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Method for preparing streptomyces rimosus spores

A technology of Streptomyces fissures and spores is applied in the field of culture and preparation of strains produced by oxytetracycline fermentation. Yield, the effect of increasing body surface area

Active Publication Date: 2013-11-06
内蒙古圣雪大成制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This has to frequently change the spore batch number in production, often resulting in unstable production, and also increases the workload of strain spore preparation

Method used

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  • Method for preparing streptomyces rimosus spores
  • Method for preparing streptomyces rimosus spores
  • Method for preparing streptomyces rimosus spores

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Select wheat grains with plump grains, no insects, no blackheads, and uniform color, and peel them on a peeling machine, remove cracked and broken grains, and dry them in the air. If necessary, use a fan to dry them to prevent mildew and rancidity.

[0030] Take tap water and soak the peeled wheat grains at room temperature (about 26°C) for about 3 hours (only about 1 / 3 of the surface layer of the soaked wheat grains will become soft when squeezed by hand, and the core of the wheat grains is hard, and it will crack in half when bitten with teeth , no sticking). Drain the water to make wet granules (after draining the water, put it on kraft paper, and keep weighing until the draft reaches 50% is better).

[0031] Add 40 meshes of 2% wheat bran powder to the soaked wheat grains, shake gently and fully to make it evenly wrapped on the surface of the wet wheat grains, according to 30 g / eggplant-shaped bottle or 10 g / large test tube The filling volume is divided into subpac...

Embodiment 2

[0038] Choose millet with neat and uniform particles and moderate size, and keep it refrigerated for later use.

[0039] Add 5 g of millet to each tube and add 10 mL of tap water, add 15 g of millet in an eggplant bottle, add 30 mL of tap water, cover the stopper, wrap it with double gauze, and carry out disinfection.

[0040] Sterilize at 121±1°C (0.11±0.05 MPa) for 30 minutes, take it out, cool it to about 35°C, and crush it.

[0041]Use an inoculation needle to dig about 2 mg of spores from 122# sand and inoculate them on a large tube of bran slant medium. The composition of the bran slant is: in terms of mass percentage, 10% bran, 1% agar, and the balance is tap water. The inoculated slant of the large tube was cultured in a 38°C constant temperature room for 4 days, and then transferred to a 31°C constant temperature room for 1-2 days until the spores of Streptomyces chachis matured. Add 10 mL of sterile water to the slant of large tube spores of 122# Streptomyces fissur...

Embodiment 3

[0044] Select barley grains with full grains, no insects, no blackheads, and uniform color, and peel them on the peeling machine, remove cracked and broken grains, and dry them in the air. If necessary, use a fan to dry them to prevent mildew and rancidity.

[0045] According to the weight ratio of 1:3, take tap water and soak the peeled wheat grains at room temperature (about 26°C) for about 3 hours. When the soaked barley grains are pinched by hand, only about 1 / 2 of the surface layer becomes soft, and the wheat core is relatively hard. It will split in two when bitten with your teeth, and it will not stick together. After draining, put it on kraft paper, and keep weighing until the draft reaches 45%~50%.

[0046] Add 40 meshes of 3% wheat bran powder to the soaked wheat grains, shake gently and fully to make it evenly wrapped on the surface of the wet wheat grains, according to 30 g / eggplant-shaped bottle or 10 g / large test tube The filling volume is subpackaged, sterilize...

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Abstract

The invention discloses a method for preparing streptomyces rimosus spores. The method comprises the following steps: (a) taking grains as a culture carrier, soaking the grains by using water or a dilute nutrient solution at room temperature for 2 to 6 hours, and draining water to obtain wet grains; and (b) coating bran powder on the outer layers of the wet grains, sterilizing by using high pressure steam, cooling, inoculating oxytetracycline production strain streptomyces rimosus spore suspension, and culturing until streptomyces rimosus spores are mature. By the method, the batch yield of the streptomyces rimosus spores can be greatly improved, and the preservation period of the streptomyces rimosus spores can be greatly prolonged.

Description

technical field [0001] The invention relates to a method for cultivating and preparing strains produced by fermentation of oxytetracycline, in particular to a method for preparing spores of Streptomyces chamoyces. technical background [0002] Spore preparation is an indispensable link in fermentation industrial production, and the quality of spores will directly affect the success of fermentation production. The spores of Streptomyces chamoyces used in the fermentative production of oxytetracycline have always been prepared on agar slant. The types of slopes are divided into test tube slopes and flat bottle slopes according to the different containers used. The latter is more widely used in production because of the large surface area of ​​the slope. But this slant spore is limited by the plane surface area, and the spore quantity that grows is difficult to meet the requirement of production. For each large test tube, there are at most 3 to 4 billion spores, and each flat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12R1/59
Inventor 郑万静齐巧娟赵丽红徐亲民
Owner 内蒙古圣雪大成制药有限公司
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