Method for quickly detecting content of amaranth and sunset yellow in foods

A technology of sunset yellow and amaranth, which is applied in the field of food safety additive detection, achieves the effect of simple operation method, lower detection limit and obvious results

Inactive Publication Date: 2012-08-22
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the extraction and separation of food pigments using two-phase systems, and the rare reports are also based on polymer-salt systems.

Method used

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  • Method for quickly detecting content of amaranth and sunset yellow in foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Utilize the inventive method to detect the content of sunset yellow and amaranth in eye-catching grape-flavored beverage, concrete steps are as follows:

[0022] 1. Making the working curve: take 3.0ml of amaranth standard solution of 1.00, 2.50, 5.00, 20.00, 30.00μg / ml, put it into a 10ml colorimetric tube, dilute to the mark with distilled water, and measure the absorbance at a wavelength of 520nm; take 1.00, 3.00, 5.00, 7.00, 9.00μg / mL Sunset Yellow 3.0ml, put it into a 10ml colorimetric tube, dilute to the mark with distilled water, measure the absorbance at a wavelength of 440nm; make a working curve, get the regression equation, molar absorptivity, correlation See Table 1 for standard deviation, recovery rate, etc.

[0023]

[0024] Table 1: Linear regression equations and associated results for pigments

[0025]

[0026] 2. Sample processing

[0027] Take 1.00ml of eye-catching grape-flavored beverage in a 10ml stoppered centrifuge tube, heat...

Embodiment 2

[0029] Example 2: Utilize the inventive method to detect the content of sunset yellow in the carbonated drink, concrete steps are as follows:

[0030] 1. Working curve creation: take 1.00, 3.00, 5.00, 7.00, 9.00 μg / mL sunset yellow 1.0ml, put it into a 10ml colorimetric tube, dilute to the mark with distilled water, and measure the absorbance at a wavelength of 440nm; the linear regression equation is obtained as A=0.00326C(mg / ml)-0.027 (where C is the absorbance), the molar absorptivity coefficient is 3.0×10 4 L / mol cm.

[0031] 2. Sonicate the carbonated drink for 20 minutes to fully remove the carbon dioxide, take 1.00ml of the drink into a 10ml stoppered centrifuge tube, add 1.5ml of isopropanol and 1.0g (NH 4 ) 2 SO 4 , dilute to the mark with distilled water, shake well, centrifuge at 3500r / min for 10 min, remove the lower solution with a pipette, take the upper organic phase and measure the absorbance at a wavelength of 440nm, and calculate the sunset in the carbo...

Embodiment 3

[0033] Example 3: Utilize the inventive method to detect the content of sunset yellow and amaranth in egg rolls, concrete steps are as follows:

[0034] 1. Working curve making: the method is the same as the method in step 1 of embodiment 1.

[0035] 2. Take 1.000g egg roll, use 6ml of absolute ethanol and ammonia water (volume ratio 1:1) mixture to extract the pigment in two times, combine the extracts, put them in a 10ml stoppered centrifuge tube, add 2.0mL ethanol and 1.2 g Na 2 SO 4 , dilute to the mark with distilled water, shake well, centrifuge at 2000r / min for 15min, remove the lower solution with a pipette, take the upper organic phase and measure the absorbance at 520nm and 440nm wavelength respectively, and calculate the egg roll according to the working curve obtained in step 1 The contents of sunset yellow and amaranth were 0.00052mg / ml and 0.0048mg / ml respectively.

[0036]

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Abstract

The invention discloses a method for quickly detecting the content of amaranth and sunset yellow in foods, which comprises the following steps of: extracting pigments in the foods by utilizing an organic solvent-salt aqueous two-phase system, so that the amaranth and the sunset yellow are extracted into an organic phase for phase separation; and determining the content of the amaranth and the sunset yellow in the organic phase by using an ultraviolet-visible spectrophotometry. The limit of detection in the method is 0.05 mug/mL. The method has the beneficial effects that while the pigments are enriched, the matrix interference is effectively separated; and moreover, the extraction conditions are mild, the operation is simple, the determination speed is quick, effective and accurate and the like.

Description

technical field [0001] The invention relates to a method for quickly detecting the content of amaranth and sunset yellow in food, which belongs to the detection technology of food safety additives. Background technique [0002] Before the middle of the 19th century, people used edible natural pigments for coloring. Since the first synthetic organic pigments were invented by British W.H.PERKINS in 1856, many organic pigments have been synthesized one after another. Due to the bright color, stable property and low cost of this kind of pigment, it quickly replaced the natural pigment. With the development of science, people have gradually discovered the harm of synthetic pigments to the human body. Both sunset yellow and amaranth are azo pigments, which are edible synthetic pigments allowed in my country. Because they can maintain and improve the color, aroma, and taste of food, they generally have bright color, strong coloring power, high fastness, and properties. The advanta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
Inventor 杨亚玲廉源沛刘谋盛
Owner KUNMING UNIV OF SCI & TECH
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