Inkjet printing preparation method of pattern code microcarrier
A technology of pattern coding and microcarriers, applied in the direction of material inspection products, measuring devices, instruments, etc., can solve the problems of limitations and restrictions on the popularization and application of suspension array chips, and achieve the effects of low cost, large output, and rich coding
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Embodiment 1
[0027] First, the aqueous solution containing 40% ethylene glycol containing 20wt% cross-linked polymethyl methacrylate microspheres with a size ranging from 100 nm to 250 nm was placed on the transparencies (contact of the solution) by an Epson R230 inkjet printer. An array of characters A with a pitch of 500 microns and a size of 350 microns is printed on an angle of 80 degrees). After drying at room temperature, it was treated at 150 degrees Celsius for 1 hour, and then the A-shaped microparticles constructed of cross-linked polymethyl methacrylate were rinsed from the transparencies by deionized water and washed and dried. The obtained A-shaped cross-linked polymethyl methacrylate microparticles were first treated with ammonia plasma for 1 minute, then placed in 1% glutaraldehyde PB buffer at room temperature for 4 hours, and then washed with PBS buffer for 10 minutes. Wash three times in minutes. Then react with 0.2mg / ml hepatitis A antibody in PBS buffer solution at 4°C...
Embodiment 2
[0029] Prepare 15wt% polystyrene (Mn=100000) in toluene solution containing 10% 2-methylpyrrolidone as solution A. Then prepare an aqueous solution of 20wt% polyethylene glycol (Mn=100000) as solution B. A thin film of polystyrene forming the character X and polyethylene glycol forming the surrounding portion of the character was then printed on the polypropylene film by a Canon iP4760 inkjet printer. The character size is about 350 microns and the spacing is about 500 microns. After the film is dry, immerse the film in deionized water and slightly shake to dissolve the polyethylene glycol and separate the polystyrene microparticles from the polypropylene film, then the character X composed of polystyrene is dispersed in the water. Wash thoroughly with deionized water and dry to obtain X-character pattern coded microparticles made of polystyrene. The obtained X-shaped polystyrene microparticles were first treated with ammonia plasma for 1 minute, then placed in 1% glutaralde...
Embodiment 3
[0031]Add 500 ml of 5% aminopropyltriethoxysilane ethanol solution to 500 ml of ethanol colloid solution with a concentration of 10 wt % of silicon dioxide with a size between 90 nm and 110 nm, react at room temperature for 8 hours, centrifuge and wash with ethanol and disperse in A colloidal silica solution with a concentration of 1 wt% was formed in the PB buffer. Glutaraldehyde was added to the above-mentioned silica colloidal aqueous solution so that its concentration was 1% v / v, and then reacted at room temperature for 4 hours, centrifuged and washed three times with PBS buffer. Subsequently, the silica colloids linked with glutaraldehyde were dispersed in a PBS buffer solution containing 0.2 mg / ml hepatitis A virus at a final concentration of 1 wt%, reacted at 4 degrees Celsius for 12 hours, and centrifuged with 1% bovine serum albumin BSA was reacted at room temperature for 2 hours to block unreacted glutaraldehyde. Then, the silica colloid linked with hepatitis A anti...
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