Method for testing trace carbofuran

A detection method, a micro-trace technology, applied in the preparation of test samples, color/spectral characteristic measurement, etc., can solve the problems of large reagent consumption, limit the wide application of chromatography, and high cost of chromatography, and achieve cheap price and source Wide range of effects with high detection efficiency

Active Publication Date: 2012-12-26
LUZHOU PINCHUANG TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because this type of detection method requires large-scale equipment, professional operators and consumes a large amount of reagents, the cost of chromatography detection is very high, thus limiting the wide application of chromatography.

Method used

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  • Method for testing trace carbofuran
  • Method for testing trace carbofuran
  • Method for testing trace carbofuran

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: combined with figure 1 , a method for detecting micro-trace amounts of carbofuran, the sample to be tested in this embodiment is sugarcane, and the specific detection steps are as follows:

[0048] Step 1: Using commercially available flour as raw material, mix flour and distilled water at a weight ratio of 1:5 to obtain a batter, stir the batter to mix the flour and water evenly, and then prepare the batter at a centrifugal speed of 4000r / min Centrifuge for 10 minutes, collect the supernatant, and obtain the crude enzyme;

[0049] Step 2: Take 1 mL of the crude enzyme H in step 1 and add the crude enzyme to 27% PEG1000 / 13% NaH 2 PO 4 In the system, stir and mix evenly, after static phase separation, take out the PEG phase, and add the taken out PEG phase to 27%PEG1000 / 13%NaH 2 PO 4 / 6%(NH 4 ) 2 SO 4 In the system, stir and mix evenly, after static phase separation, dialyze, concentrate and freeze-dry the inorganic salt phase composed of sodium...

Embodiment 2

[0059] Example 2: combined with figure 2 , step 1 and step 2 in this embodiment are the same as step 1 and step 2 in embodiment 1, and steps 3-8 are specifically as follows:

[0060] Step 3: the concentration of tetraphenyl zinc porphyrin solution is 70 μmol / L;

[0061] Step 4: Take 0.1 mL of plant esterase enzyme solution, 0.05 mL of tetraphenyl zinc porphyrin solution and 4.7 mL of phosphate buffer solution with a pH value of 6.9 and mix evenly to obtain a tetraphenyl zinc porphyrin-plant esterase compound solution;

[0062] Step 5: Use an ultraviolet spectrophotometer to detect the absorption spectrum curve of the tetraphenyl zinc porphyrin-plant esterase compound solution prepared in step 4 for light waves with a wavelength of 300nm-750nm, that is, curve (1);

[0063] Step 6: Take 0.1 mL of plant esterase enzyme solution, 0.05 mL of tetraphenyl zinc porphyrin solution and 4.7 mL of phosphate buffer solution with a pH value of 6.9 and mix evenly, then add 0.5 mL of suga...

Embodiment 3

[0066] Example 3: combined with image 3 , step 1 and step 2 in this embodiment are the same as step 1 and step 2 in embodiment 1, and steps 3-8 are specifically as follows:

[0067] Step 3: the concentration of tetraphenyl zinc porphyrin solution is 90 μmol / L;

[0068] Step 4: Take 0.1 mL of plant esterase enzyme solution, 0.1 mL of tetraphenyl zinc porphyrin solution and 4.81 mL of phosphate buffer solution with a pH value of 7.0 and mix evenly to obtain a tetraphenyl zinc porphyrin-plant esterase compound solution;

[0069] Step 5: Use an ultraviolet spectrophotometer to detect the absorption spectrum curve of the tetraphenyl zinc porphyrin-plant esterase compound solution prepared in step 4 for light waves with a wavelength of 300nm-750nm, that is, curve (1);

[0070] Step 6: Take 0.1 mL of plant esterase enzyme solution, 0.1 mL of tetraphenyl zinc porphyrin solution and 4.81 mL of phosphate buffer solution with a pH value of 7.0 and mix evenly, then add 0.5 mL of sugar...

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Abstract

The invention relates to the technical field of chemical pesticide test, and particularly relates to a method for testing carbofuran in carbamic acid ester pesticides, in particular to a method for testing trace carbofuran. With flour, zinc meso-tetraphenylporphine solution and phosphate buffer with the pH value of 6.5 to 7.25 as materials, the method respectively prepares zinc meso-tetraphenylporphine-plant esterase compound solution and sample mixed solution, then respectively tests the absorption spectrum curves of the zinc meso-tetraphenylporphine-plant esterase compound solution and the sample mixed solution on light waves with the wavelength of 300nm to 750nm, and compares the two tested spectrum curves; if the two spectrum curves are identical, then N, N-dimethylformamide cleaner sample to be tested does not contain carbofuran, or the content of carbofuran exceeds the test limit of the method; and if the two spectrum curves are not identical, then the sample to be tested contains carbofuran. The test cost of the method is low, the method is applicable to a wide range of regions, the precision, repeatability and stability of the test result are high, field tests can be carried out as well, the method is easy to operate, and moreover, the affection of the environment on the test result is little.

Description

technical field [0001] The invention relates to the technical field of chemical pesticide detection, in particular to a method for detecting carbofuran in carbamate pesticides, and in particular to a method for detecting micro-trace amounts of carbofuran. [0002] Background technique [0003] Carbofuran belongs to carbamate pesticides, and carbamate pesticides are one of the three commonly used pesticides. This type of pesticide is often used to treat the seeds of crops, and can effectively suppress a variety of pests. [0004] Carbofuran is a highly toxic insecticide with no irritation to eyes and skin. In the test dose, it has no teratogenic, mutagenic, or carcinogenic effects on animals, is highly toxic to fish and birds, and is non-toxic to bees. However, due to the high toxicity and refractory properties of carbofuran, its appropriate dosage and correct use directly affect its effect on humans and the environment. On vegetables, fruits and other directly edible crop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N1/34
Inventor 侯长军沈才洪何昆张良霍丹群张宿义彭云江勇
Owner LUZHOU PINCHUANG TECH CO LTD
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