33 results about "DNA protection" patented technology

The invention relates to a method for purifying deoxyribonucleic acid (DNA) in a DNA bisulfate conversion process and belongs to the technical field of nucleic acid conversion and purification. According to the method disclosed by the invention, a DNA protecting agent capable of preventing DNA from fragmenting and degrading is added to a DNA bisulfate conversion system, so that the DNA is not fragmented or degraded in the treatment process of bisulfate. Thus, the quality of the processed DNA is effectively improved; the yield of nucleic acid is about 70%; and the sensitivity of polymerase chain reaction (PCR) and other analysis technologies is improved. By adopting the method disclosed by the invention, the entire treatment process of the DNA bisulfate is simpler and faster; and meanwhile, the quality of the processed DNA is improved.

The invention discloses an improved method for converting a plant pollen tube. Aluminum hydroxide sol, calcium phosphate and Tween20 are adopted respectively to serve as exogenous plasmid DNA protective agents, and a plant pollen tube channel method is utilized to convert an exogenous gene. Receptor tobacco and soybean GUS active tissue histochemical staining results show that the gus positive rates with the aluminum hydroxide sol serving as the DNA protective agent are respectively 11.1% and 3.78%; the gus positive rates with calcium phosphate serving as the DNA protective agent are respectively 8.89% and 2.91%; the gus positive rates with Tween20 serving as the DNA protective agent are respectively 10.0% and 1.41 %; and the gus positive rates with 1*SSC solution serving as the DNA protective agent are respectively 7.78% and 1.97 %, the problem that the conversion rate in the plant pollen tube channel method is low is solved, and the conversion rate is improved.

The preparation of extracts from olive fruits, olive tree leaves, olive oil as well as olive-press waste. The isolation of natural products from these extracts and the evaluation of the DNA protective antioxidant activity of the extracts and the purified compounds on intact cells.

The invention discloses a genome DNA (Deoxyribonucleic Acid) preservation solution of a saliva and oral cavity swab. The genome DNA preservation solution is characterized in that the preservation solution comprises the following components according to the concentration of 1 liter of the preservation liquor: 0.10 to 0.2 percent of SDS (sodium dodecyl sulfate), 0.90 percent of NaCl, 5 to 15mM of Tris(trihydroxymethylaminomethane), 20 to 25mM of EDTA (Ethylene Diamine Tetraacetic Acid) and 150 to 160mg of proteinase K. The preservation solution of the saliva and oral cavity swab takes the SDS,Tris-HCl, the EDTA, the NaCl and the proteinase K as main components and can be used for preserving saliva. The NaCl can be used for maintaining osmotic pressure of cells; an SDS surfactant can be used for damaging a protein component and inhibiting the breeding of bacteria under the action of the proteinase K and the degradation of DNA is easy to cause; and the Tris-HCl and the EDTA can be used for protecting free DNA. The DNA preservation solution provided by the invention is a preservation solution aiming at the preservation of the oral cavity swab and can be used for preserving oral cavitycells relatively well; the DNA preservation solution is more sanitary and the bacteria are not easy to breed; the preservation time is 3 to 5 times longer than the preservation time in an envelope; and a preparation method is simple and has relatively good social value.

The present invention relates to a functional health-care food with the functions of resisting radiation, resisting free radical and protecting DNA. Its preparation method includes the following steps: making ganoderma spore powder undergo the process of low-temp. antioxidative wall-breaking treatment; water-extracting or alcohol-extracting ganoderma sporocarp to obtain its coarse polysaccharide; using any one method described in the application specification to extract jinsiyi (a Chinese medicinal material) to obtain its polysaccharide; extracting tea to obtain tea-polyphenol; mixing the above-mentioned materials and making them into capsule or tablet preparation so as to obtain the invented product.

The invention relates to a method for purifying deoxyribonucleic acid (DNA) in a DNA bisulfate conversion process and belongs to the technical field of nucleic acid conversion and purification. According to the method disclosed by the invention, a DNA protecting agent capable of preventing DNA from fragmenting and degrading is added to a DNA bisulfate conversion system, so that the DNA is not fragmented or degraded in the treatment process of bisulfate. Thus, the quality of the processed DNA is effectively improved; the yield of nucleic acid is about 70%; and the sensitivity of polymerase chain reaction (PCR) and other analysis technologies is improved. By adopting the method disclosed by the invention, the entire treatment process of the DNA bisulfate is simpler and faster; and meanwhile, the quality of the processed DNA is improved.

The invention provides a quality control product of a chromosome aneuploidy detection kit capable of being stably stored, and application. The quality control product comprises a positive reference product and a negative reference product, wherein the positive reference product contianins DNA protecting agents and DNA fragments from 150 to 200bp intervals with the nuclear type analysis result being 47 and TN1 human genome DNA amplification products; the negative reference product contains a DNA protecting agents and DNA fragments which are not from 150 to 200bp intervals with the nuclear typeanalysis result being 47 and TN1 human genome DNA amplification products; the TN1 is selected from one several kinds of materials from T21, T18 and T13; the DNA protection agents contain aurintricarboxylic acid ammonium salt, glycine and bestatin hydrochloride. The quality control product provided by the invention has the advantages that the protection agents are added into each reference product;the DNA degradation of the reference product in the quality control product is inhibited, so that the stability is higher; the storage is easy; the shelf life is prolonged.

