Method for producing probiotic bacteria granular feed by fermenting corn straw with mixed strain
A technology for fermenting corn stalks and pellet feed, applied in animal feed, animal feed, applications, etc., can solve the problem that product characteristics and production methods are not suitable for large-scale industrial production, can not solve the problem of straw feed storage and transportation, and low nutrient absorption rate To achieve the effect of increasing the growth rate of animals, facilitating storage and transportation, and increasing the crude protein content
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Embodiment 1
[0025] Step 1. Treatment of corn stalks: take freshly harvested dry corn stalks and crush them to 1 mm;
[0026] Step 2, preparation of fermentation strain
[0027] (1) Preparation of culture medium
[0028] The strain culture medium of Aspergillus niger and Saccharomyces cerevisiae is potato dextrose agar medium, and its specific preparation method is, get 200 grams of potatoes, cut into small pieces, add 1000 milliliters of water and boil for 30 minutes, filter with gauze after cooling to remove potato pieces, add to Add 20 grams of glucose to the filtrate and stir until completely dissolved, add water to 1000 ml, and sterilize at 121°C for 20 minutes; 50ml of water, fully dissolved, dilute to 1000ml with water, and sterilize at 121°C for 20 minutes.
[0029] (2) Preparation of strains
[0030] a. Preparation of Aspergillus niger strains: From the preserved slant strains, use an inoculation loop to scrape 2 rings of Aspergillus niger mycelia and inoculate them in a 500ml ...
Embodiment 2
[0040] Step 1. Treatment of corn stalks: take freshly harvested dry corn stalks and crush them to 5mm;
[0041] Step 2, preparation of fermentation strain
[0042] (1) Preparation of culture medium
[0043] The strain culture medium of Aspergillus niger and Saccharomyces cerevisiae is potato dextrose agar medium, and its specific preparation method is, get 200 grams of potatoes, cut into small pieces, add 1000 milliliters of water and boil for 30 minutes, filter with gauze after cooling to remove potato pieces, add to Add 20 grams of glucose to the filtrate and stir until completely dissolved, add water to 1000 ml, and sterilize at 121°C for 20 minutes; 100ml of water, after fully dissolved, add water to make up to 1000ml, and sterilize at 121°C for 20 minutes.
[0044] (2) Preparation of strains
[0045] a. Preparation of Aspergillus niger strains: From the preserved slant strains, use an inoculation loop to scrape 3 rings of Aspergillus niger mycelia and inoculate them in a...
Embodiment 3
[0055] Step 1. Treatment of corn stalks: take freshly harvested dry corn stalks and crush them to 3mm;
[0056] Step 2, preparation of fermentation strain
[0057] (1) Preparation of culture medium
[0058] The strain culture medium of Aspergillus niger and Saccharomyces cerevisiae is potato dextrose agar medium, and its specific preparation method is, get 200 grams of potatoes, cut into small pieces, add 1000 milliliters of water and boil for 30 minutes, filter with gauze after cooling to remove potato pieces, add to Add 20 grams of glucose to the filtrate and stir until completely dissolved, add water to 1000 ml, and sterilize at 121°C for 20 minutes; 80ml of water, fully dissolved, dilute to 1000ml with water, and sterilize at 121°C for 20 minutes.
[0059] (2) Preparation of strains
[0060] a. Preparation of Aspergillus niger bacterial classification: From the bacterial classification of the slant of preservation, scrape 2-3 ring Aspergillus niger mycelia with inoculat...
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