Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of mannitol in preparation of anti-breast cancer drug

An anti-breast cancer, mannitol technology, applied in drug combination, anti-tumor drugs, drug delivery, etc., can solve the problems of clinical application precedents without mannitol anti-tumor treatment

Inactive Publication Date: 2013-04-03
广西壮族自治区肿瘤防治研究所
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on whether mannitol can induce apoptosis of cancer cells at home and abroad, and there is no precedent of clinical application of mannitol for anti-tumor therapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of mannitol in preparation of anti-breast cancer drug
  • Application of mannitol in preparation of anti-breast cancer drug
  • Application of mannitol in preparation of anti-breast cancer drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Basic experimental research on mannitol's anti-tumor effect (lung cancer)

[0036] 1 Experimental materials

[0037] 1.1 Materials

[0038] 1.1.1 Cell line: SPC-A-1 human lung cancer cell line was obtained from Shanghai Cancer Institute.

[0039] 1.1.2 Reagents: RPMI 1640 and trypsin were purchased from Gibcol BRL; newborn bovine serum was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.; sodium bicarbonate (analytical grade) was produced by Hebei Cizhou Pharmaceutical Factory; sodium penicillin and streptomycin sulfate Su is produced by Harbin Pharmaceutical Group Pharmaceutical General Factory; thiazolyl blue (MTT) and dimethyl sulfoxide (DMSO) are products of Sigma; ATP kits (ATP extract, luciferin-luciferase, diluent, etc.) are German DCS The company's products; sodium citrate was produced by Guangzhou Chemical Reagent Factory; propidium iodide (PI) and RNaseA were products of Sigma; mannitol injection was purchased from Guangxi Nannin...

Embodiment 2

[0058] Example 2 Study on the Apoptosis of Lung Adenocarcinoma Cells Induced by a Specific Concentration of Mannitol and Its Mechanism (Lung Adenocarcinoma)

[0059] 1 Materials and methods

[0060] 1.1 Main reagents and instruments: DMEM (high glucose) culture medium (subpackaged by American hyclone company Thermo Fisher Biochemical Beijing Co., Ltd.), trypsin, newborn calf serum (FBS purchased from Hangzhou Sijiqing Biological Co., Ltd.) , 20% mannitol (purchased from Anshuanghe Pharmaceutical Co., Ltd.), Giemsa staining kit (provided by the Testing Center of the First Affiliated Hospital of Guangxi Medical University), apoptosis hoechst 33342 kit (purchased from Shanghai Beyontine Biotechnology Co., Ltd.) apoptosis Dapi staining kit, flow cytometry kit (both purchased from Nanjing Kaiji Biotechnology Development Co., Ltd.), Bcl-2 detection kit (purchased from Fuzhou Maixin Biotechnology Development Co., Ltd.), Pentylene glycol and transmission electron microscopy were prov...

Embodiment 3

[0101] Embodiment 3 breast cancer

[0102] Referring to Example 1, the MTT method was used to determine the cell inhibitory rate of a specific concentration of mannitol on breast cancer cells. The results showed that within 48 hours, mannitol could inhibit the proliferation of breast cancer cells in the concentration range of 1-150 μg / μl, and its inhibitory effect was enhanced with the increase of the concentration. Within 5 days, the inhibition rate decreased with the increase of concentration and time. Also increasing.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of mannitol in preparation of an anti-breast cancer drug, and in particular relates to a use of a mannitol injection with a specific concentration of 10%-19% for treating breast cancer. Traditionally, mannitol is only used as a diuretic, a high-permeability hypotensor and the like, while the inventor discovers through scientific experiments that mannitol has anti-tumor activity, and through several cell apoptosis detection technologies and by detecting apoptosis-related genes bcl-2, and bax, proves that the specific-concentration mannitol can induce various human cancer cell strains to generate obvious cancer cell apoptosis, grasps the effect relation with concentration and time, and first illustrates the possible mechanism; furthermore, the inventor has used the research result to tumor patient treatment and has achieved obvious curative effect, which provides theoretical basis and practical cases for the application of mannitol in preparation of the anti-tumor drug and the clinical generalization thereof.

Description

technical field [0001] The invention relates to a new application of mannitol, in particular to the application of mannitol in the preparation of anti-breast cancer drugs. Background technique [0002] At present, in anti-tumor research, inducing the apoptosis of cancer cells is an important way. Promoting tumor cell apoptosis can achieve the purpose of tumor shrinkage and cancer regression. For example, traditional anti-tumor drugs cisplatin and melphalan can all Stimulates the mitochondrial apoptotic pathway in cells, triggering apoptosis. This method has obvious advantages over the treatment method of killing tumor cells, because if anti-tumor drugs can induce the apoptosis of tumor cells in large quantities and activate their own programmed death process, it will avoid the release of tumor cells due to a large number of necrotic cells. Various side effects caused by intracellular substances can reduce the damage of chemotherapy to normal cells and prolong the survival o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/047A61K9/08A61P35/00
Inventor 黄鼎铭刘德森黎乐群黄亚铭张力图曹骥黄其春吴军岳惠芬李黄羿潘琪黎斌杨立黄亚芬茅乃权左传田谢彤潘泓黄耀元欧超梁新强崔英
Owner 广西壮族自治区肿瘤防治研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com