Application of SMOC2 gene in preparation of medicine for detecting or treating endometrial cancer and ovarian cancer

A technology for endometrial cancer and drug preparation, applied in gene therapy, drug combination, biochemical equipment and methods, etc., can solve unknown problems and achieve the effects of reliable results, good repeatability, great application value and social benefits

Inactive Publication Date: 2013-05-15
FENGXIAN CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanism of action of SMOC2 gene in the microenvironment of gynecological tumors, as well as its specific receptors or intera...

Method used

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  • Application of SMOC2 gene in preparation of medicine for detecting or treating endometrial cancer and ovarian cancer
  • Application of SMOC2 gene in preparation of medicine for detecting or treating endometrial cancer and ovarian cancer
  • Application of SMOC2 gene in preparation of medicine for detecting or treating endometrial cancer and ovarian cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1, the expression of SMOC2 in endometrial cancer patient tissues

[0057] main reagent

[0058] SMOC2 polyclonal antibody (ab78069) and secondary antibody polyclonal anti-rabbit IgG (ab6721) were purchased from abcam company.

[0059] 1.1 Construction of endometrial cancer tissue chip array

[0060] 1.2 Immunohistochemical staining

[0061] 1.4 Bake the paraffin sections in an oven at 80°C for 20 minutes before staining, and then press xylene for 10 minutes, 1 / 2 xylene for 10 minutes, 100% alcohol for 10 minutes, 95% alcohol for 10 minutes, 85% alcohol for 10 minutes, and 75% alcohol for 10 minutes. Dewaxing. The dewaxed sections were stained according to the following procedure: rinse with tap water for 5-10 minutes, antigen retrieval for 10 minutes, wait for natural cooling, wash with 1×PBS in 0.3% H2O2 at 37°C for 3 minutes, then block with 10% BSA at room temperature for 1 hour and incubate with primary antibody Overnight at 4°C, wash 3 times with 1×PBS ...

Embodiment 2

[0069] Using lentiviral interference vector to interfere with SMOC2 gene in endometrial cancer cell line and conduct experimental research on cell function

[0070] The SMOC2 gene uses the complete coding region (open reading frame) of human SMOC2 cDNA as the target gene, and the sequence of the complete coding region is the same as the ORF sequence (NCBI Reference Sequence: NM_022138.2) 1374bp in the content of the invention. For the encoded protein, please refer to Sequence Table 1 (SEQ ID NO.1).

[0071] 2.1 Main reagents

[0072]Endometrial cancer cell lines HEC-1A, HEC-1B, RL-952, AN3CA, and Ishikawa were purchased from the Cell Bank of the Chinese Academy of Sciences, the cell culture medium and fetal bovine serum were purchased from Gibco, and the SMOC2 gene RNA interference lentiviral expression vector was purchased from Shanghai Gemma Pharmaceutical Technology Co., Ltd. The RNA extraction reagent RNAiso Plus was purchased from Bao Biological Engineering Co., Ltd., t...

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Abstract

The invention provides application of SMOC2 gene (SPARC related modular calcium binding2, with the Chinese name of secreting modular calcium binding protein 2; smooth muscle related protein 2) in preparation of medicine for detecting or treating endometrial cancer and ovarian cancer. A tissue chip comprising 157 endometrial cancer tissue specimens, 30 normal proliferative phase endometrium tissue specimens and 30 normal secretory phase endometrium tissue specimens is established by utilizing a tissue microarray technology, the expression conditions of SMOC2 in the specimens are detected by utilizing the immunohistochemical technique, the positive expression rate of the SMOC2 in the normal tissue of the endometrium is far lower than the positive expression rate in the endometrial cancer, and the expression intensity is positively correlated to muscular invasion depth of the endometrial cancer and tumor grading of the endometrial cancer. Therefore, the SMOC2 gene can be used for preparing the medicine for detecting or treating endometrial cancer. The medicine is reliable in experimental result and high in repeatability and has good clinical application prospect and good application values and social benefits.

Description

technical field [0001] The invention relates to medicines, in particular to biological medicines, especially to the application of SMOC2 gene in the preparation of medicines for detecting or treating endometrial cancer. Background technique [0002] Endometrial cancer is one of the three major gynecological malignant tumors. In recent years, the incidence rate has been on the rise, and the age of onset is getting younger (KOUJI BANNO et al. [0003] Biomarkers in endometrial cancer: Possible clinical applications). Risk factors for endometrial cancer include obesity, infertility and infertility, late menopause, diabetes, and high blood pressure. The occurrence of endometrial cancer is directly related to the continuous effect of estrogen. The pathogenesis is unknown. But it is clear that the occurrence and metastasis of endometrial cancer are closely related to the environment in which tumor cells live. Studies have shown that collagen, laminin and their receptor integri...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68G01N33/574A61K48/00A61K39/00A61K39/395A61P35/00
Inventor 张蓉张学利杨广东朱琳艳陆欢
Owner FENGXIAN CENT HOSPITAL
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