Blueberry tender stem tissue culturing and rapid propagation process

A technology of tissue culture rapid propagation and tender stems, applied in horticulture, horticultural methods, gardening tools/equipment, etc., can solve the problems of blueberry seedlings that cannot meet the growing number of blueberry seedlings, low survival rate, easy to carry viruses, etc., and achieve easy removal Viruses, seedlings with good quality and fast reproduction

Inactive Publication Date: 2012-12-12
SICHUAN LIYI ECOLOGY AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of conventional hard and green branch cuttings has low survival rate, long period, slow speed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Blueberry tender stem segment tissue culture rapid propagation technique, it comprises the following steps:

[0014] S1. Select non-lignified tender stems from the field, wash them with clean water for 50 minutes, wash them with detergent-added water on a sterile workbench for 20 minutes, then disinfect them with 75% alcohol for 30 seconds, rinse them with sterile water for 5 times, and then Put it in 0.1% mercuric chloride disinfectant solution for 8 minutes on the ultra-clean workbench, rinse it with sterile water for 3 times; then cut the tender stems into sections with buds on the ultra-clean workbench, and remove some branches and leaves;

[0015] S2. Induction culture: On the ultra-clean workbench, inoculate the cut stems with buds on the induction medium for budding culture. The induction medium refers to MS+ZT 3.0mg / L+IBA 0.1mg / L+sucrose 30g / L+ agar powder 6.5g / L, adjust its pH to 5.4;

[0016] S3, subculture: after the new shoots with a length of 1 cm grow on ...

Embodiment 2

[0018] Blueberry tender stem segment tissue culture rapid propagation technique, it comprises the following steps:

[0019] S1. Select non-lignified tender stems from the field, wash them with clean water for 60 minutes, wash them with detergent-added water on a sterile workbench for 30 minutes, then disinfect them with 75% alcohol for 20 seconds, and rinse them with sterile water for 3 times. Put it in 0.1% mercuric chloride disinfectant solution for 10 minutes on the ultra-clean workbench, rinse it twice with sterile water; then cut the tender stems into sections with buds on the ultra-clean workbench, and remove some branches and leaves;

[0020] S2. Induction culture: On the ultra-clean workbench, inoculate the cut stems with buds on the induction medium for budding culture. The induction medium refers to MS+ZT 3.0mg / L+IBA 0.1mg / L+sucrose 30g / L+ agar powder 6.5g / L, adjust its pH to 5.2;

[0021] S3, subculture: after the new shoots with a length of 1 cm grow on the tender...

Embodiment 3

[0023] Blueberry tender stem segment tissue culture rapid propagation technique, it comprises the following steps:

[0024] S1. Select non-lignified tender stems from the field, wash them with clean water for 55 minutes, wash them with detergent-added water on a sterile workbench for 25 minutes, then disinfect them with 75% alcohol for 25 seconds, rinse them with sterile water 4 times, and then Put it in 0.1% mercuric chloride disinfectant solution for 9 minutes on the ultra-clean workbench, rinse it with sterile water for 3 times; then cut the tender stems into sections with buds on the ultra-clean workbench, and remove some branches and leaves;

[0025] S2. Induction culture: On the ultra-clean workbench, inoculate the cut stems with buds on the induction medium for budding culture. The induction medium refers to MS+ZT 3.0mg / L+IBA 0.1mg / L+sucrose 30g / L+ agar powder 6.5g / L, adjust its pH to 5.8;

[0026] S3, subculture: after the new shoots with a length of 1 cm grow on the ...

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PUM

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Abstract

The invention discloses a blueberry tender stem tissue culturing and rapid propagation process. The process comprises the following steps: S1, selecting tender stem without lignification from a field, purifying, disinfecting and cutting into stem sections with bud; S2, carrying out induction culturing, wherein the induction culture medium contains MS, 3.0mg/L of ZT (zeatin), 0.1mg/L of IBA (indolebutyric acid), 30g/L of sucrose and 6.5g/L of agar powder; and S3, carrying out subculturing, wherein the multiplication culture medium contains MS, 0.5-1.5mg/L of ZT, 0.05-0.1mg/L of IBA, 30g/L of sucrose and 6.5g/L of agar powder, and carrying out nursery stock rejuvenation by using the culture medium containing MS and0.3-0.7mg/L of ZT before transplanting of the blueberry. The process has the beneficial effects of high reproductive rate, high reproductive speed and low cost of tissue culture seedling; and the cultured nursery stock is high in quality and uniform, can remove virus easily, has developed root systems, and provides a favorable basis for the later-stage tree body growth result.

Description

technical field [0001] The invention relates to the technical field of blueberry tissue culture, in particular to a technique for tissue culture and rapid propagation of blueberry tender stem segments. Background technique [0002] Blueberry belongs to the shrub of Vaccinium genus in the magpie family. It is a small berry fruit tree, and its fruit has high nutritional value and economic value. At present, the demand for blueberries in the market is constantly increasing, but the planting industry of blueberries in my country has just started, and various places are in urgent need of a large number of seedlings of good varieties when establishing plantations. The conventional cuttings of hard branches and green branches have low survival rate, long period, slow speed, and are prone to viruses, which cannot meet the growing needs of the market for the number of blueberry seedlings. Contents of the invention [0003] The purpose of the present invention is to overcome the sh...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈川菊
Owner SICHUAN LIYI ECOLOGY AGRI DEV
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