Bioluminescent Microscopic Detection Instrument
A microscopic detection and fluorescence technology, which is applied in the field of biological fluorescence microscopic detection instruments, can solve the problems of low lateral resolution, complex control system composition, poor axial resolution, etc., and achieve high resolution and high imaging signal-to-noise ratio , Improve the effect of horizontal resolution
Active Publication Date: 2015-08-26
SUZHOU INST OF BIOMEDICAL ENG & TECH
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Problems solved by technology
[0002] Total internal reflection microscopy and confocal microscopy have their own advantages and disadvantages: total internal reflection microscopy has high axial resolution, but its lateral resolution is low; confocal microscopy has high lateral resolution, but poor axial resolution
Although this method generates a superposed image of the total internal reflection fluorescence image and the confocal image, because the two microscopes work successively, it is physically impossible to obtain a three-dimensional high-resolution image at the same time; in addition, the invention needs to operate two microscopes successively to shoot images, and to overlap the images, the structure of the microscope is more complicated, and the composition of the control system is also more complicated
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Embodiment
[0021] Embodiment: The biological fluorescence microscopic detection instrument 100 includes an excitation light illumination unit 110 , an excitation light and fluorescence isolation unit 120 , a laser scanning unit 130 , a microscopic imaging unit 140 , a fluorescence detection unit 150 and a control unit 160 .
[0022] The excitation light illumination unit 110 includes a laser light source 111 , a first beam expander 112 , and a second beam expander 113 . An illumination pinhole 114 is provided between the first beam expander 112 and the second beam expander 113 and at the focal point of the first beam expander 112 .
[0023] The excitation light and fluorescence isolation unit 120 includes an excitation light color filter 121 , a dichromatic mirror 122 , and a fluorescence color filter 123 . The 121 excitation light color filters are used to receive laser light, filter out light beams deviated from the center wavelength in the laser light, and transmit light beams at the ...
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The invention provides a biological fluorescence microscopic detection instrument which comprises an excitation light illumination unit, excitation light, a fluorescence isolation unit, a laser scanning unit, a microimaging unit, a fluorescence detection unit and a control unit. An illumination pinhole is arranged between a first beam expander and a second beam expander of the excitation light illumination unit and located at a focal point of the first beam expander, an imaging detection pinhole is arranged between an imaging lens and a photomultiplier of the fluorescence detection unit and located at a focal point of the imaging lens, and therefore, the transverse resolution of the instrument is effectively improved; meanwhile, an annular beam shaping assembly is arranged at an entrance pupil position of a microobjective of the microimaging unit, which enables the axial resolution of the biological fluorescence microscopic detection instrument to be improved.
Description
technical field [0001] The invention relates to the field of design and manufacture of microscopic detection instruments, in particular to a biological fluorescence microscopic detection instrument. Background technique [0002] Total internal reflection microscopy and confocal microscopy have their own advantages and disadvantages: total internal reflection microscopy has high axial resolution, but its lateral resolution is low; confocal microscopy has high lateral resolution, but poor axial resolution. How to utilize both the high axial resolution of total internal reflection microscopy and the high lateral resolution of confocal microscopy in one microscope is particularly important. [0003] In the invention patent application CN201080024155.9, an image processing device, program and microscope for simply and correctly superimposing total internal reflection fluorescence images and confocal images are proposed. This invention includes two sets of microscopes, one is a t...
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IPC IPC(8): G02B21/06G02B21/36G01N21/64
Inventor 张运海张欣黄维昌剑薛晓君
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH