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In-vitro preparation method of transfer factors capable of resisting duck viral hepatitis

A technology for duck viral hepatitis and transfer factor, applied in the field of immunology, can solve the problems of inability to play a therapeutic role, limited curative effect, poor pertinence, etc., and achieve the effects of low cost, low production cost and manpower saving.

Inactive Publication Date: 2012-10-03
河南后羿生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are some medicines and preparations against duck viral hepatitis at present, because the existing medicine preparations are not highly targeted, the curative effect is limited, and most of the therapeutic preparations are applied after the onset of the disease, so they cannot provide fundamental treatment. effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method for in vitro anti-duck viral hepatitis transfer factor, the present embodiment uses chicken peripheral blood as the basic raw material to prepare monolayer lymphocytes, and induces and cultures monolayer lymphocytes through phytohemagglutinin and duck hepatitis virus, thereby promoting To produce a large amount of anti-duck hepatitis virus specific transfer factor in vitro, the specific steps are as follows:

[0019] 1. Collection of anticoagulant blood: Select healthy chickens without infectious diseases, sterilize the blood collection site, use a 50mL sterile syringe to collect anticoagulant blood from the heart, and add 5mL of heparin sodium with a concentration of 1mg / mL;

[0020] 2. Lymphocyte separation: Add anticoagulant blood and lymphocyte separation solution (purchased from Beijing Puli Biological Co., Ltd., and operate according to the instructions) into the sterilized centrifuge tube at a ratio of 1:1, and add the lymphocyte separation so...

Embodiment 2

[0026] A preparation method for in vitro anti-duck viral hepatitis transfer factor, the present embodiment uses chicken peripheral blood as the basic raw material to prepare monolayer lymphocytes, and induces and cultures monolayer lymphocytes through phytohemagglutinin and duck hepatitis virus, thereby promoting To produce a large amount of anti-duck hepatitis virus specific transfer factor in vitro, the specific steps are as follows:

[0027] 1. Collection of anticoagulant blood: Select healthy chickens without infectious diseases, sterilize the blood collection site, use a 50mL sterile syringe to collect anticoagulant blood from the heart, and add 5mL of heparin sodium with a concentration of 1mg / mL;

[0028] 2. Lymphocyte separation: Add anticoagulant blood and lymphocyte separation solution (purchased from Beijing Puli Biological Co., Ltd., and operate according to the instructions) into the sterilized centrifuge tube at a ratio of 1:1, and add the lymphocyte separation so...

Embodiment 3

[0034] An in vitro preparation method of anti-duck viral hepatitis transfer factor, the present embodiment mainly includes the steps of preparing monolayer lymphocytes from chicken spleen, adding phytohemagglutinin and virus induction, and the specific steps are as follows:

[0035] 1. Spleen collection: select healthy chickens without infectious diseases, remove the spleen under aseptic conditions, then wash the blood on the surface and the liquid on the surface of the internal organs with sterilized saline, disinfect the surface with alcohol, and remove the fat tissue and For fascia, use sterile ophthalmic scissors and ophthalmic tweezers to take part of the tissue from the inside of the spleen, put it in a 30mL beaker, then cut it into pieces with a diameter of less than 0.1cm, and wash it three times with pH7.2 Hanks solution Blood and impurities in the tissue, keep the tissue as clean as possible;

[0036] 2. Splenocyte preparation: add 5 times the amount of trypsin s...

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Abstract

The invention relates to an in-vitro preparation method of transfer factors capable of resisting duck viral hepatitis. In the method, the transfer factors capable of resisting the duck viral hepatitis are prepared through the induction of duck hepatitis virus by utilizing an in-vitro lymphocyte cell culture method; and chicken spleen or peripheral blood is used as a basic raw material to prepare single-layer lymphocyte cells, and then the single-layer lymphocyte cells are induced and cultured through phytohemagglutinin and the duck hepatitis virus, so that a large number of specific transfer factors capable of resisting the duck hepatitis virus are generated in vitro.. The transfer factors can effectively inhibit the virus of duck viral hepatitis, so that the normal bodies cannot be infected with the duck viral hepatitis, and the radical pre-prevention action can be realized. The method provided by the invention has the characteristics of being simple and easy to operate and the like.

Description

technical field [0001] The invention relates to a preparation method of in vitro anti-duck viral hepatitis transfer factor, which belongs to the field of immunology. Background technique [0002] Duck viral hepatitis is an acute and highly fatal infectious disease of ducklings caused by duck hepatitis virus (DVH). This disease is characterized by acute onset, short course of disease, rapid infection, and high morbidity and mortality. , causing significant economic losses to the farming industry. [0003] Transfer factor (TF, Transfer Factor), also known as transfer factor, can be used as a cellular immunity enhancer. It is a low-molecular-weight polypeptide-nucleotide complex released by T lymphocytes that can transfer sensitization information. A certain specific cellular immune function is specifically transferred to the recipient's normal lymphocytes, participates in the body's immune response, and improves the recipient's cellular immune function; at the same time, it p...

Claims

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Application Information

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IPC IPC(8): A61K35/14A61K35/28A61K38/00A61P37/04A61P31/14A61K35/57
Inventor 吴红云郭俊清
Owner 河南后羿生物工程股份有限公司
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