Bacillus subtilis, microorganism strain agent and microorganism fertilizer prepared by using bacillus subtilis as well as preparation methods thereof
A technology of Bacillus subtilis and microbial strains, applied in the field of microbial seed agents, can solve the problems of short shelf life, poor product quality, low survival ability of bacterial strains, etc., achieve long shelf life, high effective bacterial count, and solve problems with short shelf life. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0049] Example 1: Using traditional methods to ferment Dr. Ma Xinku No. 1 to prepare microbial strains
[0050] Medium: 3% starch, 1% bean cake powder, 0.1% peptone, 0.2% ammonium sulfate, 0.2% dipotassium hydrogen phosphate, 0.1% yeast powder, 0.01% calcium carbonate, pH7.2-7.4.
[0051] After activating the strain, put it into 1000ml of the above-mentioned medium, and culture it in the Erlenmeyer shaker flask at 160RPM at 37°C for 16 hours, then inoculate it into a 200L seed fermenter (still the same medium) with 1% inoculum amount, and ventilate at 37°C Cultivate for 16 hours, then transfer to 2000L fermenter according to 10% inoculation amount (still the same culture medium), incubate at 37°C for 20 hours, then raise the temperature to 42°C and cultivate for 10 hours, stop the fermentation after 80% of the bacteria produce spores . Detect the bacterial concentration and reach 10 9 , and then mixed with high-quality peat powder (commercially available) after dry heat st...
Embodiment 2
[0052] Example 2: Using the method provided by the invention to ferment Dr. Ma Xinku No. 1 to prepare microbial strain agent
[0053] Activate the strains first, then rinse the slope with 5ml of sterile water to form a bacterial suspension, and put the bacterial suspension into a 500ml triangular flask containing 100ml of new 1 liquid medium, and use a shaker to cultivate for 18 hours. 37°C, 180-200rpm, detect the bacterial concentration, when the concentration reaches 10 8 Order of magnitude, proceed to the next step;
[0054] Transfer 100ml of bacterial solution from a 500ml conical flask into a 2000ml conical flask, which contains 700ml of new 2 medium, and cultivate it on a shaker for 20 hours at 37°C, 180-200rpm; Concentration up to 10 8 Order of magnitude, proceed to the next step;
[0055] Put the bacterial solution in the 2000ml Erlenmeyer flask into 1m 3 In the fermenter, the intake volume is 6 liters, the fermenter contains 500L of new 3 medium, the fermentatio...
Embodiment 3
[0057] Embodiment 3: Under the condition that does not stockpile for a long time, carry out bacterial count detection to the microbial strain preparation that utilizes traditional method to prepare
[0058] The live bacteria content in the microbial strain agent was detected by the conventional dilution plate counting method. Weigh 1 gram of microbial strain agent (stocked for half a month), dissolve it with sterile water, dilute it with sterile water until the number of bacteria is 20-100 / ml, and then pipette 1ml of the diluted solution into a sterile petri dish , add an appropriate amount of new medium 1 (slant medium), mix well and solidify, incubate upside down in a 37°C incubator for 48 hours, count the colonies on the plate, repeat more than three times, and multiply the average value by the one gram of microbial strains The number of milliliters when the agent is diluted so that the concentration of bacteria is 20-100 / ml is the number of strains per gram of microbial ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com