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TRAP-SCAR585 label for identifying aegilops gametocidal chromosome 2C and application of same

A technology of Goatwort and chromosome, which is applied in the field of gametocidal chromosome identification, can solve the problems of inability to pass, cumbersome methods, semi-lethal, etc., and achieve the effect of improving the correct rate and improving accuracy

Inactive Publication Date: 2013-04-24
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromosome 2S lo , 2S sh , T2B-2S sp.au , 4S lo , 4S sh , 4S sh #2, causing complete sterility in "Chinese Spring" wheat in gametes without these chromosomes and therefore unable to be passed on to the next generation
Chromosome 3C exerts a strong gametocidal effect in 'Chinese Spring' but a mild, semi-lethal effect in other cultivars, suggesting the presence of repressor genes in these cultivars
Many scholars use the banding method to identify offspring, but this method is very cumbersome and requires the combination of related cytological techniques

Method used

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  • TRAP-SCAR585 label for identifying aegilops gametocidal chromosome 2C and application of same
  • TRAP-SCAR585 label for identifying aegilops gametocidal chromosome 2C and application of same
  • TRAP-SCAR585 label for identifying aegilops gametocidal chromosome 2C and application of same

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Embodiment Construction

[0031] The present invention provides a TRAP-SCAR for identifying gameticidal chromosome 2C of Capt. 585 The markers and their applications were screened by TRAP-SCAR method to obtain specific TRAP fragments, which were converted into stable SCAR markers, and their amplification was detected. Since the TRAP-SCAR method is used for screening, this marker can also be called TRAP-SCAR 585 mark. The preparation, amplification and identification of markers will be further described in detail below with reference to specific examples, which are to explain rather than limit the present invention.

[0032] Sources of material involved:

[0033] "Chinese Spring" wheat: It comes from the Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Harbin Normal University. In April 2010, the material was planted in the Key Experimental Nursery Base of Molecular Cytogenetics and Genetic Breeding of Harbin Normal University. Two months later, DNA was extracted for Molecular marker ...

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Abstract

The invention discloses a TRAP-SCAR585 label for identifying aegilops gametocidal chromosome 2C and application of the same. A nucleotide sequence of the aegilops gametocidal chromosome 2C is as shown in SEQ. ID. NO. 3 or SEQ. ID. NO. 6. In the invention, specific TRAP segments are screened to obtain through a TRAP-SCAR method, and are converted into stable SCAR labels; and amplification of the SCAR labels is detected. When the label of the aegilops gametocidal chromosome 2C provided by the invention is adopted, a solid foundation is laid for quickly identifying an exogenous chromatin or chromosome of the gametocidal chromosome 2C under the genetical background of wheat through the detection of the SCAR labels, and a very convenient and quick means is also provided for molecular marker-assisted breeding.

Description

technical field [0001] The present invention is a technical field of killing sub -chromosomal identification, involving a tag and application of identifying the crickets of the crict chromosome 2C. Background technique [0002] Wheat is one of the important food crops in the world. In China, it is the second largest crop that is second only to rice. Wheat production is of great strategic significance for the development of the national economy. Due to the single type of wheat cultivation, its genetic foundation has become increasingly narrow and scarce, which has greatly limited its further improvement of its output and quality. There are a large number of genetic variations in the close species of wheat. It is a beneficial genetic resource that can be used by wheat improvement. The introduction of these beneficial genes into wheat has always been a long -lasting research topic for geneticists and breeding scholars. Creating the void system is an effective way to import th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 郭长虹徐国辉束永俊苏文悦姜格格刘贺亮
Owner HARBIN NORMAL UNIVERSITY