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Composite microbial preparation for degrading phenolic acid of crop straws and preparation method thereof

A technology of microbial preparation and compound microorganism, which is applied in the field of compound microbial preparation and its preparation, can solve the problems of rising abscisic acid content, affecting fertilizer absorption, inhibiting the growth of seedlings, etc. A wide range of effects

Inactive Publication Date: 2020-09-25
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking the rice-rape rotation as an example, rice straw returning to the field in situ will lead to uneven emergence of rape seedlings and inhibited seedling growth, which will affect fertilizer absorption and other problems.
The main reason for the above problems is that there are allelochemicals-phenolic acids in rice residues or decomposed matter. High concentrations of phenolic acids can cause primary reactions such as plant root cell membrane damage and active oxygen explosion, which further lead to root water and nutrient absorption. The secondary reactions such as the decline of capacity, and finally lead to secondary reactions such as the increase of abscisic acid content, the increase of secondary roots, the decrease of photosynthesis and respiration

Method used

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  • Composite microbial preparation for degrading phenolic acid of crop straws and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A preparation method of a composite microbial preparation for degrading crop straw phenolic acid, which comprises the following steps:

[0054] 1) Preparation of the first microbial preparation,

[0055] ①According to the weight percentage of each component: rapeseed cake 45.0%, peanut stalk 15.0%, Neurospora crassa 3.0%, water 37.0%, select rapeseed cake, peanut stalks, Neurospora crassa and water for use;

[0056] ② Mix the rapeseed cake, peanut stalks, Neurospora crassa with water thoroughly, stack and cover with plastic film, carry out solid fermentation at 25℃~28℃, fermentation for 9 days, turn over 2 times;

[0057] ③After the fermentation is completed, air-dry at room temperature (2~3d) to obtain the first microbial preparation (single microbial preparation);

[0058] 2) Preparation of the second microbial preparation,

[0059] ①According to the weight percentage of each component: rapeseed cake 45.0%, peanut straw 15.0%, trichoderma viride 3.0%, water 37.0%, select rapesee...

Embodiment 2

[0077] A preparation method of a composite microbial preparation for degrading crop straw phenolic acid, which comprises the following steps:

[0078] 1) Preparation of the first microbial preparation,

[0079] ①According to the weight percentage of each component: rapeseed cake 45.0%, peanut stalk 15.0%, Neurospora crassa 3.0%, water 37.0%, select rapeseed cake, peanut stalks, Neurospora crassa and water for use;

[0080] ② Mix the rapeseed cake, peanut stalks, Neurospora crassa with water thoroughly, stack and cover with plastic film, carry out solid fermentation at 25℃~28℃, fermentation for 9 days, turn over 2 times;

[0081] ③After the fermentation is completed, air-dry at room temperature (2~3d) to obtain the first microbial preparation (single microbial preparation);

[0082] 2) Preparation of the second microbial preparation,

[0083] ①According to the weight percentage of each component: rapeseed cake 45.0%, peanut straw 15.0%, trichoderma viride 3.0%, water 37.0%, select rapesee...

Embodiment 3

[0101] A preparation method of a composite microbial preparation for degrading crop straw phenolic acid, which comprises the following steps:

[0102] 1) Preparation of the first microbial preparation,

[0103] ①According to the weight percentage of each component: rapeseed cake 45.0%, peanut stalk 15.0%, Neurospora crassa 3.0%, water 37.0%, select rapeseed cake, peanut stalks, Neurospora crassa and water for use;

[0104] ② Mix the rapeseed cake, peanut stalks, Neurospora crassa with water thoroughly, stack and cover with plastic film, carry out solid fermentation at 25℃~28℃, fermentation for 9 days, turn over 2 times;

[0105] ③After the fermentation is completed, air-dry at room temperature (2~3d) to obtain the first microbial preparation (single microbial preparation);

[0106] 2) Preparation of the second microbial preparation,

[0107] ①According to the weight percentage of each component: rapeseed cake 45.0%, peanut straw 15.0%, trichoderma viride 3.0%, water 37.0%, select rapesee...

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Abstract

The invention relates to a composite microbial preparation for degrading phenolic acid of crop straws and a preparation method thereof. The composite microbial preparation for degrading phenolic acidof crop straws is characterized by being prepared by mixing a first microbial preparation, a second microbial preparation, a third microbial preparation and a fourth microbial preparation; the first microbial preparation, the second microbial preparation, the third microbial preparation and the fourth microbial preparation are selected from one of the four materials according to application; the composite microbial preparation is prepared from the following components in percentage by weight: 40.0-50.0 percent of rape seed cake, 10.0-20.0 percent of peanut straws, 2.0-4.0 percent of neurosporacrassa, trichoderma viride, rhizopus oryzae or aspergillus aculeatus and 35.0-40.0 percent of water. The composite microbial preparation has the advantages of high colony-forming units, environmentalcondition tolerance, long-time preservation at room temperature, small influence by environment after application and low use cost, and can be used for degrading phenolic acid generated by decomposition of crop straws while effectively decomposing crop straws.

Description

Technical field [0001] The invention relates to a composite microbial preparation for degrading phenolic acid of crop straw and a preparation method thereof. Background technique [0002] Returning rice, wheat and other crop stalks to the field is one of the effective ways for the sustainable development of agriculture in our country. It plays a positive role in maintaining the soil nutrient balance. With the improvement of agricultural mechanization, direct return of straw to the field is becoming more and more common. However, the negative effects of returning rice, wheat and other crop straws in situ on the growth of subsequent crop seedlings have not received enough attention. Taking the rice-cole wheel as an example, returning rice straw to the field in situ will cause the rape seedlings to emerge unevenly and the growth of seedlings will be inhibited, which will affect fertilizer absorption and other problems. The main reason for the above problems is the presence of allel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C05F17/20C05G3/40C12R1/885C12R1/845C12R1/66C12R1/645
CPCC05F5/002C05F11/08C12N1/14C05F17/20C05G3/40C05F11/00Y02W30/40
Inventor 廖星李银水秦璐谢立华顾炽明韩配配李小勇胡小加
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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