Batch preparation method for pattern coded microcarrier
A pattern coding and microcarrier technology, which can be applied to medical preparations containing active ingredients, pharmaceutical formulations, and antibody medical components, etc., can solve the problems of restricting the popularization and application of suspension array chips, and achieve rich pattern coding, low cost, and low cost. high yield effect
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Embodiment 1
[0034] First, a triangular micropit array with a pitch of 2000 microns, a depth of 50 microns, and a side length of 1000 microns is prepared on a square stainless steel plate with a side length of 10 cm by a laser. Then the needle of the contact spotting instrument connected with 20% (W / V) polystyrene toluene solution was sequentially spotted on the triangular micro-pit area on the stainless steel plate by contact method. After the toluene volatilizes and solidifies, polystyrene triangular flake microparticles are obtained. The triangular flaky micro-particles can be separated from the stainless steel plate by ultrasonically ultrasonicating the stainless steel plate in water, and the polystyrene triangular flaky micro-particles are dried for later use after cleaning. Square microparticles with a side length of 1000 microns and circular flake microparticles with a diameter of 1000 microns were prepared by similar methods. The polystyrene triangular sheet-shaped microparticles ...
Embodiment 2
[0036] Firstly, through a mask plate combined with photolithography technology, etch on the silicon wafer an array template of positive writing convex characters with a center interval of about 150 microns, a size of about 50 microns and a depth of about 10 microns. Then mix the basic components of Dow Corning SYLGARD 184 silicone rubber with the curing agent at a weight ratio of 10:1, pour it into a container containing a silicon wafer template with convex characters, evacuate until there are no bubbles, and store it at 100°C After curing for 1.5 hours, cooling and demoulding, a PDMS template containing an array of concave reverse characters A is obtained. The PDMS template is treated with methyltriethoxysilane vapor after being treated with oxygen plasma for 30 seconds, washed with ethanol, acetone, water and ethanol in sequence, and then dried for use. Then on the PDMS template that the methyl silane coupling agent has been processed, coat the positive photoresist and the p...
Embodiment 3
[0038]First, through a mask plate combined with photolithography technology, etch a square silicon wafer with a side length of 1 cm to etch a positive writing convex character A array template with a center interval of 150 microns, a size of 50 microns, and a depth of 10 microns. A cross alignment mark is reserved here. Mix the PDMS precursor and curing agent in a mass ratio of 10:1, remove the air bubbles, and cast it on a silicon wafer mold with an array of positive characters A, and place it in an oven for heating and curing. After demoulding, a PDMS template for reverse writing the concave character A array is obtained.
[0039] Then, a circular micropit array template with a center interval of 150 microns, a diameter of 50 microns and a depth of 100 microns is etched on another square silicon wafer with a side length of 1 cm through a mask plate combined with photolithography technology, and the template corresponds to the upper template. Cross alignment marks are also r...
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