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Entomogenous fungus Nomuraearileyi, as well as culture method and application thereof

A technology of Nomura reinhardtii and insect-borne fungi is applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve problems such as different insecticidal effects, and achieves low cost, reduced production cost, and preparation method. simple effect

Inactive Publication Date: 2013-05-08
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, Nomura strains from different sources not only have obvious differences in colony growth, spore production and spore germination rate, but also have different insecticidal effects. However, spore production, spore germination rate, insecticidal Effect etc. are important factors affecting the application of Nomura strains in agricultural biocontrol

Method used

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  • Entomogenous fungus Nomuraearileyi, as well as culture method and application thereof
  • Entomogenous fungus Nomuraearileyi, as well as culture method and application thereof
  • Entomogenous fungus Nomuraearileyi, as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Separation of strains

[0025] Collection of strains: from 2009 to 2010, rehmannia glutinosa and peanuts from Wang Junxia Experimental Field in Tan District, Wenxian County, Experimental Field of Wenxian Agricultural Science Institute, and Modern Agricultural Science and Technology Experimental Base of Henan Academy of Agricultural Sciences (abbreviation: Henan Academy of Agricultural Sciences Base) , Soybean field to collect diseased zombies.

[0026] Isolation of strains: the conventional method of isolating entomogenic fungal tissues was adopted. Rinse the surface of the worm body with clean water, soak it in 75% alcohol for 2 seconds, rinse it twice with sterile water, then cut the worm body into small pieces with a sterilized scalpel and tweezers, soak it in 75% alcohol for 1.5 min, no Soak in bacterial water twice, each time for 10s, then connect the worm body block to the surface of the PDA plate with sterile tweezers, and keep it moist at 28°C. The...

Embodiment 2

[0031] Embodiment 2 strain identification

[0032] For the isolated bacterial strain Nr1001 in Example 1, through the observation of biological characteristics, further morphological identification is carried out, the method is as follows:

[0033] (1) Morphology and culture characteristics: culture on PDA medium at 28°C for 10 days, observe the colony characteristics and color, etc., and observe the characteristics of the bacteria with an optical microscope.

[0034] (2) ITS sequence analysis: Scrape the hyphae cultured on the PDA plate, extract the genomic DNA of the strain according to the conventional method, synthesize general PCR primers for amplifying the fungal ITS sequence, and amplify the target fragment using the routine method of molecular biology Methods for cloning and sequencing. The sequencing results were analyzed by BLAST (http: / / www.ncbi.nlm.nih.gov / ), and compared with the sequences in the GenBank database to determine the taxonomic status of the strain....

Embodiment 3

[0041] The biological characteristic of embodiment 3 bacterial strains

[0042] The bacterial strain identified in example 2 is carried out the research of biological characteristic, method is as follows:

[0043] (1) Effects of nutritional conditions on the growth and sporulation of Nomura reyesi strain Nr1001. Different C sources, N sources, C / N, trace elements, and vitamins were inserted into the solid plate culture medium of Nr1001 cultured on PDA medium. Mycelia block and 50ml liquid culture medium of different C sources, N sources, C / N, trace elements, and vitamins were added to 100 μL of spore suspension (concentration at 1×10 7 cells / ml), after ten days of cultivation, the colony diameter, spore production and dry mycelium weight under liquid culture conditions were measured, and the recorded experimental results were analyzed with DPS and SPSS software.

[0044] (2) Effect of culture conditions on the growth and sporulation of Nomuraella reyesii Nr1001 strain Set dif...

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Abstract

The invention relates to entomogenous fungus Nomuraearileyi, as well as a culture method and an application thereof. The collection number of the Nomuraearileyi Nr1001 is CGMCCNO. 6387. A microbial agent is formed by mixing a Nomuraearileyi Nr1001 fermentation solution, a surfactant and an anti-corrosion agent. A screened Nr1001 strain has the advantages of broad insecticidal spectrum and good insecticidal effect and stronger dissemination against lepidoptera noctuidae insects, such as larvae of cotton bollworm, asparagus caterpillar, prodenia litura, three-spotted plusia, soybean looper, oriental tobacco budworm and the like, and can prevent the prevalence of field pests and plant diseases, effectively reduce the population density of the pests and realize a better pest control effect.

Description

technical field [0001] The invention relates to the field of biological control, in particular to an insecticidal fungus-Nomura reinerii and its cultivation method and application. Background technique [0002] Compared with other insecticidal microorganisms, fungi have their special advantages: contact killing, prevalence, environmental safety and resistance to pesticides; simple cultivation and production, easy industrialization; underground pests that cannot be controlled by Bt pesticides, There are special effects on the control of borer pests and piercing-sucking mouthparts pests, etc. Therefore, the research and development of fungal insecticides has attracted extensive attention and attention of researchers. [0003] Nomura Reidi[ Nomuraea rileyi (Farlow)Samson] is a widely distributed entomopathogenic fungus, which can parasitize more than 40 kinds of insects, and is particularly pathogenic to the larvae of Lepidoptera Noctuidae pests. It can cause cotton bollworm a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P7/04C12R1/645
Inventor 任应党崔筱张书杰刘新涛王刚刘玉霞赵辉刘红彦
Owner HENAN ACAD OF AGRI SCI
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