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Method for extracting and purifying sarmentosin in Sedum sarmentosum Bunge

A technology of glucoside and purification method, which is applied in the field of medicine, can solve problems such as inability to industrialize, low yield, and complexity, and achieve the effects of reducing energy consumption and improving product purity

Inactive Publication Date: 2012-01-25
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are relatively complicated, and the utilization rate of medicinal active ingredients is low, so they cannot be industrialized
Chinese Patent Application No. 201010511372.2 discloses a preparation method of glucoside. The method adopts fresh crocodile, soaks in water, squeezes and filters, adds activated carbon to the filtrate for decolorization, filters, takes the filtrate, and passes it through a macroporous adsorption resin column. Adsorption, eluting with 10-30% ethanol, collecting the eluate, recovering ethanol under reduced pressure, concentrating and drying, adding methanol to dissolve, filtering, taking the filtrate, adding it to a silica gel column chromatography, and using methanol at a ratio of 1:1 - Elution with ethyl acetate mixed solvent, the eluate is collected in fractions by column volume, the eluate is combined, concentrated under reduced pressure and dried at low temperature to obtain saponin, the yield of this method is low, only 0.05%-0.076%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Grind the herbaceous herb into coarse powder, pass through a 40-mesh sieve, weigh 5kg and put it into the percolation bucket, then add 5L of 20% ethanol aqueous solution to the percolation column, soak for 30min at 50°C, and keep it warm Open the outlet valve of the percolation column, and continuously add 20% ethanol aqueous solution from the top of the percolation column at the same time, control the flow rate to 10ml / min, until 30L percolation extract is collected, concentrate under reduced pressure until there is no alcohol smell, and then add water to 3L, stir evenly, add 45ml ZTC-Ⅱ traditional Chinese medicine clarifying agent under stirring, let it stand still, put the centrifuged filtrate on the polyamide column, take 2BV10% acetone to elute the impurities, and then use 50% acetone to elute the active ingredient, the elution flow rate For 4BV / h, collect the fractions of the glucoside, pass through the alumina column, collect the column liquid, concentrate under r...

Embodiment 2

[0021] Grind the herbaceous herb into coarse powder, pass through a 40-mesh sieve, weigh 5kg and put it into the percolation barrel, then add 3L of 40% acetone aqueous solution to the percolation column, soak for 40min at 55°C, and keep it warm Open the outlet valve of the percolation column, and continuously add 40% acetone aqueous solution from the top of the percolation column at the same time, control the flow rate to 15ml / min, until 20L percolation extract is collected, concentrate under reduced pressure until there is no acetone, and then add water to 5L, stir evenly, add 20ml ZTC-Ⅱ traditional Chinese medicine clarifier under stirring, let it stand still, put the centrifuged filtrate on the polyamide column, take 2BV15% acetone to elute the impurities, and then use 35% acetone to elute the active ingredients, the elution flow rate is 2BV / h, collect the glucoside fraction, pass through the alumina column, collect the column liquid, concentrate under reduced pressure to ob...

Embodiment 3

[0024] Grind the herbaceous weeds into coarse powder, pass through a 20-mesh sieve, weigh 5kg and put it into the percolation bucket, then add 4L of 30% ethanol aqueous solution to the percolation column, soak at 65°C for 45min, and keep it warm Open the outlet valve of the percolation column, and continuously add 30% ethanol aqueous solution from the top of the percolation column at the same time, control the flow rate to 10ml / min, until 25L percolation extract is collected, concentrate under reduced pressure until there is no alcohol smell, and then add water To 4L, stir evenly, add 55ml CTS traditional Chinese medicine clarifying agent under stirring, let it stand still, put the centrifuged filtrate on the polyamide column, take 1.0BV10% ethanol to elute the impurities, and then use 50% ethanol to elute the active ingredient, the elution flow rate is 6BV / h, collect the glucoside fraction, pass through the alumina column, collect the column liquid, concentrate under reduced ...

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Abstract

The invention belongs to the technical field of medicines and relates to a method for extracting and purifying sarmentosin in Sedum sarmentosum Bunge. The sarmentosin is obtained by the following steps of: extracting by using a water-soluble solvent, sedimenting by using a sedimentation agent, purifying by using a polyamide column, removing impurities by using an alumina column, and crystallizing. The content of the sarmentosin is over 90 percent; and compared with the conventional method, the invention has the advantages that: the method is simple and convenient, the using amount of an organic solvent is small, and the purity of products is high. The method is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a method for extracting and purifying glucoside from chinensis. Background technique [0002] Sedum sedum is a sedum sedum (Sedum sedum) Sedum sarmentosum Bunge), also known as Shijiao "Sichuan Traditional Chinese Medicine", Banzhilian "Medicine Mirror", Mouseya Banzhi "Baicao Mirror" and so on. Recorded in the "Chinese Pharmacopoeia" 2010 edition, it has the effect of clearing damp heat and detoxifying. For damp-heat jaundice, dysuria, carbuncle sores. "Compendium of Materia Medica Supplements" said that it "eliminates carbuncle and furuncle, diarrhea, jaundice, and ringworm of the throat". Chemical studies have shown that weeding grass mainly contains proteins, amino acids, sugars, flavonoids, triterpenoids and weeding glycosides. According to relevant literature reports, weeping grass can inhibit inflammatory exudation, reduce liver cell damage, and reduce s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/10C07H1/08
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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