations of which are 6wt%, 9wt% and 12wt% respectively.

[0038] The specific concentration steps are as follows: put the purified low-concentration silk fibroin solution into a dialysis bag with a molecular weight cut-off of 8,000-14,000; hang the dialysis bag with the silk fibroin solution in an environment of 1-5°C, and use a fan to carry out the process. Purging accelerates the loss of water in the solution; take out the silk fibroin solution after fan blowing for 36 hours, and use the drying method to determine the concentration of ...

Embodiment 2

[0043] Scanning electron microscopy (SEM) was used to characterize the cross-sectional morphology characteristics of the three groups of silk fibroin composite hydroxyapatite materials synthesized in situ in Example 1. The sample preparation method is as follows: take unmineralized and mineralized silk fibroin film samples, brittle in liquid nitrogen, and then dry them under vacuum at -40°C, take appropriate size sample fragments, spray gold, and pass through the field. The hydrogel morphology was observed and photographed with an emission scanning electron microscope (FESEM, Zeiss, Germany), and the accelerating voltage of the scanning electron microscope was 7 kV. figure 2 The a-c shown are low-magnification (100×) cross-sectional scanning electron micrographs of enzyme-crosslinked silk fibroin materials prepared from raw material solutions with three concentrations of 6wt%, 9wt%, and 12wt%, and d-f are corresponding high-magnification (500×) The scanning electron micrograp...

Embodiment 3

[0045] The three groups of silk fibroin-composite hydroxyapatite materials prepared in Example 1 were respectively cut into powders, and were subjected to X-ray diffraction detection respectively to study the change of composite hydroxyapatite in the composite materials to the microscopic crystal morphology of the materials. image 3 As shown, the results show that the raw material of silk fibroin is an amorphous structure, and a certain crystallization (β-fold) appears after methanol regeneration. However, after the in situ mineralization of hydroxyapatite on the silk fibroin raw material, the characteristic peak of hydroxyapatite appeared, indicating the existence of hydroxyapatite.

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Abstract

The invention relates to a preparation method and application of a silk fibroin compound hydroxyapatite material. The preparation method comprises the following steps: 1, preparing a silk fibroin aqueous solution, and performing concentration; 2, preparing a mixed solution from the silk fibroin aqueous solution with a horseradish peroxidase solution and hydrogen peroxide; 3, molding the mixed solution, performing crosslinking in an environment at the temperature of 28-45 DEG C, and performing freezing treatment to obtain a silk fibroin primary molding product; 4, soaking the silk fibroin primary molding product in a methanol solution, performing rinsing to obtain silk fibroin molding product; and 5, synthesizing nano-hydroxyapatite particles on the silk fibroin molding product by using anin-situ mineralization method to obtain the silk fibroin compound hydroxyapatite material. The preparation method can prepare silk fibroin compound hydroxyapatite bone duct by adopting a method with simple operation, high yield and no toxicity, and the silk fibroin compound hydroxyapatite bone duct can be used for effectively treating continuous bone defects and can avoid risks in autogenous boneextraction and rejection reaction of allogeneic bones.

Description

technical field [0001] The invention relates to the technical fields of material chemistry and medical polymer functional materials, in particular to a preparation method and application of a silk fibroin composite hydroxyapatite material. Background technique [0002] In modern research on bone injury repair, how to rebuild bone and promote bone healing must be considered from four basic elements: ①Osteoconductive matrix; ②Osteoinductive factors; Can directly form new bone; ④ stable biomechanical environment. At present, in clinical practice, autologous bone or allogeneic bone is often used as the main treatment. Autologous bone therapy has always been considered the best choice for bone grafting, but autologous bone materials are limited, blood loss will inevitably increase when cutting the bone for transplantation, the operation time will increase and the patient will suffer, and the donor site will also cause some discomfort. There may even be complications. However, ...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/12A61L27/50
CPCA61L27/12A61L27/227A61L27/50A61L2430/02C08L89/00
Inventor 常春雨徐瑞郑博文肖凌飞蔡林张俐娜
Owner WUHAN UNIV
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