Preparation method and use of graft material in double membrane structure

Abstract

The invention belongs to the field of tissue engineering and biomaterials and discloses a preparation method and use of a graft material in a double membrane structure. The graft material comprises stem cell membrane patch segments and platelet-rich fibrin membrane particles, wherein the stem cells are firstly expanded-cultured, and then induction-cultured by using a membrane induction fluid to obtain a stem cell membrane patch, and the stem cell membrane patch is sheared to prepare the cell membrane patch segment; a platelet-rich fibrin gel is extruded, then liquid content of the extruded platelet-rich fibrin gel is removed to obtain the platelet-rich fibrin membrane, and the platelet-rich fibrin membrane is sheared to form particles; and the cell membrane patch segments and the platelet-rich fibrin membrane particles are mixed according to an optimal proportioning relation of in-vitro screening to prepare the needed graft material. The graft material can be widely applied to tissue repair and regeneration of small-area lesion in oral activity and other parts of the body, and the tissue repair effect is improved.

Description

technical field [0001] The invention belongs to the field of tissue engineering and biomaterials, and relates to a double-membrane structure transplant material, in particular to a preparation method and application of a double-membrane structure transplant material compounded with platelet-rich fibrin membrane by stem cell membrane fragments. Background technique [0002] At present, tissue defect repair using stem cell technology usually adopts the method of compounding stem cell suspension and exogenous scaffold material. There are three main forms: simple cell suspension local transplantation; cell suspension inoculation on exogenous scaffold material; cell suspension Three-dimensional culture was carried out in vitro after seeding on the scaffold material. However, there are many shortcomings in the above-mentioned transplantation method: firstly, during the process of cells being digested with trypsin to obtain the suspension, the structure of intercellular signaling m...

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Problems solved by technology

However, there are many shortcomings in the above-mentioned transplantation method: firstly, during the process of cells being digested with trypsin to obtain the suspension, the structure of intercellular signaling molecules and extracellular matrix is ​​destroyed, and the activity of the cells themselves is also affected; secondly, the cells The amount of transplantation is difficult to control. If the amount of cells is too small, it is difficult for cells to quickly colonize the transplanted area. If the amount of cells is too large, it will cause local cell accumulation, which is not conducive to cell stretching and nutrient supply. Finally, the transplantation and degradation of scaffold materials may cause host Toxic reactions and immune reactions, while undegraded scaffold materials can cause fiber wrapping, affecting blood supply and tissue structure reconstruction

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