Method for extracting favelanone

A technology of stingerone and nettle, which is applied in the field of extraction and preparation of active ingredients of traditional Chinese medicine, can solve the problems of no stingerone and other problems, and achieve the effects of avoiding troublesome operation, short production cycle, and facilitating large-scale production operations

Inactive Publication Date: 2012-03-28
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the method of preparing urticarone in large quantities in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Take 1 kg of nettle thorn bark and crush it into a coarse powder, put it into an extraction tank, add an appropriate amount of 80% ethanol, soak in a closed constant temperature below 60 °C for 2 hours, add solvent to 8 times the amount of the medicinal material, and perform ultrasonic extraction twice, each time Extracted for 60min, the extracts were combined and concentrated into an extract, and the extract was added from the top of the extraction tower, and the mixed solvent of cyclohexane-ethyl acetate with a volume ratio of 2:1 was selected as the extractant, and the ratio of the flow rate of the liquid to the material was 2 :1 is added from the bottom of the extraction tower, the two phases are mixed to complete the extraction, the extractant with urticarone is collected on the top, and the extractant is recovered by distillation under reduced pressure, and the Sephadex LH-20 column is used in a volume ratio of 10:1, 5: 1. 2:1 gradient elution, collect nettle keton...

Embodiment 2

[0014] Take 1 kg of nettle thorn bark and crush it into a coarse powder, put it into an extraction tank, add an appropriate amount of 85% ethanol, soak it in a closed constant temperature below 60°C for 3 hours, add solvent to 10 times the amount of the medicinal material, and perform ultrasonic extraction twice, each time Extracted for 150min, the extracts were combined and concentrated into an extract, and the extract was added from the top of the extraction tower, and the mixed solvent of cyclohexane-ethyl acetate with a volume ratio of 2:1 was selected as the extractant, and the ratio of the flow rate of the liquid to the material was 2 :1 is added from the bottom of the extraction tower, the two phases are mixed to complete the extraction, the extractant with urticarone is collected on the top, and the extractant is recovered by distillation under reduced pressure, and the Sephadex LH-20 column is used in a volume ratio of 10:1, 5: 1. 2:1 gradient elution, collect nettle k...

Embodiment 3

[0016] Take 1 kg of nettle thorn bark and crush it into a coarse powder and put it into an extraction tank, add an appropriate amount of 95% ethanol, soak it in a closed constant temperature below 60 °C for 1 hour, add solvent to 6 times the amount of the medicinal material, and perform ultrasonic extraction twice, each time Extracted for 30min, the extracts were combined and concentrated into an extract, and the extract was added from the top of the extraction tower, and the mixed solvent of cyclohexane-ethyl acetate with a volume ratio of 2:1 was selected as the extractant, and the ratio of the flow rate of the liquid to the material was 2 :1 is added from the bottom of the extraction tower, the two phases are mixed to complete the extraction, the extractant with urticarone is collected on the top, and the extractant is recovered by distillation under reduced pressure, and the Sephadex LH-20 column is used in a volume ratio of 10:1, 5: 1. 2:1 gradient elution, collect nettle ...

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PUM

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Abstract

The invention belongs to the field of the extraction and preparation of Chinese medicinal active ingredients, and relates to a method for extracting favelanone. The method comprises the following steps of: performing the ultrasound extraction of cnidoscolus phyllacanthus barks serving as raw materials by using ethanol aqueous solution, and performing countercurrent extraction, Sephadex LH-20 column chromatography, medium-pressure silica gel column chromatography and the like on concentrated extractum to obtain the favelanone. The method has a short production period, the obtained product has high purity, and the pharmacological clinical study of the favelanone is facilitated.

Description

technical field [0001] The invention belongs to the field of extraction and preparation of active ingredients of traditional Chinese medicines, and relates to a method for extracting urticarone. Background technique [0002] Nettle stingerone (Favelanone), the molecular formula is C 19 h 22 o 3 , with a molecular weight of 298.38, is a naphthoquinone compound, which is a colorless needle crystal (hexane), mp.152-153°C, and is mainly distributed in Euphorbiaceae (Euphorbiaceae) plants with stinging nettle stings Cnidoscolus phyllacanthus (Mart.) Pax et K. Hoffm. In bark. Pharmacological studies have shown that nettle ketone has cytotoxic activity, and its IC on P388 murine leukemia cells in vitro 50 It is 24.9μg / mL, and can be developed for anti-tumor drugs or health care products. At present, there is no report on the method of preparing urticarone in large quantities in China. Contents of the invention [0003] The object of the present invention is to provide a met...

Claims

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Application Information

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IPC IPC(8): C07C50/36C07C46/10
Inventor 刘东锋郭琴
Owner NANJING ZELANG MEDICAL TECH
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