Medium for simple, convenient and quick pythium aphanidermatum isolation
A culture medium and technology of Pythium melons and fruits, applied in the field of microorganisms, can solve the problems of difficult separation, low separation rate of target bacteria, failure to achieve separation and purification, etc., and achieve the effect of simple preparation, fast speed, practicality, quickness and cost
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Embodiment 1
[0032] Embodiment 1, three kinds of fungal inhibitors, a kind of Phytophthora inhibitor and three kinds of antibiotics are to Pythium melon and fruit inhibition
[0033] 1. Inhibitory effect of three fungal inhibitors and one Phytophthora inhibitor on Pythium melon and fruit
[0034] Using dimethylformamide as the solvent of carbendazim, using acetone or methanol as the solvent of prochloraz, pentachloronitrobenzene and flumorph, prepare 10ml of four kinds of medicament mother solutions of 5000 μg / mL respectively; The mother liquors of the three fungal inhibitors, bacterazim, pentachloronitrobenzene and prochloraz, respectively prepared a series of drug-containing PDA plates with a concentration of 50 μg / mL, 20 μg / mL and 10 μg / mL; The mother solution of the inhibitor was prepared to prepare a series of drug-containing PDA plates with a concentration of 20 μg / mL, 10 μg / mL and 5 μg / mL, and the plate with solvent was used as a control; , pentachloronitrobenzene, prochloraz and f...
Embodiment 2
[0043] Embodiment 2, three kinds of antibiotics are to the inhibitory effect of bacteria in the environment
[0044] Plants to be tested: Cultivate the susceptible cucumber variety Changchun Mici in a seedling pot with a diameter of 15 cm and a height of 12 cm, and place it in the pepper test field of the Science Park of China Agricultural University. Liquid preparation.
[0045] Soil for testing: Soil samples were collected from areas with severe damping-off disease in Daxing District, Beijing in 2011, for future use.
[0046] Test method: This test is based on the results of Example 1 to detect whether the selected three concentrations of antibiotics can effectively inhibit the bacteria in the environment. For the detection of bacteria in the environment, it is mainly aimed at the bacteria from the base of the plant stem and the diseased soil. This is based on the fact that the isolated parts of Pythium melon and fruit are mainly determined by the base of the diseased stem ...
Embodiment 3
[0049] Embodiment 3, be used for separating the PDA selective culture medium of Pythium melon and fruit
[0050] 1. Separation method
[0051] During 2011 and 2012, disease samples were collected from areas with severe damping-off disease in Daxing, Pinggu, Changping, and Shunyi districts of Beijing for the isolation of Pythium melons and fruits.
[0052] The isolation of Pythium melon and fruit is carried out as follows: select different fields in the same area, and collect damping-off disease samples (rhizome and soil) for isolation.
[0053] Separation step: clean the diseased tissue with clean water and dry it fully, cut out a small piece of tissue at the junction of the rhizome with disease and health, inoculate it on the selective medium of the present invention, and culture it in the dark at 25°C until mycelium grows; Pick the vigorously growing small piece of mycelium into clean water, and culture it at 20°C for 24-48 hours. It can be observed that a large number of s...
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