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High performance liquid chromatography (HPLC) fingerprint detection method for blood-nourishing brain-refreshing grain

A technology of blood-nourishing brain granules and fingerprints, which is applied in blood diseases, measuring devices, medical formulas, etc.

Active Publication Date: 2014-12-03
TIANJIN TASLY MORDEN TCM RESOURCES
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AI Technical Summary

Problems solved by technology

At present, the research on the fingerprint of Yangxue Xuenao Granules has not been reported at home and abroad. In order to better control its quality and ensure the safety and effectiveness of clinical medication, this experiment uses the HPLC method to conduct a preliminary study on its fingerprint , on the basis of methodological investigation, the HPLC fingerprint of Yangxue Xuenao Granules was established, and the attribution of the common peaks in the preparation to the medicinal materials was investigated, which provided more comprehensive information for its quality control

Method used

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  • High performance liquid chromatography (HPLC) fingerprint detection method for blood-nourishing brain-refreshing grain
  • High performance liquid chromatography (HPLC) fingerprint detection method for blood-nourishing brain-refreshing grain
  • High performance liquid chromatography (HPLC) fingerprint detection method for blood-nourishing brain-refreshing grain

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Experimental program
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Embodiment 1

[0052] Embodiment 1, detection method

[0053] 1 Instruments and reagents

[0054] Agilent1200 high performance liquid chromatography and Finnigan TSQ Quantum Discovery liquid mass spectrometry system; Milli-Q ultrapure water system (Millipore, France); methanol and acetonitrile are chromatographically pure; reference substances ferulic acid, caffeic acid, rosemary Both acid and baicalin were purchased from China National Institute for the Control of Pharmaceutical and Biological Products; Yangxue Qingnao Granules were provided by Tianjin Tasly Pharmaceutical Co., Ltd.

[0055] 2 Preparation of the solution

[0056] 10 batches of Yangxue Qingnao Granules with qualified standard test solution quality were used as standard products, accurately weighed 0.5g in a 10mL volumetric flask, added 75% methanol to the volume, put it to room temperature after ultrasonication for 20min, and added 75% methanol Make up to the mark, filter through a 0.45 μm microporous membrane, and use it ...

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Abstract

The invention discloses a high performance liquid chromatography (HPLC) fingerprint detection method for blood-nourishing brain-refreshing grains, which is characterized by comprising the following steps of: 1) preparing blood-nourishing brain-refreshing grain test sample soution, precisely weighing the blood-nourishing brain-refreshing grain, and preparing into solution by 3 parts of methyl alcohol and 1 part of water to serve as preparation test sample solution; and 2) injecting the solution obtained in step 1) into a high performance liquid chromatograph for measuring to respectively obtain the chromatogram map of the solution. According to the self characteristics of the blood-nourishing brain-refreshing grain, the fingerprint of the blood-nourishing brain-refreshing grain is measured by the high performance liquid chromatography, an optimized chromatogram condition is found, a measured result is precise, and the receptivity and the stability are good.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an HPLC fingerprint detection method of Yangxueqingnao Granules. Background technique [0002] Yangxue Xuenao Granules are refined from 11 traditional Chinese medicines including Angelica, Chuanxiong, Paeoniae Alba, Uncaria, Millae, Rehmannia, Cassia, Prunella, Asarum, Corydalis and Mother of Pearl, and are refined by modern preparation technology. The function of blood calming the liver, promoting blood circulation and dredging collaterals is used for headache, dizziness, irritability, insomnia and dreaminess caused by blood deficiency and hyperactivity of the liver. It is a traditional Chinese medicine variety under the national key protection. [0003] Among them, ferulic acid, the index component shared by Angelica and Chuanxiong, has been proved by research to have the effects of relaxing blood vessels, increasing serum albumin and preventing lipid peroxidation damage, and ...

Claims

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Application Information

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IPC IPC(8): G01N30/02A61K36/804A61K9/16G01N30/36A61P7/06A61P1/16A61P25/00A61P1/08A61P25/20A61K35/56A61K35/618
Inventor 高钧李文博佟玲倪倩
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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