Potassium bacteria and process for producing microorganism manure strain agent using said bacteria
A technology of microbial strains and microbial fertilizers, applied in bacteria, organic fertilizers, etc., can solve the problems of decreased activity of bacteria, decreased survival ability, weakened production performance, etc. Effect
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Embodiment 1
[0046] Embodiment 1: Utilize traditional method to carry out fermentation preparation microbial strain agent to Xin Potassium No. 1
[0047] Medium: 0.5% starch, 0.1% sucrose, 0.2% dipotassium hydrogen phosphate, 0.1% ammonium sulfate, 0.05% magnesium sulfate, 0.001% ferric chloride, 0.01% calcium carbonate, 0.02% yeast extract, pH7.2-7.4.
[0048] After the strain is activated, insert it into 1000ml of the above-mentioned culture medium, and culture it in the Erlenmeyer flask at 160RPM at 37°C for 16 hours, then inoculate it into a 200L seed fermenter (still the same culture medium) according to 1% inoculum size, and ventilate at 37°C Cultivate for 16 hours, then transfer to 2000L fermenter (still the same culture medium) according to 10% inoculation amount, cultivate at 37°C for 20 hours, then raise the temperature to 42°C and cultivate for 10 hours, stop fermentation after 80% of the bacteria produce spores . Detect the bacterial concentration and reach 10 9 , and then mi...
Embodiment 2
[0049] Embodiment 2: Utilize the method provided by the invention to ferment No. 1 new potassium to prepare microbial strain agent
[0050] Activate the strains first, then rinse the slope with 5ml of sterile water to form a bacterial suspension, and put the bacterial suspension into a 500ml triangular flask containing 100ml of new 1 liquid medium, and use a shaker to cultivate for 18 hours. 37°C, 180-200rpm, detect the bacterial concentration, when the concentration reaches 10 8 Order of magnitude, proceed to the next step;
[0051] Transfer 100ml of bacterial solution from a 500ml conical flask into a 2000ml conical flask, which contains 700ml of new 2 medium, and cultivate it on a shaker for 20 hours at 37°C, 180-200rpm; Concentration up to 10 8 Order of magnitude, proceed to the next step;
[0052] Put the bacterial solution in the 2000ml Erlenmeyer flask into 1m 3 In the fermenter, the intake volume is 6 liters, the fermenter contains 500L of new 3 medium, the ferment...
Embodiment 3
[0054] Embodiment 3: Under the condition that does not stockpile for a long time, carry out bacterial count detection to the microbial strain preparation that utilizes traditional method to prepare
[0055] The live bacteria content in the microbial strain agent was detected by the conventional dilution plate counting method. Weigh 1 gram of microbial strain agent (stock for half a month), dissolve it with sterile water, dilute it with sterile water until the number of bacteria is 20-100 / ml, and then pipette 1ml of the diluted solution into a sterile petri dish , add an appropriate amount of new 1 medium (slant medium), mix well and solidify, incubate upside down in a 37°C incubator for 48 hours, count the colonies on the plate, repeat more than three times, and multiply the average value by the one gram of microbial strains The number of milliliters when the agent is diluted so that the cell concentration is 20-100 / ml is the number of strains per gram of the microbial strain ...
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