Preparation method of hypotensive peptide of lactalbumin

Abstract

The invention relates to a preparation method of a hypotensive peptide of lactalbumin, belonging to the technical field of biology. The preparation method comprises the following steps of: maintaining 10min denaturation for lactalbumin suspension under the temperature of 85-90 DEG C, cooling to room temperature, adjusting the pH, then adding pepsin, reacting for 1-3 hours, deactivating enzyme, cooling, and centrifugating to take supernatant; adjusting the pH, adding trypsin and chymotrypsin, reacting for 4-6 hours, deactivating enzyme, cooling, and centrifugating to take supernatant; after vacuum concentration, freezing and drying to obtain a white or light-yellow crude product of the hypotensive peptide of the lactalbumin; and using DA201-C macroporous adsorption resin for adsorption, then carrying out gradient elution by alcohol, collecting and concentrating 75% of alcohol elution component, and utilizing glucan SephadexG-10 gel for chromatography separation to obtain the hypotensive peptide of the lactalbumin. The invention has the advantages that the preparation method is safe, has no toxic and side effects, has a simple process, and the industrial production is convenient; and the hypotensive peptide prepared by the invention can be easily absorbed by the human body and has high ACE (Angiotensin-converting Enzyme) inhibitory activity and has the characteristics of safety, no toxic and side effects, no action on normal blood pressure and the like, and can be used for preparing medicine and food for reducing blood pressure.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the preparation and primary separation technology of polypeptides. Background technique [0002] Antihypertensive peptides, also known as ACE inhibitory peptides (Angiotensin-converting Enzyme Inhibitory Peptides, ACEIP) is a class of small molecule peptides that can lower blood pressure in humans, it is an inhibitor of angiotensin-converting enzyme (Angiotensin-converting Enzyme, ACE) , can reduce blood pressure by inhibiting the conversion of angiotensin Ⅰ to angiotensin Ⅱ and preventing ACE from degrading bradykinin. Although a variety of chemically synthesized peptides can inhibit ACE activity, they all have certain side effects, and because their action time is not long, blood pressure will rebound after drug withdrawal, and a large number of experiments have proved that ACE inhibitory peptides derived from milk protein It has a high antihypertensive effect in vivo, mainly conc...

AI Technical Summary

Problems solved by technology

Although a variety of chemically synthesized peptides can inhibit ACE activity, they all have certain side effects, and because their action time is not long, blood pressure will rebound after drug withdrawal, and a large number of experiments have proved that ACE inhibitory peptides derived from milk protein Has a high antihypertensive effect in vivo, mainly concentrated in small molecule peptides