Compound feed addictive with bacteria and oxidation resisting functions
A compound feed and additive technology, which is applied in animal feed, animal feed, additional food elements, etc., can solve the problems of antibiotic resistance enhancement, human health impact, quality decline, etc., to reduce usage, improve feed utilization efficiency, and improve quality Effect
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Embodiment 1
[0055] Embodiment 1 (feed additive product)
[0056] The invention provides a compound feed additive with antibacterial and antioxidative functions;
[0057] The composition of the compound feed additive is as follows:
[0058] 10% antimicrobial peptide, 15% propolis, 15% Ligustrum lucidum extract, 20% freeze-dried bacterial powder of Bacillus subtilis, 40% solid fermentation culture of Ganoderma lucidum; Bacillus subtilis is CGMCC6012, and the above ratio is the quality percentage.
[0059] Antimicrobial peptides are products sold by Shanghai Tricapeptide Biotechnology Co., Ltd.
[0060] The preparation method of ganoderma lucidum solid fermentation culture is as follows:
[0061] Activation culture of slant strains: transfer the preserved ganoderma lucidum slant strains to the slant medium, and cultivate for about 110 hours at 25°C until the mycelia cover the slope;
[0062] 1. Liquid first-level seed culture: pick a piece of the above-mentioned Ganoderma lucidum slant s...
Embodiment 2
[0073] The invention provides a compound feed additive with antibacterial and antioxidative functions;
[0074] The composition is as follows:
[0075] Antimicrobial peptide 15%, propolis 10%, Ligustrum lucidum extract 25%, Bacillus subtilis freeze-dried bacterial powder 10%, Ganoderma lucidum solid fermentation culture 40%; Bacillus subtilis is CGMCC6012, the above ratio is the quality percentage.
[0076] The preparation method of ganoderma lucidum solid fermentation culture is as follows:
[0077] Activation culture of slant strains: transfer the preserved ganoderma lucidum slant strains to the slant medium, and cultivate at 26°C for about 110 hours until the mycelia cover the slope;
[0078] 1. Liquid first-level seed culture: pick a piece of the above-mentioned Ganoderma lucidum slant strain and insert it into a 500-ml shaker flask with 100 ml of medium for first-level seed culture. The culture conditions: rotary shaker 100 rpm, 26 Cultivate for about 100 hours at ℃; c...
Embodiment 3
[0087] 10% antimicrobial peptide, 10% propolis, 10% Ligustrum lucidum extract, 10% freeze-dried bacterial powder of Bacillus subtilis, 60% solid fermentation culture of Ganoderma lucidum; Bacillus subtilis is CGMCC6012, and the above ratios are for mass percentage.
[0088] The preparation method of ganoderma lucidum solid fermentation culture is as follows:
[0089]Activation culture of slant strains: transfer the preserved slant strains of Ganoderma lucidum to the slant medium, and cultivate for about 120 hours at 22°C until the mycelia cover the slant; the optimum culture temperature is 25°C.
[0090] 1. Liquid first-level seed culture: pick a piece of the above-mentioned Ganoderma lucidum slant strain and insert it into a 500-milliliter shaker flask with 100 milliliters of medium for first-level seed culture. Cultivate for about 130 hours;
[0091] 2. Liquid secondary seed culture: Put the seeds of the primary shaker flask with 5% inoculum into a 500 ml shaker flask with...
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