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Extracellular matrix support material and preparation method thereof

A technology of scaffold material and extracellular matrix, applied in the field of extracellular matrix scaffold material and its preparation, based on bone marrow mesenchymal stem cell-derived extracellular matrix scaffold material and its preparation field, which can solve the loss of collagen and growth factors of amino protein sugar , reduce the biological function of the extracellular matrix scaffold, and achieve the effect of wide tissue source and strong proliferation ability

Inactive Publication Date: 2014-04-09
金成哲 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein denaturant and co-solvent SDS used in the decellularization process will not only break the normal tissue structure, lose the amino protein sugar and destroy the collagen, but also lose a large amount of growth factors (up to 39-72%), thereby reducing the cell growth rate. Biological functions of extracellular matrix (ECM) scaffolds

Method used

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  • Extracellular matrix support material and preparation method thereof
  • Extracellular matrix support material and preparation method thereof
  • Extracellular matrix support material and preparation method thereof

Examples

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Embodiment 1

[0032] (1) Schematic flow chart for preparation of bone marrow mesenchymal stem cell extramatrix scaffold figure 1 , the specific method is as follows:

[0033] (1) Whole bone marrow culture method for primary culture of young rabbit bone marrow mesenchymal stem cells: take fresh 3-week-old young rabbits just slaughtered in the slaughterhouse, separate the left and right femurs and tibia, put them in ice bags and transport them back to the laboratory for the following operations : Soak the femur and tibia in povidone iodine for disinfection, and place them in a petri dish with sterile saline. In the ultra-clean bench, the bone marrow cavity at both ends of the femur and tibia was opened, and the bone marrow cavity was flushed with normal saline until the bone marrow cavity turned white. The bone marrow flushing fluid was collected, centrifuged at 1000 rpm for 10 minutes, the supernatant was discarded, the cell pellet was suspended in DMEM medium, and counted. Take 1×10 8 Ce...

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Abstract

The invention belongs to the field of biological materials and discloses an extracellular matrix support material and a preparation method thereof. The extracellular matrix support material disclosed by the invention is prepared into a three-dimensional porous support material with a certain pore diameter and porosity through performing in-vitro monolayer high-density culture on immature rabbit bone marrow mesenchymal stem cells and collecting extracellular matrixes secreted by the immature rabbit bone marrow mesenchymal stem cells by applying the freeze-drying technology and a proper cross-linking agent. The material can be trimmed into proper sizes and shapes according to a cartilage defect zone or the requirement of an in-vitro experiment, and the material is sterilized by ethylene oxide to obtain an integral packaging bag. As the stem cells are used as material sources of an extracellular matrix support, the extracellular matrix support material disclosed by the invention is wide in tissue source and strong in multiplication capacity, therefore, the extracellular matrix support material can obtain enough cell quantity per se, and the problems of social ethics involved by embryonic stem cells are avoided.

Description

technical field [0001] The invention belongs to the field of biological materials, and relates to an extracellular matrix scaffolding material and a preparation method thereof, in particular to an extracellular matrix scaffolding material based on bone marrow mesenchymal stem cells and a preparation method thereof. Background technique [0002] Articular cartilage is a special tissue without blood vessels, lymph and innervation. The self-repair ability of articular cartilage itself is very limited, and once the damaged diameter is greater than 3mm, it will not be able to repair itself. At present, the main methods for the treatment of cartilage defects are: arthroscopic lavage, joint debridement, subchondral bone drilling, microfracture, and auto or allo osteochondral transplantation. transplantation), autologous chondrocyte implantation (ACI) and artificial joint replacement. Each of the above methods has surgical indications, advantages and disadvantages, and is not sati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36C12N5/0775
Inventor 金成哲徐燕唐成王黎明
Owner 金成哲
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