Ammonia (ammonium ion) determination method and ammonia (ammonium ion) diagnosis/determination kit
A technology of ammonia ions and reagents, which is applied in the field of medical/food inspection and determination, and can solve problems such as undiscovered blood ammonia determination methods
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Embodiment 1
[0122] Embodiment 1: the mensuration of ammonia (ammonia ion) content in blood plasma
[0123] 1) Preparation of standard samples
[0124]Dissolve a certain amount of ammonia salt in water or buffer solution, and then adjust the ammonia concentration to 100 micromole / liter;
[0125] 2) Pretreatment of samples to be tested
[0126] Plasma samples are used as samples to be tested without pretreatment;
[0127] 3) Blank sample
[0128] Described water or buffer solution are used as blank sample, and its ammonia concentration is 0 micromole / liter;
[0129] B. Preparation of reagent solution:
[0130] The ammonia diagnosis / measurement reagent of the present embodiment is a single-dose reagent, which contains:
[0131] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0132] Glycerin 1mol / L
[0133] NADP + 0.25mmol / L
[0134] Ammonia kinase 16000U / L
[0135] Ketokinase 18000U / L
[0136] Fructose dehydrogenase 12000U / L
[0137] Aden...
Embodiment 2
[0151] Embodiment 2: the mensuration of ammonia (ammonia ion) content in blood plasma
[0152] 1) Preparation of standard samples
[0153] A certain amount of ammonia salt is dissolved in water, and then the concentration of ammonia (ammonia ion) is adjusted to 100 micromole / liter, as a standard sample;
[0154] 2) Preparation of samples to be tested
[0155] Plasma samples are used as samples to be tested without pretreatment;
[0156] 3) Blank sample
[0157] Described water is used as blank sample, and its ammonia (ammonia ion) concentration is 0 micromole / liter;
[0158] B. Preparation of reagent solution:
[0159] Ammonia (ammonia ion) diagnostic / determination reagent is a two-dose reagent, which contains:
[0160] Reagent 1 with the following composition:
[0161] Phosphate buffer 100mmol / L
[0162] NAD + 0.25mmol / L
[0163] Adenosine triphosphate 5mmol / L
[0164] Fructose 1-phosphate 5mmol / L;
[0165] Reagent 2 with the following composition...
Embodiment 3
[0187] Embodiment 3: the mensuration of ammonia (ammonia ion) content in plasma sample
[0188] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:
[0189] B. Preparation of reagent solution:
[0190] Ammonia (ammonia ion) diagnostic / assay reagent is a three-dose reagent that contains:
[0191] Reagent 1 with the following composition:
[0192] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0193] NAD + 0.25mmol / L
[0194] Adenosine triphosphate 6mmol / L
[0195] Fructose 1-phosphate 4mmol / L;
[0196] Reagent 2 with the following composition:
[0197] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0198] Sodium chloride 2mol / L
[0199] Ketokinase 28000U / L
[0200] Fructose dehydrogenase 50000U / L;
[0201] Reagent 3 with the following composition:
[0202] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
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