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Bacillus subtilis, microorganism strain agent and microorganism fertilizer prepared by using bacillus subtilis as well as preparation methods thereof

A technology of Bacillus subtilis and microbial strains, applied in the field of microbial seed agents, can solve the problems of short shelf life, poor product quality, low survival ability of bacterial strains, etc., achieve long shelf life, high effective bacterial count, and solve problems with short shelf life. Effect

Inactive Publication Date: 2013-08-14
内蒙古鑫鑫盛达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems in the prior art such as low strain viability, substandard number of effective viable bacteria, short shelf life, poor product quality, unstable application effect, etc., one of the purposes of the present invention is to provide a new Bacillus subtilis bacillus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Using traditional methods to ferment Dr. Ma Xinku No. 1 to prepare microbial strains

[0050] Medium: 3% starch, 1% bean cake powder, 0.1% peptone, 0.2% ammonium sulfate, 0.2% dipotassium hydrogen phosphate, 0.1% yeast powder, 0.01% calcium carbonate, pH7.2-7.4.

[0051] After activating the strain, put it into 1000ml of the above-mentioned medium, and culture it in the Erlenmeyer shaker flask at 160RPM at 37°C for 16 hours, then inoculate it into a 200L seed fermenter (still the same medium) with 1% inoculum amount, and ventilate at 37°C Cultivate for 16 hours, then transfer to 2000L fermenter according to 10% inoculation amount (still the same culture medium), incubate at 37°C for 20 hours, then raise the temperature to 42°C and cultivate for 10 hours, stop the fermentation after 80% of the bacteria produce spores . Detect the bacterial concentration and reach 10 9 , and then mixed with high-quality peat powder (commercially available) after dry heat st...

Embodiment 2

[0052] Example 2: Using the method provided by the invention to ferment Dr. Ma Xinku No. 1 to prepare microbial strain agent

[0053] Activate the strains first, then rinse the slope with 5ml of sterile water to form a bacterial suspension, and put the bacterial suspension into a 500ml triangular flask containing 100ml of new 1 liquid medium, and use a shaker to cultivate for 18 hours. 37°C, 180-200rpm, detect the bacterial concentration, when the concentration reaches 10 8 Order of magnitude, proceed to the next step;

[0054] Transfer 100ml of bacterial solution from a 500ml conical flask into a 2000ml conical flask, which contains 700ml of new 2 medium, and cultivate it on a shaker for 20 hours at 37°C, 180-200rpm; Concentration up to 10 8 Order of magnitude, proceed to the next step;

[0055] Put the bacterial solution in the 2000ml Erlenmeyer flask into 1m 3 In the fermenter, the intake volume is 6 liters, the fermenter contains 500L of new 3 medium, the fermentatio...

Embodiment 3

[0057] Embodiment 3: Under the condition that does not stockpile for a long time, carry out bacterial count detection to the microbial strain preparation that utilizes traditional method to prepare

[0058] The live bacteria content in the microbial strain agent was detected by the conventional dilution plate counting method. Weigh 1 gram of microbial strain agent (stocked for half a month), dissolve it with sterile water, dilute it with sterile water until the number of bacteria is 20-100 / ml, and then pipette 1ml of the diluted solution into a sterile petri dish , add an appropriate amount of new medium 1 (slant medium), mix well and solidify, incubate upside down in a 37°C incubator for 48 hours, count the colonies on the plate, repeat more than three times, and multiply the average value by the one gram of microbial strains The number of milliliters when the agent is diluted so that the concentration of bacteria is 20-100 / ml is the number of strains per gram of microbial ...

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Abstract

The invention relates to a novel bacillus subtilis which has the collection number of CGMCC (China General Microbiological Culture Collection Center) No.5091, a microorganism strain agent and a microorganism fertilizer which are prepared by using the strain as well as preparation methods thereof. According to the strain provided by the invention, the produced microorganism strain agent has the advantages of higher effective microbial content and longer quality guarantee period.

Description

technical field [0001] The invention relates to a novel bacillus subtilis, microbial strain agent and microbial fertilizer prepared by the strain and their preparation method. Background technique [0002] my country has a large population but little arable land, and the arable land decreases year by year with the growth of population and the development of industry and cities. According to the statistics of relevant departments, the average population of our country increases by 16 million every year, while the cultivated land decreases by 4 million mu every year. In 1950, the population was 550 million, the arable land was 1.65 billion mu, and the per capita arable land was 2.99 mu. In 1980, the per capita arable land was 1.51 mu. In 1990, the per capita arable land dropped to 1.1 mu. Acre level. Therefore, the only feasible way to increase the output of agricultural products in our country is to increase the output per unit area. In order to meet the demand for agricul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08C12N1/20C12R1/125
Inventor 麻林涛王莉
Owner 内蒙古鑫鑫盛达生物科技有限公司
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