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Phosphorus bacteria and process for producing microorganism manure strain agent using said bacteria

A technology of microbial strains and microbial fertilizers, which is applied in the direction of bacteria and organic fertilizers, can solve the problems of decreased activity of strains, weakened production performance, and decreased survival ability, and achieves long shelf life, short shelf life, and high effective bacteria count. Effect

Inactive Publication Date: 2006-05-24
河南麻博士喜万家生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The number of effective viable bacteria in microbial fertilizers is an important indicator of product quality. If the number of effective viable bacteria does not meet the standard, the quality of the product cannot be guaranteed. It reflects that the application effect is unstable. The main reason is the problem of strains. After being used as a microbial strain agent, the survival ability during the preservation process is reduced, and the survival time is shortened
In the process of production and use, the activity of the bacteria is reduced, the production performance is weakened, the reproduction speed is slowed down, and the stress resistance is poor.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Utilize traditional method to ferment Xinphos No. 1 to prepare microbial strain agent

[0047] Medium: 3% starch, 1% bean cake powder, 0.1% peptone, 0.2% ammonium sulfate, 0.2% dipotassium hydrogen phosphate, 0.1% yeast powder, 0.01% calcium carbonate, pH7.2-7.4.

[0048] After the strain is activated, insert it into 1000ml of the above-mentioned culture medium, and culture it in the Erlenmeyer flask at 160RPM at 37°C for 16 hours, then inoculate it into a 200L seed fermenter (still the same culture medium) according to 1% inoculum size, and ventilate at 37°C Cultivate for 16 hours, then transfer to 2000L fermenter (still the same culture medium) according to 10% inoculation amount, cultivate at 37°C for 20 hours, then raise the temperature to 42°C and cultivate for 10 hours, stop fermentation after 80% of the bacteria produce spores . Detect the bacterial concentration and reach 10 9 , and then mix it with high-quality peat powder (commercially availabl...

Embodiment 2

[0049] Embodiment 2: Utilize the method provided by the invention to ferment No. 1 new phosphorus to prepare microbial strain agent

[0050] Activate the strain first, then rinse the slope with 5ml of sterile water to form a bacterial suspension, and put the bacterial suspension into a 500ml triangular flask, which contains 100ml of new phosphorus No. 1 liquid medium, and cultivate it on a shaker for 18 hours. The culture conditions are 37°C, 180-200rpm, and the concentration of bacteria is detected, and when the concentration reaches 10 8 Order of magnitude, proceed to the next step;

[0051] Transfer 100ml of bacterial solution from a 500ml conical flask into a 2000ml conical flask, which contains 700ml of new phosphorus No. 2 medium, and cultivate it on a shaker for 20 hours at 37°C, 180-200rpm; detect the bacterial concentration , at concentrations up to 10 8 Order of magnitude, proceed to the next step;

[0052] Take out the bacterial solution from the 2000ml triangula...

Embodiment 3

[0054] Embodiment 3: Under the condition that does not stockpile for a long time, carry out bacterial count detection to the microbial strain preparation that utilizes traditional method to prepare

[0055] The live bacteria content in the microbial strain agent was detected by the conventional dilution plate counting method. Weigh 1 gram of microbial strain agent (stock for half a month), dissolve it with sterile water, dilute it with sterile water until the number of bacteria is 20-100 / ml, and then pipette 1ml of the diluted solution into a sterile petri dish , add an appropriate amount of New Phosphorus No. 1 medium (slant medium), mix well and solidify, incubate upside down in a 37°C incubator for 48 hours, count the colonies on the plate, repeat more than three times, and multiply the average value by the one gram of microorganisms The number of milliliters when the strain agent is diluted so that the cell concentration is 20-100 / ml is the number of strains per gram of th...

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Abstract

The technical scheme of the invention belongs to the field of microbial fertilizer in the field of biological fertilizer. In recent years, microbial fertilizers, according to the national inspection, have a pass rate of less than 60% in spot checks. The main problem with product quality is that the number of effective bacteria does not meet the standard and the shelf life is too short. After the strains are prepared as microbial strain preparations, their survival ability decreases and their survival time becomes shorter during the preservation process. In the process of production and use, the activity of the bacteria is reduced, the production performance is weakened, the reproduction speed is slowed down, and the stress resistance is poor. The problem of bacterial strains has always plagued the development of microbial fertilizers. The new phosphorus strains provided by the invention and the microbial strain agent produced by using the new technology scheme can very well solve the problems of the number of strains reaching the standard and prolonging the shelf life, which makes the invention very important and meaningful.

Description

technical field [0001] The technical solution of the present invention belongs to the field of agricultural fertilizers, specifically, belongs to the field of microbial fertilizers in the field of biological fertilizers, and belongs to the field of C05F 11 / 08 according to the International Patent Classification. Background technique [0002] my country has a large population but little arable land, and the arable land decreases year by year with the growth of population and the development of industry and cities. According to the statistics of relevant departments, the average population of our country increases by 16 million every year, while the cultivated land decreases by 4 million mu every year. In 1950, the population was 550 million, the arable land was 1.65 billion mu, and the per capita arable land was 2.99 mu. In 1980, the per capita arable land was 1.51 mu. In 1990, the per capita arable land dropped to 1.1 mu. Acre level. Therefore, the only feasible way to inc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08
Inventor 麻林涛王莉
Owner 河南麻博士喜万家生物科技有限公司
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