Mouse ascites inducer and application thereof

Abstract

The invention provides a novel mouse ascites inducer. The mouse ascites inducer contains liquid paraffin, wool grease, bacillus calmette guerin vaccine, interleukin and B lymphocyte stimulating factors which are used as active ingredients. The inducer can directly stimulate hybridoma cells to quickly proliferate in the body of a mouse, and secrete a large amount of antibodies. The production cycle of ascites is short, a large amount of ascites is generated, and high titer is guaranteed. When a product is used for preparing a large amount of the ascites antibodies of the mouse, cost is saved; and the inducer has the considerable economic benefit.

Description

technical field [0001] The invention relates to the field of antibody preparation, in particular to a mouse ascites inducer and its application. Background technique [0002] At present, mice of the same strain as hybridoma SP20 are generally used in the preparation of mouse ascites antibodies. Commonly used inducers are pristane and liquid paraffin. These two inducers are all immunosuppressants, which inhibit the normal immune function of animals and promote tumor growth (Hoogenraad, N.J. and Wraight, C.J., The effect of Pristane on Pristane on ascites tumor formation and monoclonal antibody production, Methods Enzymol., 121, 375 ,1986). Using pristane for induction, each mouse can collect about 5-10ml ascites. However, the inducer pristane is expensive and not suitable for large-scale production. In addition, pristane is often used in immune animal disease models, such as arthritis, pristane nephropathy, etc., which shows that pristane itself will cause certain health ...

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Problems solved by technology

In contrast, incomplete adjuvant has a better induction effect than the above two inducers, but this pre-stimulation method will bring more contamination of cytokines and other inflammatory mediators to ascites

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