Method for inducing accumulation of stilbenes in grapes

Abstract

The invention relates to a method for inducing the accumulation of stilbenes resveratrol, polydatin and pterostilbene in grape leaves, grape stems and grape fruits. The method comprises related methods for preparation of a hydrogen peroxide inducer, spraying treatment of in-vivo and in-vitro materials of grapes and impregnation treatment of the in-vitro materials of the grapes. According to the technical scheme, increase of stilbene content of the materials can be effectively, specifically and comprehensively induced, and the method has the characteristics of safety and convenience for large-scale application to township enterprises with rich grape resources, and is easy to operate. The quality of the grapes can be improved, grape planting waste can be comprehensively utilized, and grape trees can resist pathogenic bacteria to a certain extent to reduce loss caused by diseases.

Description

technical field [0001] The invention relates to a method for inducing the accumulation of stilbene substances resveratrol, resveratrol glycosides and pterostilbene in grape leaves, grape stems and grape fruits. Background technique [0002] Stilbenes are important constituents of phytoalexins, which exist in some medicinal plants and edible plants, such as cassia, hellebore, knotweed, grapes, and peanuts. Its biosynthesis depends on the secondary metabolic pathway of plant phenylpropane, and the produced stilbene substances, chalcones, lignin and other products are used as phytoalexins, UV protection factors, flower and fruit pigments, cell wall structural components and signal transduction molecules, etc. , play an important role in the physiological and pathological processes of plant stress [1] . Resveratrol is the most important substance synthesized by stilbene synthase (STS). Other key enzymes in this pathway include phenylalanine ammonia lyase (PAL), cinnamate-4-hy...

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Problems solved by technology

[0008] When analyzing the above-mentioned technical scheme of chemically inducing grape resveratrol accumulation, it was found that there were the following deficiencies: i. The effect is not good; ii. The above-mentioned induction scheme has a low specificity on the synthetic pathway of stilbenes, which may affect the quality and yield of grapes during the in vivo induction process; iii. The induction characteristics of glycosides and pterostilbene are unknown; iv. The above induction scheme does not take into account the difficulty of actual operation. In field operations, the selection of materials and the use of inducers determine the induction cost. Induction techniques such as stem injection, phosphonium aluminum in conjunction with UV light treatment, and copper sulfate in conjunction with chitosan are time-consuming and laborious to implement, and large-scale induction cannot be achieved; v. The above induction scheme does not take into account the residual toxicity of the inducer in soil and plants, The phytotoxicity of acidic aluminum and soluble copper salts in agricultural production has been recognized, and hormone reagents also have certain residual toxicity. These inducing reagents will accumulate in the soil during use, causing certain environmental toxicity, affecting subsequent crop planting, and also It will remain in plants and fruits, affecting the health of eaters; vi. The above induction scheme does not take into account the impact on operators when it is applied in the field. Acidic aluminum, phosphonium aluminum, copper sulfate, and plant hormones all have certain animal toxicity , the actual operation may cause certain poison to the operator, and even produce retention in the fruit, which is potentially toxic

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