Trichodermalongibrachiatum GF-10 and application thereof

A technology of Trichoderma longibrachiae, which is applied in the direction of application, fungicides, fungi, etc., can solve the problems of no relevant reports on biological control, and achieve the effects of easy cultivation and preservation, reasonable technical route, and strong tolerance

Inactive Publication Date: 2013-05-29
BIJIE COMPANY OF GUIZHOU TOBACCO +1
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

And there is no relevant report on the biological contro

Method used

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[0030] detailed description

[0031] Isolation steps of Trichoderma longiflorum strain GF-10 which has inhibitory effect on tobacco black shank and Alternaria alternata:

[0032] (1) Test strain: Tobacco black shank-Phytophthora tabaci ( Phytophthora nicotianae Gzufp-25), Alternaria alternata (Alternaria alternata) ( Aiternaria alternate Gzufc-18) was isolated from Bijie City and kept in the Institute of Fungal Resources, College of Life Sciences, Guizhou University.

[0033] (2) Isolation and purification of antagonistic bacteria: accurately weighed 10.0g tobacco plant root soil samples collected from Qianxi County, Bijie City, Guizhou Province. With the help of aseptic technique, the soil samples were added to 90mL sterile water with glass In the bead flask, shake for 10 minutes to evenly disperse the soil sample into a diluent, and then pipette 1 mL of the soil diluent into 9 mL of sterile water, mix well to form a soil suspension. Inoculate by mixed bacteria method, pipette...

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Abstract

The invention discloses trichodermalongibrachiatum GF-10 and an application thereof. The morphological characteristics are as follows: the trichodermalongibrachiatum GF-10 grows rapidly on a PDA (Potato Dextrose Agar) and the back face grows yellow green from colorless; conidiums can be generated after the trichodermalongibrachiatum GF-10 is cultured at 28 DEG C for 72 hours; stem secondary branches of the conidiums are fewer and stretch out in a right angle generally; bottle stems directly stretch out from the secondary branches; solitary and dichotomy exist and whorls are very few; the bottle stems are in a calabash or flask type; the length is 5.3-11.6 microns and the width is 2.0-3.2 microns; the final bottle stem is conical; spores are grey green or green, and are oval or ellipse-shaped; and the length is 3.4-6.6 microns and the width is 2.3-3.5 microns. The trichodermalongibrachiatum GF-10 has strong tolerance on a bad environment, resists mutation, is easily cultured and stored, and has good effects on inhibiting phytophthora nicotiana and alternaria alternate.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Trichoderma longibrachiata strain, and also relates to the use of the Trichoderma longibranchium strain in inhibiting tobacco black shank and tobacco red spot fungus. [0002] Background technique [0003] Tobacco black shank is the most important rhizome disease in tobacco leaf production, while tobacco red spot is an important leaf disease in tobacco leaf production. The control of both is particularly important for tobacco leaf production. [0004] At present, there are many control methods for tobacco black shank and tobacco red spot, including cultivating disease-resistant varieties, chemical control, crop rotation and biological control. Planting disease-resistant varieties is the most economical and effective way to prevent and control the two diseases. However, when using disease-resistant varieties to prevent and control the two diseases, not only the resistance level and...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 葛永怡李祝张传平庄培林黄化刚黄青明申燕孙希文刘阳
Owner BIJIE COMPANY OF GUIZHOU TOBACCO
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