Method for inducing accumulation of stilbenes in grapes

A technology of grapes and substances, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve problems affecting the health of eaters, environmental toxicity, and operator poisoning, so as to avoid photodecomposition, avoid temperature rise, Simple operation effect

Inactive Publication Date: 2012-12-26
张波 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] When analyzing the above-mentioned technical scheme of chemically inducing grape resveratrol accumulation, it was found that there were the following deficiencies: i. The effect is not good; ii. The above-mentioned induction scheme has a low specificity on the synthetic pathway of stilbenes, which may affect the quality and yield of grapes during the in vivo induction process; iii. The induction characteristics of glycosides and pterostilbene are unknown; iv. The above induction scheme does not take into account the difficulty of actual operation. In field operations, the selection of materials and the use of inducers determine the induction cost. Induction techniques such as stem injection, phosphonium aluminum in conjunction with UV light treatment, and copper sulfate in conjunction with chitosan are time-consuming and laborious to implement, and large-scale...

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  • Method for inducing accumulation of stilbenes in grapes
  • Method for inducing accumulation of stilbenes in grapes
  • Method for inducing accumulation of stilbenes in grapes

Examples

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Embodiment 1

[0049] Take commercially available hydrogen peroxide concentration of 50% hydrogen peroxide, add water to dilute to a final hydrogen peroxide concentration of 2.5%, add surfactant Triton X-100 so that its weight percentage final concentration is 0.01 as a liquid inducer for subsequent use; prepare the above steps The obtained liquid inducer sprays the American red grape vines in the young shoot growth period in the field, and sprays 10 parts by weight of the liquid inducer per 100 parts by weight of the grape vine to induce treatment for 8 hours; the total stilbene in the fruit is measured by HPLC. The average fresh weight content of stilbene substances increased to 1.5 times, the average fresh weight content of stilbene substances in leaves increased to 3.3 times, and the average fresh weight content of stilbene substances in stems increased to 1.1 times. The average fresh weight content of stilbene substances in leaves was 12 μg / g for resveratrol, 42 μg / g for resveratrol glyc...

Embodiment 2

[0051] Get commercially available hydrogen peroxide concentration of 28% hydrogen peroxide, add water to dilute to hydrogen peroxide final concentration of 5%, add surfactant Triton X-100 so that its weight percentage final concentration is 0.05 as liquid inducer for subsequent use; The obtained liquid inducer is sprayed to the field planting flowering stage Yatomi rosa vine, and the aboveground part of the grape vine is induced by spraying 5 parts by weight of the liquid inducer per 100 parts by weight for 16 hours; HPLC measures the total stilbenes in the fruit The fresh weight content of substances increased to 3.1 times on average, the fresh weight content of total stilbene substances in leaves increased to 4 times on average, and the fresh weight content of stilbene substances in stems increased to 2 times on average. The average fresh weight content of stilbene substances in leaves was 15 μg / g for resveratrol, 44 μg / g for resveratrol glycosides, and 3.3 μg / g for pterostil...

Embodiment 3

[0053] Take commercially available hydrogen peroxide with a hydrogen peroxide concentration of 20%, add water to dilute to a final hydrogen peroxide concentration of 10%, add surfactant Triton X-100 so that its weight percent final concentration is 0.1% as a liquid inducer for subsequent use; the above steps The prepared liquid inducer is sprayed on the seedless purple grape vines in the growth period of the berry planted in the field, and the aerial part of the grape vine is induced by spraying 1 part by weight of the liquid inducer per 100 parts by weight for 24 hours; the total stilbene in the fruit is measured by HPLC The average fresh weight content of stilbene substances in leaves increased to 6 times, the average fresh weight content of stilbene substances in leaves increased to 16 times, and the average fresh weight content of stilbene substances in stems increased to 5 times. The average fresh weight content of stilbene substances in leaves was 110 μg / g for resveratrol...

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Abstract

The invention relates to a method for inducing the accumulation of stilbenes resveratrol, polydatin and pterostilbene in grape leaves, grape stems and grape fruits. The method comprises related methods for preparation of a hydrogen peroxide inducer, spraying treatment of in-vivo and in-vitro materials of grapes and impregnation treatment of the in-vitro materials of the grapes. According to the technical scheme, increase of stilbene content of the materials can be effectively, specifically and comprehensively induced, and the method has the characteristics of safety and convenience for large-scale application to township enterprises with rich grape resources, and is easy to operate. The quality of the grapes can be improved, grape planting waste can be comprehensively utilized, and grape trees can resist pathogenic bacteria to a certain extent to reduce loss caused by diseases.

Description

technical field [0001] The invention relates to a method for inducing the accumulation of stilbene substances resveratrol, resveratrol glycosides and pterostilbene in grape leaves, grape stems and grape fruits. Background technique [0002] Stilbenes are important constituents of phytoalexins, which exist in some medicinal plants and edible plants, such as cassia, hellebore, knotweed, grapes, and peanuts. Its biosynthesis depends on the secondary metabolic pathway of plant phenylpropane, and the produced stilbene substances, chalcones, lignin and other products are used as phytoalexins, UV protection factors, flower and fruit pigments, cell wall structural components and signal transduction molecules, etc. , play an important role in the physiological and pathological processes of plant stress [1] . Resveratrol is the most important substance synthesized by stilbene synthase (STS). Other key enzymes in this pathway include phenylalanine ammonia lyase (PAL), cinnamate-4-hy...

Claims

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Application Information

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IPC IPC(8): A01G7/06
Inventor 张波王晓琴郑秋生杨晓燕田春芳刘景磊颜欢
Owner 张波
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