Improved method for converting plant pollen tube

A plant pollen and plant technology, applied in the field of improved plant pollen tube transformation, can solve the problems of low transformation efficiency, easy degradation of exogenous plasmid DNA and the like
CN102864168BInactive Publication Date: 2014-04-30JILIN AGRICULTURAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JILIN AGRICULTURAL UNIV
Publication Date
2014-04-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an improved method for converting a plant pollen tube. Aluminum hydroxide sol, calcium phosphate and Tween20 are adopted respectively to serve as exogenous plasmid DNA protective agents, and a plant pollen tube channel method is utilized to convert an exogenous gene. Receptor tobacco and soybean GUS active tissue histochemical staining results show that the gus positive rates with the aluminum hydroxide sol serving as the DNA protective agent are respectively 11.1% and 3.78%; the gus positive rates with calcium phosphate serving as the DNA protective agent are respectively 8.89% and 2.91%; the gus positive rates with Tween20 serving as the DNA protective agent are respectively 10.0% and 1.41 %; and the gus positive rates with 1*SSC solution serving as the DNA protective agent are respectively 7.78% and 1.97 %, the problem that the conversion rate in the plant pollen tube channel method is low is solved, and the conversion rate is improved.
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Description

technical field

[0001] The invention relates to the field of biotechnology genetic breeding, in particular to an improved plant pollen tube transformation method. Background technique

[0002] In 1981, Chinese scientist Zhou Guangyu introduced exogenous DNA into upland cotton for the first time by using the pollen tube passage method, and successfully bred a transgenic variety resistant to Fusarium wilt. In recent years, due to its unique advantages, the pollen tube passage method has been widely used by scientists for transgenic operations of plants. This method has the following advantages: First, the pollen tube passage method has a wide range of applications and can be applied to any flowering plants, and different gene transfers can be performed between different species, so it relaxes the target gene and receptor range of plants. Second, it does not rely on the cumbersome plant tissue culture and the long process of inducing plant regeneration. The pollen tube passag...

Claims

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