Preparation method of nitrogen oxide donor - quantum dots compound

A technology of nitric oxide and quantum dots is applied in the intersection of chemistry, biology and materials to achieve the effects of good biocompatibility, simple preparation method and simple operation.

Inactive Publication Date: 2012-06-27
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there is no relevant patent report on the preparation of nitric oxide donors by reacti

Method used

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  • Preparation method of nitrogen oxide donor - quantum dots compound
  • Preparation method of nitrogen oxide donor - quantum dots compound
  • Preparation method of nitrogen oxide donor - quantum dots compound

Examples

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Embodiment 1

[0028] Such as figure 1 Shown is a schematic diagram of the preparation process of the nitric oxide donor-quantum dot complex for in-situ detection of nitric oxide release in the present invention. At room temperature, chitosan with a molecular weight of 80,000 and a deacetylation degree of 58% is dissolved in In deionized water, a chitosan aqueous solution with a concentration of 3mg / ml was obtained; under stirring at room temperature, a cadmium chloride aqueous solution with a concentration of 0.005mol / L was added to the chitosan aqueous solution, and stirred for 8 hours to obtain chitosan The cadmium ion complex solution; the resulting solution is placed in a three-necked flask, sealed and protected by nitrogen gas, and an aqueous solution containing sodium selenium hydride equimolar to cadmium chloride is added under high-speed stirring at room temperature to obtain a uniform and transparent colloidal solution , continue to reflux at a temperature of 90°C for 2.5 hours und...

Embodiment 2

[0030] At room temperature, molecular weight is 100,000, the chitosan that deacetylation degree is 60% is dissolved in deionized water, and the obtained concentration is the chitosan aqueous solution of 3.5mg / ml; Add a cadmium acetate aqueous solution with a concentration of 0.005mol / L to the solution, stir for 10 hours, and obtain a cadmium ion complex solution of chitosan; place the resulting solution in a three-necked flask, seal it and protect it with nitrogen, and stir it at room temperature under the condition of high speed Add an aqueous solution of sodium telluride hydride that is equimolar to cadmium acetate to obtain a uniform and transparent colloidal solution, continue to reflux at a temperature of 85°C for 3 hours under nitrogen protection and stirring conditions, and use a molecular weight cut-off of 15,000 after rapid cooling. The dialysis bag was dialyzed in deionized water for 4 days, then concentrated in a rotary evaporator, and then freeze-dried to obtain a s...

Embodiment 3

[0032]At room temperature, molecular weight is 50,000, the chitosan that deacetylation degree is 60% is dissolved in deionized water, and the obtained concentration is the chitosan aqueous solution of 5mg / ml; Add a cadmium acetate aqueous solution with a concentration of 0.0008mol / L, stir for 10 hours, and obtain a cadmium ion complex solution of chitosan; place the resulting solution in a three-necked flask, seal it and protect it with nitrogen, and add it under high-speed stirring at room temperature. An aqueous solution containing sodium telluride hydride which is equimolar to cadmium acetate, to obtain a uniform and transparent colloidal solution, continue to reflux at a temperature of 85°C for 3 hours under nitrogen protection and stirring conditions, and use dialysis with a molecular weight cut-off of 15,000 after rapid cooling The bag was dialyzed in deionized water for 4 days, then concentrated in a rotary evaporator, and then freeze-dried to obtain a stable complex of ...

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Abstract

The invention relates to a nitrogen oxide donor-quantum dots compound and a preparation method thereof. The preparation method comprises the following steps that: chitosan or chitosan derivative with certain deacelation degree is dissolved in the water, metal salt solution and chalcogenide solution with appropriate quantity are sequentially added into the chitosan or the chitosan derivative solution under a room-temperature stirring condition, a compound of chitosan or the chitosan derivative and quantum dots is obtained by heating and backflowing, dialyzing, evaporating and drying the mixture solution, and then the compound is reacted with nitrogen oxide under a pressurizing condition to prepare a nitrogen oxide donor. Compared with the prior art, chitosan is adopted as a carrier for releasing the nitrogen oxide, quantum dots are adopted as a fluorescent probe for detecting the release of the nitrogen oxide, the nitrogen oxide can be stably released under the phycological environment, a function on detecting the release of the nitrogen oxide can be realized, the biocompatibility is good, no physiological toxicity exists, and a promising prospect can be realized on the diagnosis and treatment aspects of hypertension, heat diseases, respiratory diseases, sexual disorder and the like.

Description

technical field [0001] The invention belongs to the interdisciplinary field of chemistry, biology and materials, and in particular relates to a nitric oxide donor capable of releasing nitric oxide and detecting its release and a preparation method thereof. Background technique [0002] Nitric oxide (NO) is a very important biological messenger molecule. The lack of nitric oxide can lead to organ dysfunction, high blood pressure, heart disease, respiratory diseases, etc. In order to treat various diseases caused by nitric oxide deficiency, many kinds of nitric oxide donors have been designed and synthesized. Among them, the nucleophilic nitric oxide donor diazeniumdiolate is a kind of in situ donor with excellent performance developed in recent years. Probing nitric oxide release from nitric oxide donor-quantum dot complexes. This type of nucleophilic nitric oxide donor is generally prepared by reacting a nucleophilic compound containing a secondary amine group with nitric o...

Claims

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Application Information

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IPC IPC(8): C08L5/08C08B37/08C09K11/88C09K11/56G01N33/52C01B21/24
Inventor 万锕俊谭连江李慧丽
Owner SHANGHAI JIAO TONG UNIV
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