Universal cell rapid breaker using bead vibrating and grinding

Abstract

A universal cell rapid breaker using bead vibrating and grinding mainly comprises a main shell, a driving module, a control module, a power shaft, a rotor, a transmission mechanism, centrifuge tube supports, centrifuge tubes and grinding beads. The driving module fixed on the main shell provides power source for the whole breaker under control of the control module, and transmits motion and torque to the rotor through the power shaft so as to force the rotor to rotate with a high speed. Initiating terminal of the transmission mechanism is connected with the rotating rotor through a higher pair, and transforms the high-speed rotary movement of the rotor to a high-frequency reciprocating linear movement of the terminal of the transmission mechanism. The centrifuge tube supports are fixed on at the terminal of the transmission mechanism and do the high-frequency reciprocating linear movement along with the terminal of the transmission mechanism. The centrifuge tubes with the grinding beads and cell suspension are put in and fixed on the centrifuge tube supports, and do the high-frequency reciprocating linear movement along with the centrifuge tube supports, so that the grinding beads vibrate in a high frequency, and mutually violently shear and impact with cells, thereby resulting in cells disruption to release intracellular active substances.

Description

technical field [0001] The invention belongs to the technical field of biological sample preparation, and is suitable for cell crushing in genetic engineering and cell engineering, specifically using high-frequency fluttering grinding beads to quickly crush cells to obtain intracellular active substances, such as DNA, RNA, enzymes, antibiotics, etc. . Background technique [0002] In the field of genetic engineering and cell engineering technology, it is often necessary to obtain active substances such as DNA, RNA, and enzymes in cells, that is, sample preparation. The first step in sample preparation is to break up the peripheral structures of the cells (cell walls and cell membranes), so that the active substances to be extracted are released from the cells. It can be seen that the degree and efficiency of cell disruption directly affect the quality of sample preparation, and even determine the success or failure of sample preparation, and its importance is self-evident. ...

AI Technical Summary

Problems solved by technology

[0012] To sum up, although there are many cell disruption methods, most of them can only break some easily broken cells such as animal cells and Gram-negative bacteria, and usually cannot break Gram-positive bacteria and yeasts. , bacterial spores, etc., the scope of application is quite limited

Although the bead mill crushing method can adapt to various types of cell crushing, there are still many defects and deficiencies in the corresponding equipment, and the crushing effect is not ideal.

Images