A composition having at least one of a high antioxidant capacity, an anti-inflammatory effect and / or a DNA protection function is provided. The composition includes an extract from plants of the genus Caesalpinia, for example, Caesalpinia spinosa, also known as Tara. The Tara extract can be included in a nutritional supplement or other delivery vehicle and administered to a subject. Methods for using the composition also are provided.

The invention discloses a collagen for skin repair and a preparation method thereof. The present invention uses sea cucumbers as raw materials, adopts methods such as ultra-high pressure treatment, protease enzymatic hydrolysis, etc. to process sea cucumbers, and simultaneously uses deodorizing agents to deodorize the enzymatic solution, so that the prepared collagen for skin repair has a high content of active ingredients. It has good solubility, high absorption, and no fishy smell. It has various functions such as anti-oxidation, anti-inflammation, DNA protection, moisturizing ability, anti-wrinkle function and skin whitening effect. It can be used as a raw material for cosmetics, nutritional health products, food and Medical and other fields.

The invention discloses an exopolysaccharide and its application, belonging to the technical field of bioengineering. The exopolysaccharide (EPS) of the present invention is a kind of glucosamine, arabinose, galactosamine, galactose, glucose and Heteropolysaccharide composed of mannose, which has higher ability to scavenge free radicals than ascorbic acid; can provide good DNA protection for oxidative stress induced by AAPH and hydroxyl radicals; at the same time, it can also prevent direct damage to DNA activity by ultraviolet radiation destroy.

The invention provides a quality control product and application of a stable chromosomal aneuploidy detection kit, including a positive reference product and a negative reference product; 1 DNA fragments and DNA protection agents in the 150-200bp interval of the amplification product of human genomic DNA; 1 DNA fragments and DNA protection agents in the 150‑200bp interval of the amplification product of human genomic DNA; the TN 1 One or more selected from T21, T18, T13; the DNA protection agent includes ammonium aurintricarboxylate, glycine, bestatin hydrochloride. The quality control product provided by the present invention inhibits the DNA degradation of the reference product in the quality control product by adding a protective agent to each reference product, making it more stable, easier to store, and prolonging the validity period.

The invention discloses an environment-friendly DNA preservation product, which is to sandwich DNA in a hard urea formaldehyde polymer resin containing a DNA protectant. A concrete production method comprises the steps: extracting the DNA; preparing urea formaldehyde monomers; synthesizing an urea formaldehyde polymer resin liquid containing the DNA protectant; pouring the synthesized urea formaldehyde polymer resin liquid containing the DNA protectant into a mold; compregnating the prepared DNA into the urea formaldehyde polymer resin liquid containing the DNA protectant; and drying the ureaformaldehyde polymer resin liquid containing the DNA protectant to be hardened, and demolding. The product has the advantages of simple production, low cost, energy consumption-free preservation, long preservation period and so on.

The invention discloses a method for rapidly and efficiently extracting genomic DNA of mammal ear tissue. The method comprises steps as follows: 1) tissue disruption and DNA protection; 2) tissue lysate digestion; 3) Tris-saturated phenol extraction; 4) Tris-saturated phenol and chloroform / isoamyl alcohol mixed extraction; 5) chloroform / isoamyl alcohol extraction; 6) precipitation of DNA with NaAc and fully precooled (subzero 20 DEG C) absolute ethanol; 7) ethanol rinsing; 8) dissolution of DNA with a TE buffer solution and preservation. The yield of DNA is significantly increased (the concentration of DNA is 112-851 ng / mu L), meanwhile, the time spent on addition of proteinase K for digestion overnight (longer than or equal to 12 h) after cell disruption during DNA extraction is saved, and about 3 h is spent from tissue disruption to genomic DNA extraction.

The invention discloses an improved method for converting a plant pollen tube. Aluminum hydroxide sol, calcium phosphate and Tween20 are adopted respectively to serve as exogenous plasmid DNA protective agents, and a plant pollen tube channel method is utilized to convert an exogenous gene. Receptor tobacco and soybean GUS active tissue histochemical staining results show that the gus positive rates with the aluminum hydroxide sol serving as the DNA protective agent are respectively 11.1% and 3.78%; the gus positive rates with calcium phosphate serving as the DNA protective agent are respectively 8.89% and 2.91%; the gus positive rates with Tween20 serving as the DNA protective agent are respectively 10.0% and 1.41 %; and the gus positive rates with 1*SSC solution serving as the DNA protective agent are respectively 7.78% and 1.97 %, the problem that the conversion rate in the plant pollen tube channel method is low is solved, and the conversion rate is improved.

The invention discloses a cationic silk fibroin protein and a preparation method thereof, belonging to the technical field of biomedical polymer materials. Cationized silk fibroin by water-soluble 2‑iminothiolane hydrochloride-mediated protamine sulfate coupling via thiosuccinimidyl‑4‑[N‑maleimidomethyl] ‑Cyclohexane‑1‑carboxylate activated silk fibroin, which has good biocompatibility and degradability, controllable surface charge density, effective compression and protection of DNA, high transfection Efficiency, unique cell targeting and nuclear localization capabilities. The cationic silk fibroin protein provided by the present invention can form a cationic silk fibroin / gene complex with genetic material through electrostatic interaction, and is a new type of biodegradable cationic silk fibroin gene transfer with cell targeting and cell nucleus localization functions carrier.