Method for identifying resistance to bacterial wilt disease of mulberry

Abstract

The invention discloses a method for identifying resistance to a bacterial wilt disease of mulberry. The method comprises the steps of: inoculating damaged roots into bacterial wilt pathogenic bacteria liquid after planting different resistant varieties or strains of mulberry seedlings with certain seedling age to be identified, a disease-resistance control mulberry seedling and a disease-susceptibility control mulberry seedling indoors by water, controlling epidemic factors, then investigating disease incidence and evaluating the resistance according to a resistance evaluation standard to obtain mulberry varieties and strains with different disease resistances. The method has the advantages of simplicity in operation, accurate and reliable result and good repeatability, and has great application potential in the aspects such as identifying of resistance to bacterial wilt disease of the mulberry, evaluating of a disease-resistant resource, screening of a disease-resistant breeding material and the like.

Description

technical field [0001] The invention relates to a method for identifying the bacterial wilt resistance ability of mulberry trees. Background technique [0002] Bacterial wilt of mulberry is one of the main mulberry diseases threatening sericulture production in China, especially in southern China. Planting mulberry varieties resistant to bacterial wilt is the most fundamental way to prevent and control the disease, but mulberry germplasm resources There are few resistant materials that have been identified and screened for breeding against bacterial wilt, and because mulberry is a perennial woody plant, the evaluation and screening methods for its resistance to bacterial wilt are very different from those of vegetables and other crops. These all seriously restrict the selection and breeding of new varieties of mulberry resistant to bacterial wilt. [0003] At present, the identification and screening of mulberry bacterial wilt resistance materials are mainly carried out thr...

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Problems solved by technology

Natural inoculation identification in the field needs to be carried out on plots with serious diseases and uniform soil texture, fertility, and disease severity. The identification period is long, and it is easily affected by factors such as the external environment, and the results are unstable.

Indoor artificial inoculation mainly adopts methods such as soilless culture large tree suspension inoculation and isolated mulberry branch injection inoculation. The former is labor-intensive, time-consuming and costly to inoculate because mulberry is a perennial woody plant; although the latter is simple to operate, it is insufficient. The disadvantage is that it must rely on a mature in vitro culture system. At present, the in vitro culture of mulberry branches of mulberry trees is restricted by conditions such as variety types. Some varieties of mulberry branches wilt after being isolated, and it is difficult to survive. Ex vivo screening of materials

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