Alloyed metal colloid
A technology of alloy type and colloid, which is applied in the direction of sol preparation, material inspection products, measuring devices, etc., can solve the problems of increased sensitivity and sufficient sensitivity of the assay method, and achieve high sensitivity and high identification effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0067] The synthesis of embodiment 1 alloy type Au / Pt composite colloid
[0068] Add 1% (w / v) chloroauric acid 18ml to 253.2ml of water, then add 1% (w / v) glycine 18ml, 0.5N KOH 8.1ml as a reducing agent, and then add 1% (w / v) After adding 2.70 mL of potassium chloroplatinate (II) (Pt content: 15%), the reaction was carried out at a temperature of 50° C. for 15 minutes. Then move to a water bath at 98° C. for 30 minutes. After adding 300ml of water, perform ultrafiltration and concentrate to 100ml to prepare Au / Pt composite colloid.
[0069] The colloid obtained by this method presents a blackish-purple color close to black, and when observed through an electron microscope, it is not an Au core Pt shell type but a smooth alloy type (homogeneous alloy) Au / Pt composite colloid in which Au and Pt are integrated. Electron microscope photographs are shown in figure 1 .
Embodiment 2
[0070] Example 2 Preparation of labeled antibody
[0071] Mix 20 μg of anti-human IgG goat antibody with 1 mL (OD 520 =10) were mixed and stirred at room temperature for about 6 hours to bind the antibody to the colloid. The casein solution was added so that the final concentration would be 1%, and the colloidal surface was suppressed by stirring overnight at room temperature. It was collected by centrifugation at 4500g and 4°C for 10 minutes, and suspended in a buffer solution containing 1% casein to prepare an alloy-type Au / Pt complex colloid-labeled antibody.
Embodiment 3
[0090] Au / Pt composite colloids were synthesized by setting the amount of Pt mixed with chloroauric acid to 10%, 12%, 13%, 15%, 18%, and 20%. In 253.2ml of water, add 1% (w / v) chloroauric acid 18ml and respectively with 1.80mL (Pt amount 10%), 2.70mL (Pt amount 15%), 2.16mL (Pt amount 12%), 2.34mL ( 1% (w / v) potassium chloroplatinate (II) was added in an amount of 13% Pt), 2.70 mL (15% Pt), 3.24 mL (18% Pt), and 3.60 mL (20% Pt). Next, after adding 8.1 ml of 0.5 N KOH and 18 ml of 1% (w / v) glycine as a reducing agent, the mixture was reacted at a temperature of 50° C. for 15 minutes. Then move to a water bath at 98° C. for 30 minutes. After adding 300ml of water, perform ultrafiltration and concentrate to 100ml to prepare Au / Pt composite colloids with Pt contents of 10%, 12%, 13%, 15%, 18%, and 20%.
[0091] Using each obtained alloy-type Au / Pt composite colloid, an alloy-type Au / Pt composite-colloid-labeled antibody was prepared in the same manner as in Example 2.
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More