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Preparation method of glossy ganoderma bioactive peptide

A technology of active peptides and Ganoderma lucidum, applied in the biological field, can solve the problems that Ganoderma lucidum peptides are rarely involved, and achieve the effects of good reproducibility, simple process and large sample volume.

Inactive Publication Date: 2013-03-27
TIBET JINKE GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction, purification and pharmacological effects of Ganoderma lucidum polysaccharides and Ganoderma triterpenoids are thoroughly researched at home and abroad, but the research on Ganoderma lucidum peptide (GLP) is rarely involved.

Method used

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  • Preparation method of glossy ganoderma bioactive peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Water extraction of Ganoderma lucidum mycelium powder

[0030] The mycelium powder of Ganoderma lucidum was extracted twice with distilled water at room temperature according to the solid-liquid ratio of 1:3 (W:V), 4 hours each time.

[0031] 2. Ultrafiltration separation

[0032] A hollow fiber membrane with a molecular weight cut-off of 10000 Da is used to conduct ultrafiltration separation on the water extract of Ganoderma lucidum mycelium powder, and obtain an ultrafiltrate containing low-molecular compounds such as amino acids, peptides and small molecular sugars.

[0033] 3. Activated carbon decolorization

[0034] 1% activated carbon was added to the ultrafiltrate for sufficient adsorption, filtered, and centrifuged at 5000 rpm for 15 minutes.

[0035] 4. Sephadex coordination chromatography separation

[0036] Take a certain amount of decolorized ultrafiltrate on Cu 2+ -SephadexG-25 column (φ1.6×70cm), eluted with borax buffer solution (pH value 11, 50mmo...

Embodiment 2

[0044] 1. Water extraction of Ganoderma lucidum mycelium powder

[0045] The mycelium powder of Ganoderma lucidum was extracted with distilled water at room temperature for 3 times at a solid-to-liquid ratio of 1:3 (W:V), 4 hours each time.

[0046] 2. Ultrafiltration separation

[0047] A hollow fiber membrane with a molecular weight cut-off of 10000 Da is used to conduct ultrafiltration separation on the water extract of Ganoderma lucidum mycelium powder, and obtain an ultrafiltrate containing low-molecular compounds such as amino acids, peptides and small molecular sugars.

[0048] 3. Activated carbon decolorization

[0049] Add 1% activated carbon to the ultrafiltrate to fully absorb, filter, and centrifuge at 5000rpm for 12min.

[0050] 4. Sephadex coordination chromatography separation

[0051] Take a certain amount of decolorized ultrafiltrate on Cu 2+ -SephadexG-25 column (φ1.6×70cm), eluted with borax buffer solution (pH value 11, 50mmol / L) at a flow rate of 0.2mL...

Embodiment 3

[0059] 1. Water extraction of Ganoderma lucidum mycelium powder

[0060] The ganoderma lucidum mycelium powder was extracted with distilled water at room temperature for 3 times at a solid-to-liquid ratio of 1:4 (W:V), 5 hours each time.

[0061] 2. Ultrafiltration separation

[0062] A hollow fiber membrane with a molecular weight cut-off of 10000 Da is used to conduct ultrafiltration separation on the water extract of Ganoderma lucidum mycelium powder, and obtain an ultrafiltrate containing low-molecular compounds such as amino acids, peptides and small molecular sugars.

[0063] 3. Activated carbon decolorization

[0064] 1.5% activated carbon was added to the ultrafiltrate for full adsorption, filtered, and centrifuged at 5000 rpm for 15 minutes.

[0065] 4. Sephadex coordination chromatography separation

[0066] Take a certain amount of decolorized ultrafiltrate on Cu 2+ -SephadexG-25 column (φ1.6×70cm), eluted with borax buffer solution (pH value 11, 50mmol / L) at a ...

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Abstract

The invention discloses a preparation method of glossy ganoderma bioactive peptide. The preparation method is characterized in that a glossy ganoderma mycelium powder is used as a raw material and is subjected to aqueous extraction, ultrafiltration separation, activated carbon decoloration, Cu<2+>-SephadexG-25 column coordination chromatograph, chelate resin copper removal, macroporous resin desalination and freeze drying to obtain the glossy ganoderma bioactive peptide. The glossy ganoderma bioactive peptide product provided by the invention contains peptides of more than 92% and has efficacies of liver protection, antioxidation, antifatigue, tumor inhibition and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of ganoderma lucidum active peptide, in particular to a method for preparing ganoderma lucidum active peptide by using modern biotechnology with ganoderma mycelium powder as raw material. Background technique [0002] Ganoderma lucidum belongs to Basidiomycetes, Polyporaceae, Polyporaceae Ganoderma plants. Ganoderma lucidum is also known as Ruicao, commonly known as "immortal grass", and the Japanese call it Wanniancao. Ganoderma lucidum was first recorded in "Shen Nong's Materia Medica" in the Eastern Han Dynasty and was listed as a herbal medicine. The so-called upper medicine means that it can be used as the monarch medicine in traditional Chinese medicine or compound prescriptions, that is, the main medicine, and it is believed that "the main medicine nourishes life and responds to heaven". Moreover, the toxicity is very low, "If you take it more, it will not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/16
Inventor 王秀英
Owner TIBET JINKE GRP
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