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Application of stilbeneglycoside to prophylaxis and treatment of osteoporosis

A stilbene glycoside and osteoporosis technology, applied in the field of prevention and treatment of osteoporosis, can solve problems such as damage to cell structure and function, and achieve the effect of improving oxidative stress and inhibiting expression

Inactive Publication Date: 2012-11-21
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

However, high concentrations of ROS can lead to oxidation reactions of nucleic acids, proteins, and lipids, damage the structure and function of cells, and cause diseases

Method used

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  • Application of stilbeneglycoside to prophylaxis and treatment of osteoporosis
  • Application of stilbeneglycoside to prophylaxis and treatment of osteoporosis
  • Application of stilbeneglycoside to prophylaxis and treatment of osteoporosis

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Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0024] MC3T3-E1 is an osteoblast precursor cell line that can differentiate into osteoblasts, and is a more commonly used cell model for studying the growth and differentiation of osteoblasts. H 2 o 2 Treatment is also a commonly used in vitro oxidative stress model. In order to observe the effect of stilbene glycoside on apoptosis and differentiation of osteoblasts, and its anti-oxidative damage, MC3T3-E1 was selected as the cell model. H 2 o 2 Treated as an in vitro model of oxidative stress.

[0025] 1. Establishment of oxidative stress model in vitro

[0026] MC3T3-E1 cells were 2 × 10 3 Inoculate on 96-well plate, discard the medium after the cells are full, add 100, 200, 300, 400 μM / L H 2 o 2 Serum-free culture medium was added to the well plate for 2h, 6h, 12h, and 24h respective...

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Abstract

The invention discloses application of stilbeneglycoside (TSG) to prophylaxis and treatment of osteoporosis. TSG has no toxic effect on osteoblast precursor cells, and does not affect cell proliferation; TSG has protective function on apoptosis of osteoblast precursor cells (MC3T3-E1) caused by hydrogen peroxide (H2O2), improves oxidative stress state induced by H2O2 in a differentiation process of the MC3T3-E1, and has protective function on MC3T3-E1 osteogenic differentiation inhibition caused by H2O2; besides, TSG can inhibit expression of a bone absorption factor. Therefore, TSG can play a role of protecting osteoporosis through improvement on osteoblast apoptosis and differentiation inhibition, and indirect inhibition of osteoclast activation.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of osteoporosis, and relates to the application of stilbene glycosides in the prevention and treatment of osteoporosis. Background technique [0002] Osteoporosis (Osteoporosis, OP) is a degenerative, systemic, and metabolic bone disease. It is caused by a variety of etiologies that result in loss of bone mass, structural changes, and decreased biomechanical properties of bone tissue. It is prone to fractures and poses a serious threat to the elderly. of good health. [0003] Oxidative stress is a state in which highly active substances such as ROS (Reactive oxygen species) are produced excessively in the body, which exceeds the body's ability to remove them, resulting in an imbalance between oxidation and anti-oxidation. ROS in the physiological range play an important role in regulating normal functions of the human body, such as cell apoptosis, gene expression, and signal tran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7034A61K36/704A61P19/10
Inventor 张金康刘建杨柳孟国林袁志罗卓荆
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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