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Method for increasing growth speed of ganoderma lucidum mycelia and liquid fermentation biomass

A growth rate, liquid fermentation technology, applied in the field of microbial genetics and breeding, can solve the problems of low biomass and slow growth rate of breeding mycelium, and achieve the effect of high biomass, favorable for rapid growth and enhanced sensitivity

Inactive Publication Date: 2013-03-20
XUZHOU UNIV OF TECH
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for improving the growth rate of ganoderma lucidum mycelium and the biomass of liquid fermentation, so as to solve the problems of slow growth rate and low biomass of selected breeding mycelia in the prior art

Method used

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Embodiment 1

[0023] 1. Strains: Ganoderma lucidum ( Ganoderma lucidum ): purchased from China General Microorganism Culture Collection Management Center, No. 5.786.

[0024] 2. Culture medium: the peanut meal slant medium and the peanut meal liquid fermentation medium require grain materials including corn and peanut meal, purchased from supermarkets, corn and peanut meal crushed to a particle size of 100 μm.

[0025] The composition of peanut meal slant medium is corn 10~30g / L, peanut meal 2~5g / L, KH 2 PO 4 1~3g / L,MgSO 4 ·7H 2 O 0.5~1.5 g / L, VB 2 0.3~0.8g / L, agar 20g / L.

[0026] The peanut meal liquid fermentation medium consists of corn 10~30 g / L, peanut meal 2~5 g / L, peptone 1~3 g / L, glucose 10~30 g / L, yeast extract 1~3 g / L, K H 2 PO 4 1~3g / L,MgSO 4 ·7H 2 O 0.5~1.5 g / L, pH 6.5.

[0027] 3. Mutagenesis of Ganoderma lucidum protoplasts with nitrous acid

[0028] The ganoderma lucidum strain was incubated and activated at 28°C for 7 days, and the activated bacterial mass was...

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Abstract

The invention discloses a method for increasing the growth speed of ganoderma lucidum mycelia and the liquid fermentation biomass, and belongs to microbial genetics and breeding methods. The method comprises the steps as follows: (1) preparing a ganoderma lucidum protoplast; (2) carrying out induced mutation on the ganoderma lucidum protoplast by nitrous acid and regenerating the protoplast; (3) inoculating peanut meal and culturing by a slant culture medium comprising maize, the peanut meal, KH2PO4, MgSO4.7H20 and VB2; (4) inoculating the peanut meal and culturing by a liquid fermentation culture medium comprising the maize, the peanut meal, peptone, glucose, yeast cream, KH2PO4 and MgSO4.7H20; and (5) obtaining active products such as biomasses and polysaccharides, triterpenoids and the like. The method has the benefits as follows: (1) a ganoderma lucidum strain with high mycelial growth speed and high biomass is obtained through induced mutation for the first time; (2) the adopted slant culture medium for the peanut meal is more favorable for quick growth of the ganoderma lucidum mycelia in comparison with a slant culture medium for potato dextrose agar; and (3) the adopted liquid fermentation culture medium for the peanut meal is more favorable for acquisition of higher biomass.

Description

technical field [0001] The invention relates to a method for genetic breeding of microorganisms, in particular to a method for increasing the growth rate of ganoderma mycelium and the biomass of liquid fermentation. Background technique [0002] Contains more than ten active ingredients such as Ganoderma lucidum polysaccharides, triterpenoids, and Ganoderma lucidum polypeptides. Experiments have proved that Ganoderma lucidum has anti-inflammatory, analgesic, sedative, anti-aging, anti-tumor, anti-hypoxic, and immune-enhancing effects. Liquid submerged fermentation technology is an important way to obtain the main pharmacological active substances of Ganoderma lucidum, but on the one hand, the growth rate of Ganoderma lucidum mycelia is slow, which does not meet the large demand for Ganoderma lucidum strains; Active substance yield in filaments. Therefore, the key to solving the problem is to breed ganoderma strains with fast mycelium growth rate and high biomass. Protoplas...

Claims

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Application Information

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IPC IPC(8): A01G1/04C12N15/01C05G3/00
Inventor 苗敬芝董玉玮陈尚龙李勇
Owner XUZHOU UNIV OF TECH
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