Group of oligonucleotides aptamers capable of specifically recognizing aflatoxin B1

A kind of aflatoxin and oligonucleotide technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., to achieve the effect of simple operation, low preparation cost and stable single-stranded oligonucleotide
CN102952802AActive Publication Date: 2013-03-06JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2013-03-06

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Abstract

The invention discloses a group of oligonucleotides aptamers capable of specifically recognizing aflatoxin B1. A single-chain deoxyribonucleic acid (DNA) oligonucleotides aptamer capable of specifically recognizing the aflatoxin B1 is acquired by a systematic evolution of ligands by exponential enrichment (SELEX) technology; the aptamer can be transformed into a report aptamer by a fluorescein mark and the like; the report aptamer is used for detecting the aflatoxin B1; and the aptamer sequence can accurately and rapidly check the aflatoxin B1 in the food; and therefore, the oligonucleotides aptamer has the wide application prospect.
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Description

Technical field

[0001] The present invention relates to the field of biotechnology, in particular to the use of SELEX technology (exponentially enriched ligand system evolution technology) in molecular biology technology to prepare a set of aflatoxin B 1 The nucleic acid aptamer with high specificity and high affinity is used for the detection of aflatoxin B 1 The application in provides a scientific basis and theoretical basis. Background technique

[0002] Aflatoxins (AF) are mainly secondary metabolites of Aspergillus flavus and Aspergillus parasiticus, and their chemical structures are similar. They are all derivatives of dihydrofuranocoumarin. The structure of aflatoxin has been determined to be AFB 1 , AFB 2 , AFM 1 Among other 18 species, their basic structures all contain difuran ring and oxaphthalone (also known as coumarin). The former is a toxic structure, and the latter may be related to its carcinogenesis. Aflatoxin is resistant to high temperatures, and usually heat...

Claims

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