Solid-state fermentation method for tobacco stems and solid-state fermentation device for tobacco stems

A solid-state fermentation and tobacco stem technology, applied in tobacco, tobacco processing, application, etc., can solve problems such as unfavorable solid-state fermentation of tobacco stems, inability to apply tobacco stem solid-state fermentation, and unsuitable tobacco stem materials

Active Publication Date: 2012-07-18
SHANGHAI TOBACCO GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But many inherent modes cannot be applied to solid-state fermentation of tobacco stems
For example, the water immersion operation of fermented raw materials is generally to soak the raw materials in water to make the raw materials fully absorb water, but this is not suitable for tobacco

Method used

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  • Solid-state fermentation method for tobacco stems and solid-state fermentation device for tobacco stems
  • Solid-state fermentation method for tobacco stems and solid-state fermentation device for tobacco stems
  • Solid-state fermentation method for tobacco stems and solid-state fermentation device for tobacco stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1150L

[0062] Example 1 Solid-state fermentation of tobacco stems in a 1150L solid-state fermentation vessel—upright stacking of culture frames

[0063] 1. Preparation of Aspergillus niger Seed Solution

[0064] 1) Incline cultivation

[0065] Aspergillus niger (Aspergillus niger, 40431-Aspergillus niger preserved and provided by China Center of Industrial Culture Collection) was streak-inoculated on a PDA slant, cultured at 30°C for 5 days, and stored in a refrigerator at 4°C.

[0066] 2) Preparation of Aspergillus niger spore suspension

[0067] Add a certain amount of sterile water sterilized in advance to the Aspergillus niger PDA slant, scrape off the Aspergillus niger spores with an inoculation shovel, transfer them to a sterilized Erlenmeyer flask, vibrate and break up the glass beads to obtain the Aspergillus niger spore suspension; The concentration of spores is 10 7 pieces / ml.

[0068] 3) Preparation of Aspergillus niger seed solution

[0069] Add 25g of tobacco leaf p...

Embodiment 2150L

[0080] Example 2 Solid-state fermentation of tobacco stems in a 150L solid-state fermentation vessel—staggered stacking of culture frames

[0081] 1. Preparation of Aspergillus niger Seed Solution

[0082] 1) Incline cultivation

[0083] Aspergillus niger (Aspergillus niger, 40431-Aspergillus niger preserved and provided by China Center of Industrial Culture Collection) was streaked and inoculated on a PDA slant, cultured at 30°C for 5 days, and stored in a refrigerator at 4°C .

[0084] 2) Preparation of Aspergillus niger spore suspension

[0085] Add a certain amount of sterile water sterilized in advance to the Aspergillus niger PDA slant, scrape off the Aspergillus niger spores with an inoculation shovel, transfer them to a sterilized Erlenmeyer flask, vibrate and break up the glass beads to obtain the Aspergillus niger spore suspension; The concentration of spores is 10 7 pieces / ml.

[0086] 3) Preparation of Aspergillus niger seed solution

[0087] Add 25g of tobac...

Embodiment 3

[0098] Example 3 Solid-state fermentation of tobacco stems in the fermentation chamber—upright stacking of culture frames

[0099] 1. Preparation of Aspergillus niger Seed Solution

[0100] 1) Incline cultivation

[0101] Aspergillus niger (Aspergillus niger, 40431-Aspergillus niger preserved and provided by China Center of Industrial Culture Collection) was streak-inoculated on a PDA slant, cultured at 30°C for 5 days, and stored in a refrigerator at 4°C.

[0102] 2) Preparation of Aspergillus niger spore suspension and shake flask culture

[0103] Add a certain amount of sterile water sterilized in advance to the Aspergillus niger PDA slant, scrape off the Aspergillus niger spores with an inoculation shovel, transfer them to a sterilized Erlenmeyer flask, vibrate and break up the glass beads to obtain the Aspergillus niger spore suspension; The concentration of spores is 10 7 pieces / ml.

[0104] Add 25g of tobacco leaf powder (dry basis) through a 36-mesh sieve to each o...

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Abstract

The invention relates to the technical field of tobacco processing, and discloses a new system suitable for solid-state fermentation of tobacco stems and a corresponding solid-state tobacco stem fermentation method. Specifically, the tobacco stems are added in a drum tank, a certain quantity of hot water is added in the drum tank while the drum tank rotates, and the tobacco stems absorb water for 30 minutes; after being cooled down to room temperature, the tobacco stems containing water are added in another drum tank, and a certain quantity of microbial seed liquid is added into the other drum tank to realize microbial inoculation while the other drum tank rotate; and after inoculation, the tobacco stems are fed in cultivating frames, the cultivating frames are stacked on a movable storage cage in a certain mode so as to vibrate and increase feeding rate, the storage cage is placed in a fermentation chamber or a fermentation container to realize solid-state fermentation, ventilation is carried out according to needs, and after fermentation is finished, microbial spore is sucked. The new system suitable for solid-state fermentation of tobacco stems and the corresponding solid-state tobacco stem fermentation method can increase mechanical level and automatic level of a production process of solid-state fermentation of the tobacco stems, increase spatial feeding coefficients of solid-state fermentation materials, and accordingly improve production efficiency of solid-state fermentation of the tobacco stems.

Description

technical field [0001] The invention relates to the technical field of tobacco processing, in particular to a method for solid-state fermentation of tobacco stems and a new solid-state fermentation system. Background technique [0002] Tobacco stems are the thick and hard veins of tobacco leaves, accounting for about 25% to 30% of the leaf weight. Leaf stems are gradually separated during tobacco processing. Its shape is long strips of different thickness and length. It is often cut into shredded stems in cigarette production, and used in cigarette processing in a certain proportion with shredded tobacco. But the tobacco stem that also has cannot be fully utilized. The chemical composition of tobacco stem is complex, mainly including cellulose, hemicellulose, pectin and so on. The cellulose content accounts for about 20%. Tobacco stems can be processed into tobacco stem fibers, tobacco stem fermentation extracts, etc. in the cigarette industry through microbial fermenta...

Claims

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Application Information

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IPC IPC(8): A24B15/20
Inventor 汤朝起许赣荣于兴伟张晨盛科
Owner SHANGHAI TOBACCO GRP CO LTD
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