Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic sterile rice and method of propagating core seeds

Abstract

Disclosed are a method of screening nucleic sterile plants of photo-thermo-sensitive nucleic male sterile rice and a method of propagating nucleic seeds. The method of screening nucleic sterile plants of photo-thermo-sensitive nucleic male sterile rice includes: using artificial circulating water at the certain constant temperature within a range of from 22.5 DEG C to 23.5 DEG C to irrigate large-group photo-thermo-sensitive nucleic male sterile rice at a fertility sensitive stage; and screening sterility-stable nucleic sterile plants with zero fertile pollen. The method of propagating nucleic seeds includes: allowing nucleic sterile plants with ears cut off to regenerate and tiller into a fertility sensitive stage; and then using the artificial circulating water at the certain constant temperature within a range of from 19.5 DEG C to 21.5 DEG C to irrigate so as to lead the plants to self and seed to propagate nucleic seeds. Using the methods solves the serious problem that hybrid seed production fails due to the fact that part of sterile plants restores fertility occasionally at low temperature when the critical sterility temperature is too high in hybrid seed production. By propagating the nucleic seeds, the scale of the nucleic plants is widened to meet practical production requirements, and significance on promoting industrial development of 'two-line' hybrid rice is achieved.

Description

technical field [0001] The invention relates to a method for screening core sterile plants of photothermosensitive genic male sterile rice and propagating core seeds. Background technique [0002] In 1981, Shi Mingsong reported that a male sterile plant was found in the 58 population of Rice Nongken. The research proved that the male sterile plant has dual-purpose characteristics, that is, the earing before September 3 is male sterile and can be used as a sterile line. For hybrid seed production, the heading pollen after September 3 is fertile and self-fertile, and is used for breeding CMS seeds. After research, it was named Hubei photosensitive GMS line Nongken 58S. In 1987, under the guidance of Yuan Longping and Li Bihu, Deng Huafeng invented the temperature-sensitive dual-use CMS line Annong S-1. As a result, hybrid rice has developed from a "three-line method" to a "two-line method". However, in the "two-line method" hybrid rice research and application, it is found ...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention has two: one is to provide a kind of method for screening the core sterile plant of photothermosensitive genic male sterile rice, to solve the problem of partial sterile plants in hybrid seed production because the critical sterility temperature is too high, Restoring fertility under low temperature leads to a serious problem of hybrid seed production failure; the second is to provide a method for propagating core seeds, which is used to expand the population of core plants and meet the needs of large-scale production of sterile line seeds