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Early pregnancy detection test strip and detection method thereof

A technology for detecting test strips and test strips, which is applied in the field of medical testing and can solve problems such as not being able to know the time of pregnancy

Active Publication Date: 2013-01-16
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the early pregnancy test strips on the market use the colloidal gold method for detection, but they can only tell whether the subject is pregnant or not, and cannot know the approximate pregnancy time

Method used

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  • Early pregnancy detection test strip and detection method thereof
  • Early pregnancy detection test strip and detection method thereof
  • Early pregnancy detection test strip and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 A kind of early pregnancy test strip

[0022] Such as figure 1 As shown, it is a structural schematic diagram of a test strip for early pregnancy detection of the present invention, and the test strip is sequentially lapped and pasted on the bottom plate 3 by the sample pad 1, the marking pad 2, the nitrocellulose membrane 4, and the absorbent paper 8 Composition, the mouse anti-β-HCG monoclonal antibody labeled with colloidal gold is coated on the described marker pad 2, and the nitrocellulose membrane 4 includes the detection area 5 coated with the mouse anti-α-HCG monoclonal antibody and the coating There is a control area 7 of anti-mouse IgG polyclonal antibody, a contrast area 6 coated with drug antigen is also arranged between the detection area 5 and the control area 7, and a colloidal gold label is also coated on the label pad 2 Antibodies to the drug.

[0023] In this example, the drug is THC (cannabis). The drug antigen coated in the contrast a...

Embodiment 2

[0035] Embodiment 2 A kind of early pregnancy test strip

[0036]The structure of the test strip of this embodiment is the same as that of Example 1, except that the drug is MET (ice). The drug antigen coated in the contrast area is MET antigen, and the coating concentration of the antigen is 0.25 mg / ml. The concentration of colloidal gold-labeled mouse anti-β-HCG monoclonal antibody coated on the marker pad, the concentration of mouse anti-α-HCG monoclonal antibody coated in the detection area, and the concentration of anti-mouse IgG polyclonal antibody coated in the control area were all equal. 1.0 mg / ml, and the coating amount was 0.15 μl / cm.

Embodiment 3

[0037] Embodiment 3 A kind of early pregnancy test strip

[0038] The structure of the test strip of this embodiment is the same as the structure of the test strip of Example 1, except that the drug is ketamine. The drug antigen coated in the contrast area is ketamine antigen, and the coating concentration of the antigen is 0.5 mg / ml. The concentration of colloidal gold-labeled mouse anti-β-HCG monoclonal antibody coated on the marker pad, the concentration of mouse anti-α-HCG monoclonal antibody coated in the detection area, and the concentration of anti-mouse IgG polyclonal antibody coated in the control area were all equal. It was 1.5mg / ml, and the coating amount was 0.2μl / cm.

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Abstract

The invention discloses an early pregnancy detection test strip and a detection method thereof. The test strip consists of a sample pad, a label pad, a nitrocellulose membrane and water absorption paper which are sequentially overlapped and adhered to a bottom plate, wherein a colloidal gold labeled anti-rat beta-HCG monoclonal antibody is coated on the label pad; the nitrocellulose membrane comprises a detection area coated with an anti-rat alpha-HCG monoclonal antibody and a control area coated with an anti-rat IgG polyclonal antibody; a contrast area coated with a drug antigen is formed between the detection area and the control area; and a colloidal gold labeled drug antibody is coated on the label pad. On the basis of the conventional early pregnancy detection test strip, the contrast area is formed between the detection area and the control area, so that the aim of predicting the pregnant time is fulfilled by comparing colors in the contrast area and the detection area; and moreover, the detection method is simple and is suitable for wide popularization.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to an early pregnancy detection test strip and a detection method thereof. Background technique [0002] Most women may experience dizziness, fatigue, drowsiness, increased salivation, loss of appetite, nausea and vomiting after 6 to 8 weeks of pregnancy. Vomiting often occurs in the morning or on an empty stomach. Some pregnant women especially like to eat acidic and cold food. The above-mentioned phenomena are called early pregnancy reactions, and they usually disappear on their own about 12 weeks after pregnancy. A few people's early pregnancy reactions are more serious and last longer. [0003] At present, most of the early pregnancy test papers on the market are detected by colloidal gold method, but all of them can only tell whether the subject is pregnant or not, and cannot know the approximate time of pregnancy. For some women who are pregnant by accident, there is no obvio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 王继华张亮唐时幸郭诗静
Owner GUANGZHOU WONDFO BIOTECH