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Response gene BrPHYB for controlling shadowing property of cabbage and application thereof

A gene and reaction technology, applied in the field of molecular biology, can solve the problems of plant heightening and branch reduction

Active Publication Date: 2013-05-22
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under the condition of dense planting of Chinese cabbage, the plants will compete with the surrounding plants and the plants will show shade-avoidance responses, such as: plant height, reduced branching, internode elongation, and flowering acceleration. , repairing the shade-avoidance response of cabbage has become an important target for genetic modification

Method used

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  • Response gene BrPHYB for controlling shadowing property of cabbage and application thereof
  • Response gene BrPHYB for controlling shadowing property of cabbage and application thereof
  • Response gene BrPHYB for controlling shadowing property of cabbage and application thereof

Examples

Experimental program
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Effect test

Embodiment

[0021] Isolation and Transformation of Genes

[0022] Select the aerial part of the 10-day-old seedlings of Chinese cabbage (Fushan Baotou) grown in the greenhouse as the material, put it in a mortar pre-cooled with liquid nitrogen, add liquid nitrogen to quickly grind it into powder, put 100mg of the powder into a 1.5ml centrifuge tube, add 1ml of Trizol reagent (sold by Invitrogen) was mixed upside down, and allowed to stand at room temperature for 10 minutes. Centrifuge at 12,000 rpm for 10 minutes at 4°C, and transfer the supernatant to a new 1.5ml centrifuge tube. Add 200 μl of chloroform, shake for 15 seconds, and let stand at room temperature for 2 to 3 minutes. Centrifuge at 12000 rpm for 10 minutes at 4°C. Take the colorless upper aqueous phase into a new centrifuge tube, add 600 μl -20 ℃ refrigerator pre-cooled isopropanol, invert and mix well, and let stand at room temperature for 10 minutes. Centrifuge at 12,000 rpm for 10 minutes at 4°C, and aspirate the supern...

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Abstract

The invention relates to a response gene BrPHYB for controlling shadowing property of cabbage and the application thereof. The gene BrPHYB has a nucleotide sequence shown in SEQ ID NO.1 and 3519 basic groups in total length. The invention further relates to the application of the gene in commercial crop production improvement. The gene BrPHYB disclosed by the invention is a plant shadowing property response control gene which can restrain shadowing property response, and a crop which restrains shadowing property response can be obtained by over-expressing the gene.

Description

technical field [0001] The invention relates to a gene controlling the shade avoidance response of Chinese cabbage, in particular to a BrPHYB gene regulating the shade avoidance response of Chinese cabbage and its application, belonging to the technical field of molecular biology. Background technique [0002] Cabbage (Brassica rapa L.ssp.pekinensis) originated in China and is one of the important vegetable crops in my country and even in the world. The leaf is the main edible organ of Chinese cabbage, and improving the leaf yield of Chinese cabbage is an important economic trait to be considered in the current work of Chinese cabbage breeding. Close planting is an important measure to increase the photosynthetic area. However, under the condition of dense planting of Chinese cabbage, the plants will compete with the surrounding plants and the plants will show shade-avoidance responses, such as: plant height, reduced branching, internode elongation, and flowering accelerati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/63C07K14/415A01H5/00
Inventor 宋梅芳张庶杨建平高建伟
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI