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Vitamin B12 chemiluminescent immunoquantitative detection kit and preparation method thereof

A chemiluminescent immunoassay and quantitative detection technology, which is applied in the field of immunoassay medicine, can solve the problems of narrow detection range, low sensitivity, and high cost, and achieve the effect of simple operation, high sensitivity, and low cost

Active Publication Date: 2015-09-23
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The problem to be solved by the present invention is to provide a chemiluminescent immunoquantitative detection kit for vitamin B12 (VB12) and its preparation method, which solves the defects of low sensitivity, narrow detection range and high cost

Method used

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  • Vitamin B12 chemiluminescent immunoquantitative detection kit and preparation method thereof
  • Vitamin B12 chemiluminescent immunoquantitative detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation of VB12 Chemiluminescent Immunoquantitative Detection Kit

[0038] VB12 chemiluminescent immunoquantitative detection kit, characterized in that the kit includes: VB12 antibody-coated plate, VB12 antigen-enzyme conjugate, VB12 calibrator, VB12 quality control product, 20-fold concentrated lotion, luminescent liquid A liquid and B liquid, sample processing liquid A liquid and B liquid.

[0039] Prepare the VB12 chemiluminescent immunoquantitative detection kit by the following method:

[0040] 1) Preparation of VB12 antibody-coated plates

[0041] Dilute the VB12 antibody with 0.05mol / L pH9.6 carbonate buffer to 1~10ug / mL, add it to a white microwell plate, and coat it at 37°C for 2 hours; discard the liquid in the well, and use pH7.4PBS buffer Wash the plate with liquid, then add 0.5% BSA in phosphate buffer to seal the microwell plate, and seal at 37°C for 2 hours; discard the liquid in the well, spin dry and dry at 37°C for 4 hours; put it int...

Embodiment 2

[0066] Example 2: Preparation of VB12 Chemiluminescent Immunoquantitative Detection Kit

[0067] The kit of the present invention was prepared in the same manner as in Example 1, except that the solid phase carrier was a 48-well white microwell plate.

Embodiment 3

[0068] Embodiment 3: the using method of kit of the present invention

[0069] 1) Equilibrate the test kit at room temperature (18-25°C) for 30 minutes.

[0070] 2) Prepare lotion: Dilute the concentrated lotion with distilled water at 1:20 (1mL lotion plus 19mL distilled water). If the concentrated lotion has crystals, place the concentrated lotion at room temperature or 37°C until the crystals dissolve before diluting.

[0071] 3) Preparation of luminous liquid: 5 minutes before use, take an appropriate amount of luminous liquid A and B and mix them in equal volumes.

[0072] 4) Preparation of sample treatment solution: Prepare sample treatment solution according to the number of test wells. Each well needs 500 μL of sample treatment solution A and 10 μL of sample treatment solution B. The preparation can be increased in an appropriate amount, and it is generally advisable to prepare 10% more. Take an appropriate container to mix the sample treatment solutions A and B. T...

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PUM

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Abstract

VB12 The invention discloses a quantitative determination kit of chemiluminescence immunoassay for Vitamin B12 (VB 12), comprising a VB 12 antibody coated plate, an enzyme conjugated VB 12, a VB 12 calibration material, a quality control material, 20 times concentrated washing liquor, luminescent liquors A and B, and sample treatment fluids A and B. additionally, the invention also discloses a method for preparing the VB 12 chemiluminescence immune quantitative determination kit. The advantages of the kit over the prior art are simple operation, and safety and no environmental pollution. Moreover, the kit has the advantages of ability for samples of broad concentration range detection, long effective period, good stability, low cost and the like.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine, in particular to a vitamin B12 (VB12) chemiluminescent immunoquantitative detection kit and a preparation method thereof. Background technique [0002] Vitamin B12 (vitamin B12, VB12), also known as cobalamin, is a cobalt-containing vitamin and a general term for all corrinoids with biological activity of cyanocobalamin. VB12 in nature is synthesized by microorganisms. Plants cannot make VB12, and VB12 is the only vitamin that requires the help of an intestinal secretion (endogenous factor) to be absorbed. In addition, vitamin B12 is also the only vitamin that contains essential minerals. It is red because of cobalt, also known as red vitamin, and it is one of the few colored vitamins. VB12 belongs to group B vitamins, the storage amount is very small, about 2~3mg is in the liver. The human body needs very little vitamin B12, as long as the diet is normal, it will not be deficient. It is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/82G01N33/543
Inventor 刘萍宋启超董婷婷李克锦范利花
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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