Silk fibroin micro-needle system, silk fibroin nanometer particle and preparation method thereof
A technology of microneedle and silk fibroin, which is applied in the field of medical microneedle system, can solve the problems of ineffective reach, poor mechanical strength, human hazards to be studied, etc., and achieve the effect of overcoming harmful side effects
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[0026] The present invention will be further described below in conjunction with accompanying drawing:
[0027] The preparation method of dissolvable silk fibroin microneedle system, refer to figure 1 , including the following main steps:
[0028] Step 1, extracting and preparing a silk fibroin high molecular protein solution from the material of the organism; the extraction method of the high molecular silk fibroin protein solution varies with the starting materials. Biological substances include silk, spider silk, etc. Taking silkworm cocoons as an example, cut the silkworm cocoons into pieces, rinse them with deionized water, put them in a constant temperature water bath, heat and boil them in 20mM sodium carbonate solution for 30 minutes, and repeat the boiling for 30 minutes after changing the liquid, then take out the degummed silk cocoons for use. Rinse with deionized water multiple times, then dry in a drying oven. The dried degummed silkworm cocoons were dissolved ...
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