Environment protection type DNA preservation product and manufacturing method thereof

A production method and an environment-friendly technology, applied in the field of DNA preservation, can solve the problems of difficulty in popularizing high-tech technology, consume large amounts of energy, and store for a long time, and achieve the effects of saving power and energy, low cost, and simple production.

Inactive Publication Date: 2012-06-06
DALIAN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main advantage is that the storage time is long, but it needs to consume a lot of energy, and the problem of partial DNA degradation still exists, and if it is to be made into dry powder, a large amount of DNA is required, and it is difficult to obtain materials
[0016] 4. Recently, some scholars have proposed to preserve DNA for more than 100 years by DNA nano-molecule coating technology, but this high-tech technology is difficult to popularize

Method used

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  • Environment protection type DNA preservation product and manufacturing method thereof
  • Environment protection type DNA preservation product and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] Embodiment of the present invention is to carry out according to the following steps:

[0036] a. DNA extraction

[0037] Mainly refer to the conventional phenol / chloroform extraction method of peripheral blood DNA. Specific steps are as follows:

[0038] ① Take out 0.5ml of sterile anticoagulated blood, put it into an Eppendorf tube containing 0.5ml of PBS buffer, mix gently, and centrifuge at 8000rpm for 5-8 minutes, carefully discard the supernatant, and leave about 100μl of liquid precipitate.

[0039] ② Add 180 μl of TE (PH8.0), 20 μl of 10% SDS, 3 μl of proteinase K, and digest overnight at 37°C.

[0040] ③Extract twice with 300μl phenol, and centrifuge at 10000rpm×10min; absorb the upper aqueous phase into another tube, add 150μl phenol and 150μl chloroform, mix well, and centrifuge at 10000rpm×10min.

[0041] ④ Aspirate the upper aqueous phase into another tube, add 1 / 10 volume of NaAC and 2 times the volume of absolute ethanol, and mix slowly to make the whi...

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Abstract

The invention discloses an environment-friendly DNA preservation product, which is to sandwich DNA in a hard urea formaldehyde polymer resin containing a DNA protectant. A concrete production method comprises the steps: extracting the DNA; preparing urea formaldehyde monomers; synthesizing an urea formaldehyde polymer resin liquid containing the DNA protectant; pouring the synthesized urea formaldehyde polymer resin liquid containing the DNA protectant into a mold; compregnating the prepared DNA into the urea formaldehyde polymer resin liquid containing the DNA protectant; and drying the ureaformaldehyde polymer resin liquid containing the DNA protectant to be hardened, and demolding. The product has the advantages of simple production, low cost, energy consumption-free preservation, long preservation period and so on.

Description

Technical field: [0001] The invention relates to a DNA preservation method, in particular to an environment-friendly DNA preservation product and a production method which are simple to manufacture, low in cost, energy-free in preservation and long in preservation period. Background technique: [0002] DNA (Deoxyribonucleic acid, deoxyribonucleic acid) is an important component of chromosomes, as well as the chemical essence of genes, and is recognized as the basic substance of heredity. DNA is the carrier of genetic information, and the order of its four constituent bases, A, G, C, and T, determines the various characteristics of life. DNA, as an important object of molecular genetics research, has been playing an important role in life science research for a long time. For example, many experiments such as genome sequencing technology, comparative genomic hybridization, southern hybridization, SNP genotyping and forensic paternity testing widely used in the Human Genome P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10G01N1/28
Inventor 张开立孔庆友李嘉芝姜美曦宁静赵玉龙朱敬伟孙萍雷钧朱胜男刘佳孙嫒王茜李宏马景昕
Owner DALIAN MEDICAL UNIVERSITY
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